Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03080 and BWI76_RS03085 are separated by 151 nucleotides BWI76_RS03085 and BWI76_RS03090 are separated by 526 nucleotides
BWI76_RS03080: BWI76_RS03080 - (Fe-S)-cluster assembly protein, at 631,730 to 632,587
_RS03080
BWI76_RS03085: BWI76_RS03085 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) <small>(from data)</small>, at 632,739 to 634,652
_RS03085
BWI76_RS03090: BWI76_RS03090 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) <small>(from data)</small>, at 635,179 to 637,119
_RS03090
Position (kb) 632
633
634
635 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 631.852 kb on + strand, within BWI76_RS03080 at 631.860 kb on - strand, within BWI76_RS03080 at 631.932 kb on + strand, within BWI76_RS03080 at 632.081 kb on - strand, within BWI76_RS03080 at 632.086 kb on - strand, within BWI76_RS03080 at 632.231 kb on - strand, within BWI76_RS03080 at 632.591 kb on - strand at 632.629 kb on - strand at 632.758 kb on - strand at 632.771 kb on + strand at 632.771 kb on + strand at 632.771 kb on + strand at 632.839 kb on + strand at 632.928 kb on + strand at 632.928 kb on + strand at 633.035 kb on + strand, within BWI76_RS03085 at 633.035 kb on + strand, within BWI76_RS03085 at 633.281 kb on + strand, within BWI76_RS03085 at 633.286 kb on + strand, within BWI76_RS03085 at 633.289 kb on - strand, within BWI76_RS03085 at 633.832 kb on - strand, within BWI76_RS03085 at 633.899 kb on - strand, within BWI76_RS03085 at 633.982 kb on - strand, within BWI76_RS03085 at 634.063 kb on - strand, within BWI76_RS03085 at 634.108 kb on - strand, within BWI76_RS03085 at 634.214 kb on - strand, within BWI76_RS03085 at 634.301 kb on - strand, within BWI76_RS03085 at 634.422 kb on + strand, within BWI76_RS03085 at 634.430 kb on - strand, within BWI76_RS03085 at 634.430 kb on - strand, within BWI76_RS03085 at 634.562 kb on + strand at 634.573 kb on + strand at 634.979 kb on - strand at 634.997 kb on + strand at 634.997 kb on + strand at 635.118 kb on + strand at 635.226 kb on + strand at 635.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 631,852 + BWI76_RS03080 0.14 +1.8 631,860 - BWI76_RS03080 0.15 +0.8 631,932 + BWI76_RS03080 0.24 +0.6 632,081 - BWI76_RS03080 0.41 -0.0 632,086 - BWI76_RS03080 0.41 -0.1 632,231 - BWI76_RS03080 0.58 +1.3 632,591 - +0.6 632,629 - +1.3 632,758 - -0.2 632,771 + -0.3 632,771 + -1.4 632,771 + -0.8 632,839 + -0.3 632,928 + +0.7 632,928 + -0.7 633,035 + BWI76_RS03085 0.15 -3.4 633,035 + BWI76_RS03085 0.15 -0.8 633,281 + BWI76_RS03085 0.28 -0.6 633,286 + BWI76_RS03085 0.29 -0.7 633,289 - BWI76_RS03085 0.29 -2.4 633,832 - BWI76_RS03085 0.57 +0.1 633,899 - BWI76_RS03085 0.61 -0.5 633,982 - BWI76_RS03085 0.65 -0.6 634,063 - BWI76_RS03085 0.69 +0.2 634,108 - BWI76_RS03085 0.72 -0.9 634,214 - BWI76_RS03085 0.77 -1.4 634,301 - BWI76_RS03085 0.82 -0.9 634,422 + BWI76_RS03085 0.88 +0.6 634,430 - BWI76_RS03085 0.88 +0.2 634,430 - BWI76_RS03085 0.88 +0.4 634,562 + +0.4 634,573 + +0.3 634,979 - -0.6 634,997 + -0.8 634,997 + -0.4 635,118 + -0.2 635,226 + +0.8 635,325 - -1.6
Or see this region's nucleotide sequence