Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05685 and BWI76_RS05690 are separated by 143 nucleotides BWI76_RS05690 and BWI76_RS05695 are separated by 112 nucleotides
BWI76_RS05685: BWI76_RS05685 - dihydrodipicolinate synthase family protein, at 1,195,315 to 1,196,244
_RS05685
BWI76_RS05690: BWI76_RS05690 - fumarylacetoacetate (FAA) hydrolase, at 1,196,388 to 1,197,236
_RS05690
BWI76_RS05695: BWI76_RS05695 - anaerobic C4-dicarboxylate transporter, at 1,197,349 to 1,198,638
_RS05695
Position (kb)
1196
1197
1198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1195.441 kb on + strand, within BWI76_RS05685 at 1195.681 kb on + strand, within BWI76_RS05685 at 1195.691 kb on + strand, within BWI76_RS05685 at 1195.740 kb on + strand, within BWI76_RS05685 at 1195.887 kb on - strand, within BWI76_RS05685 at 1195.920 kb on + strand, within BWI76_RS05685 at 1195.926 kb on - strand, within BWI76_RS05685 at 1195.939 kb on - strand, within BWI76_RS05685 at 1195.945 kb on - strand, within BWI76_RS05685 at 1196.021 kb on + strand, within BWI76_RS05685 at 1196.030 kb on - strand, within BWI76_RS05685 at 1196.133 kb on + strand, within BWI76_RS05685 at 1196.146 kb on - strand, within BWI76_RS05685 at 1196.219 kb on + strand at 1196.232 kb on - strand at 1196.239 kb on + strand at 1196.243 kb on + strand at 1196.293 kb on - strand at 1196.363 kb on + strand at 1196.364 kb on - strand at 1196.368 kb on + strand at 1196.371 kb on - strand at 1196.374 kb on + strand at 1196.394 kb on + strand at 1196.466 kb on - strand at 1196.509 kb on + strand, within BWI76_RS05690 at 1196.509 kb on + strand, within BWI76_RS05690 at 1196.510 kb on + strand, within BWI76_RS05690 at 1196.517 kb on - strand, within BWI76_RS05690 at 1196.517 kb on - strand, within BWI76_RS05690 at 1196.517 kb on - strand, within BWI76_RS05690 at 1196.517 kb on - strand, within BWI76_RS05690 at 1196.519 kb on + strand, within BWI76_RS05690 at 1196.527 kb on + strand, within BWI76_RS05690 at 1196.527 kb on - strand, within BWI76_RS05690 at 1196.527 kb on - strand, within BWI76_RS05690 at 1196.531 kb on + strand, within BWI76_RS05690 at 1196.662 kb on - strand, within BWI76_RS05690 at 1196.723 kb on + strand, within BWI76_RS05690 at 1196.760 kb on + strand, within BWI76_RS05690 at 1196.800 kb on - strand, within BWI76_RS05690 at 1196.808 kb on - strand, within BWI76_RS05690 at 1196.826 kb on + strand, within BWI76_RS05690 at 1197.016 kb on - strand, within BWI76_RS05690 at 1197.077 kb on + strand, within BWI76_RS05690 at 1197.205 kb on - strand at 1197.275 kb on - strand at 1197.275 kb on - strand at 1197.321 kb on - strand at 1197.363 kb on + strand at 1197.381 kb on - strand at 1197.402 kb on + strand at 1197.415 kb on + strand at 1197.445 kb on + strand at 1197.559 kb on - strand, within BWI76_RS05695 at 1197.678 kb on - strand, within BWI76_RS05695 at 1197.716 kb on + strand, within BWI76_RS05695 at 1197.845 kb on + strand, within BWI76_RS05695 at 1197.853 kb on - strand, within BWI76_RS05695 at 1197.853 kb on - strand, within BWI76_RS05695 at 1197.853 kb on - strand at 1197.878 kb on + strand, within BWI76_RS05695 at 1197.974 kb on - strand, within BWI76_RS05695 at 1198.010 kb on + strand, within BWI76_RS05695 at 1198.053 kb on - strand, within BWI76_RS05695 at 1198.053 kb on - strand, within BWI76_RS05695
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 1,195,441 + BWI76_RS05685 0.14 -0.2 1,195,681 + BWI76_RS05685 0.39 -0.3 1,195,691 + BWI76_RS05685 0.40 -2.3 1,195,740 + BWI76_RS05685 0.46 -0.5 1,195,887 - BWI76_RS05685 0.62 -1.4 1,195,920 + BWI76_RS05685 0.65 +0.5 1,195,926 - BWI76_RS05685 0.66 -1.6 1,195,939 - BWI76_RS05685 0.67 +0.5 1,195,945 - BWI76_RS05685 0.68 -0.1 1,196,021 + BWI76_RS05685 0.76 -1.9 1,196,030 - BWI76_RS05685 0.77 -3.0 1,196,133 + BWI76_RS05685 0.88 -0.4 1,196,146 - BWI76_RS05685 0.89 +0.6 1,196,219 + +2.4 1,196,232 - -0.7 1,196,239 + +1.0 1,196,243 + -1.2 1,196,293 - +2.5 1,196,363 + +0.3 1,196,364 - +0.2 1,196,368 + +0.8 1,196,371 - -1.6 1,196,374 + +2.0 1,196,394 + -1.6 1,196,466 - +0.1 1,196,509 + BWI76_RS05690 0.14 -1.4 1,196,509 + BWI76_RS05690 0.14 -1.6 1,196,510 + BWI76_RS05690 0.14 -2.0 1,196,517 - BWI76_RS05690 0.15 +0.0 1,196,517 - BWI76_RS05690 0.15 -0.2 1,196,517 - BWI76_RS05690 0.15 -2.3 1,196,517 - BWI76_RS05690 0.15 -0.8 1,196,519 + BWI76_RS05690 0.15 -0.3 1,196,527 + BWI76_RS05690 0.16 -0.1 1,196,527 - BWI76_RS05690 0.16 -1.1 1,196,527 - BWI76_RS05690 0.16 -0.5 1,196,531 + BWI76_RS05690 0.17 -1.3 1,196,662 - BWI76_RS05690 0.32 -1.0 1,196,723 + BWI76_RS05690 0.39 -0.7 1,196,760 + BWI76_RS05690 0.44 -1.0 1,196,800 - BWI76_RS05690 0.49 -0.6 1,196,808 - BWI76_RS05690 0.49 -3.1 1,196,826 + BWI76_RS05690 0.52 -0.9 1,197,016 - BWI76_RS05690 0.74 -0.6 1,197,077 + BWI76_RS05690 0.81 +0.3 1,197,205 - +0.6 1,197,275 - -1.4 1,197,275 - -0.2 1,197,321 - -0.7 1,197,363 + -1.2 1,197,381 - -0.0 1,197,402 + -0.6 1,197,415 + -0.6 1,197,445 + +0.4 1,197,559 - BWI76_RS05695 0.16 -1.7 1,197,678 - BWI76_RS05695 0.26 +0.1 1,197,716 + BWI76_RS05695 0.28 -0.8 1,197,845 + BWI76_RS05695 0.38 -0.7 1,197,853 - BWI76_RS05695 0.39 -1.7 1,197,853 - BWI76_RS05695 0.39 -0.7 1,197,853 - +2.8 1,197,878 + BWI76_RS05695 0.41 +0.1 1,197,974 - BWI76_RS05695 0.48 +0.1 1,198,010 + BWI76_RS05695 0.51 -0.8 1,198,053 - BWI76_RS05695 0.55 +0.5 1,198,053 - BWI76_RS05695 0.55 +0.4
Or see this region's nucleotide sequence