Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10035 and BWI76_RS10040 are separated by 36 nucleotides BWI76_RS10040 and BWI76_RS10045 are separated by 60 nucleotides BWI76_RS10045 and BWI76_RS10050 are separated by 190 nucleotides BWI76_RS10050 and BWI76_RS10055 are separated by 15 nucleotides
BWI76_RS10035: BWI76_RS10035 - hypothetical protein, at 2,126,976 to 2,127,908
_RS10035
BWI76_RS10040: BWI76_RS10040 - heat-shock protein HspQ, at 2,127,945 to 2,128,262
_RS10040
BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525
_RS10045
BWI76_RS10050: BWI76_RS10050 - outer membrane protein, at 2,129,716 to 2,130,270
_RS10050
BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074
_RS10055
Position (kb)
2128
2129
2130 Strain fitness (log2 ratio)
-1
0
1 at 2127.326 kb on + strand, within BWI76_RS10035 at 2127.835 kb on + strand at 2128.217 kb on - strand, within BWI76_RS10040 at 2128.495 kb on - strand, within BWI76_RS10045 at 2128.714 kb on - strand, within BWI76_RS10045 at 2128.762 kb on - strand, within BWI76_RS10045 at 2128.900 kb on - strand, within BWI76_RS10045 at 2129.803 kb on + strand, within BWI76_RS10050 at 2129.811 kb on - strand, within BWI76_RS10050 at 2129.980 kb on + strand, within BWI76_RS10050 at 2130.069 kb on - strand, within BWI76_RS10050 at 2130.140 kb on + strand, within BWI76_RS10050 at 2130.164 kb on + strand, within BWI76_RS10050 at 2130.246 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,127,326 + BWI76_RS10035 0.38 -0.6 2,127,835 + +0.3 2,128,217 - BWI76_RS10040 0.86 -0.0 2,128,495 - BWI76_RS10045 0.14 -1.2 2,128,714 - BWI76_RS10045 0.33 -0.8 2,128,762 - BWI76_RS10045 0.36 -0.7 2,128,900 - BWI76_RS10045 0.48 -1.2 2,129,803 + BWI76_RS10050 0.16 +0.3 2,129,811 - BWI76_RS10050 0.17 -0.4 2,129,980 + BWI76_RS10050 0.48 -1.4 2,130,069 - BWI76_RS10050 0.64 +0.2 2,130,140 + BWI76_RS10050 0.76 +0.3 2,130,164 + BWI76_RS10050 0.81 +1.1 2,130,246 + +0.4
Or see this region's nucleotide sequence