Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04440 and BWI76_RS04445 overlap by 4 nucleotides BWI76_RS04445 and BWI76_RS04450 are separated by 10 nucleotides BWI76_RS04450 and BWI76_RS04455 are separated by 19 nucleotides
BWI76_RS04440: BWI76_RS04440 - citrate lyase ACP, at 917,395 to 917,688
_RS04440
BWI76_RS04445: BWI76_RS04445 - citrate (pro-3S)-lyase subunit beta, at 917,685 to 918,554
_RS04445
BWI76_RS04450: BWI76_RS04450 - citrate lyase subunit alpha, at 918,565 to 920,091
_RS04450
BWI76_RS04455: BWI76_RS04455 - triphosphoribosyl-dephospho-CoA synthase CitG, at 920,111 to 921,019
_RS04455
Position (kb) 918
919
920
921 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 917.615 kb on - strand, within BWI76_RS04440 at 917.653 kb on + strand, within BWI76_RS04440 at 917.759 kb on - strand at 917.935 kb on + strand, within BWI76_RS04445 at 918.031 kb on + strand, within BWI76_RS04445 at 918.031 kb on + strand, within BWI76_RS04445 at 918.046 kb on + strand, within BWI76_RS04445 at 918.379 kb on - strand, within BWI76_RS04445 at 918.454 kb on + strand, within BWI76_RS04445 at 918.527 kb on - strand at 918.741 kb on + strand, within BWI76_RS04450 at 918.749 kb on - strand, within BWI76_RS04450 at 918.914 kb on + strand, within BWI76_RS04450 at 918.914 kb on + strand, within BWI76_RS04450 at 918.938 kb on + strand, within BWI76_RS04450 at 919.454 kb on + strand, within BWI76_RS04450 at 919.509 kb on + strand, within BWI76_RS04450 at 919.529 kb on - strand, within BWI76_RS04450 at 919.639 kb on + strand, within BWI76_RS04450 at 920.000 kb on - strand at 920.081 kb on - strand at 920.159 kb on - strand at 920.251 kb on + strand, within BWI76_RS04455 at 920.281 kb on - strand, within BWI76_RS04455 at 920.283 kb on + strand, within BWI76_RS04455 at 920.350 kb on + strand, within BWI76_RS04455 at 920.372 kb on - strand, within BWI76_RS04455 at 920.479 kb on - strand, within BWI76_RS04455 at 920.497 kb on - strand, within BWI76_RS04455 at 920.689 kb on + strand, within BWI76_RS04455 at 920.728 kb on + strand, within BWI76_RS04455 at 920.873 kb on + strand, within BWI76_RS04455 at 920.952 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 917,615 - BWI76_RS04440 0.75 +0.2 917,653 + BWI76_RS04440 0.88 +1.0 917,759 - -0.3 917,935 + BWI76_RS04445 0.29 -0.3 918,031 + BWI76_RS04445 0.40 +0.2 918,031 + BWI76_RS04445 0.40 -0.1 918,046 + BWI76_RS04445 0.41 -2.4 918,379 - BWI76_RS04445 0.80 -0.6 918,454 + BWI76_RS04445 0.88 -0.9 918,527 - +1.4 918,741 + BWI76_RS04450 0.12 -0.1 918,749 - BWI76_RS04450 0.12 -0.5 918,914 + BWI76_RS04450 0.23 -0.6 918,914 + BWI76_RS04450 0.23 -1.0 918,938 + BWI76_RS04450 0.24 -0.4 919,454 + BWI76_RS04450 0.58 -1.6 919,509 + BWI76_RS04450 0.62 -3.2 919,529 - BWI76_RS04450 0.63 -0.3 919,639 + BWI76_RS04450 0.70 -2.4 920,000 - -0.6 920,081 - +0.0 920,159 - +0.3 920,251 + BWI76_RS04455 0.15 -2.0 920,281 - BWI76_RS04455 0.19 -1.0 920,283 + BWI76_RS04455 0.19 +0.2 920,350 + BWI76_RS04455 0.26 +1.4 920,372 - BWI76_RS04455 0.29 +0.4 920,479 - BWI76_RS04455 0.40 -0.4 920,497 - BWI76_RS04455 0.42 -0.5 920,689 + BWI76_RS04455 0.64 +2.9 920,728 + BWI76_RS04455 0.68 -0.5 920,873 + BWI76_RS04455 0.84 -0.8 920,952 + +1.0
Or see this region's nucleotide sequence