Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02220 and BWI76_RS02225 overlap by 4 nucleotides BWI76_RS02225 and BWI76_RS02230 are separated by 0 nucleotides BWI76_RS02230 and BWI76_RS02235 are separated by 204 nucleotides BWI76_RS02235 and BWI76_RS02240 are separated by 160 nucleotides
BWI76_RS02220: BWI76_RS02220 - carbon-phosphorus lyase subunit PhnH, at 459,421 to 460,005
_RS02220
BWI76_RS02225: BWI76_RS02225 - phosphonate C-P lyase system protein PhnG, at 460,002 to 460,454
_RS02225
BWI76_RS02230: BWI76_RS02230 - phosphonate metabolism transcriptional regulator PhnF, at 460,455 to 461,180
_RS02230
BWI76_RS02235: BWI76_RS02235 - VOC family protein, at 461,385 to 461,819
_RS02235
BWI76_RS02240: BWI76_RS02240 - alkylphosphonate utilization operon protein PhnA, at 461,980 to 462,315
_RS02240
Position (kb) 460
461
462 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 459.794 kb on + strand, within BWI76_RS02220 at 459.804 kb on + strand, within BWI76_RS02220 at 459.906 kb on + strand, within BWI76_RS02220 at 460.008 kb on - strand at 460.181 kb on - strand, within BWI76_RS02225 at 460.182 kb on - strand, within BWI76_RS02225 at 460.319 kb on + strand, within BWI76_RS02225 at 460.342 kb on + strand, within BWI76_RS02225 at 460.546 kb on - strand, within BWI76_RS02230 at 460.654 kb on - strand, within BWI76_RS02230 at 460.691 kb on + strand, within BWI76_RS02230 at 460.718 kb on + strand, within BWI76_RS02230 at 460.744 kb on + strand, within BWI76_RS02230 at 460.750 kb on - strand, within BWI76_RS02230 at 460.752 kb on - strand, within BWI76_RS02230 at 460.762 kb on - strand, within BWI76_RS02230 at 460.821 kb on + strand, within BWI76_RS02230 at 460.890 kb on - strand, within BWI76_RS02230 at 461.027 kb on + strand, within BWI76_RS02230 at 461.027 kb on + strand, within BWI76_RS02230 at 461.035 kb on - strand, within BWI76_RS02230 at 461.035 kb on - strand, within BWI76_RS02230 at 461.196 kb on + strand at 461.409 kb on - strand at 461.556 kb on + strand, within BWI76_RS02235 at 461.634 kb on + strand, within BWI76_RS02235 at 461.664 kb on - strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.751 kb on - strand, within BWI76_RS02235 at 461.774 kb on + strand, within BWI76_RS02235 at 461.777 kb on - strand at 461.800 kb on + strand at 461.809 kb on + strand at 461.815 kb on + strand at 461.824 kb on - strand at 461.828 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.853 kb on + strand at 461.853 kb on + strand at 461.853 kb on + strand at 461.854 kb on - strand at 461.861 kb on - strand at 461.861 kb on - strand at 461.895 kb on + strand at 461.895 kb on + strand at 461.900 kb on + strand at 461.901 kb on - strand at 461.908 kb on - strand at 461.914 kb on + strand at 461.919 kb on + strand at 461.919 kb on + strand at 461.921 kb on + strand at 461.921 kb on + strand at 461.927 kb on - strand at 462.021 kb on - strand, within BWI76_RS02240 at 462.040 kb on - strand, within BWI76_RS02240 at 462.055 kb on + strand at 462.056 kb on + strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.073 kb on + strand, within BWI76_RS02240 at 462.102 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.113 kb on + strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.144 kb on + strand, within BWI76_RS02240
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 459,794 + BWI76_RS02220 0.64 -0.7 459,804 + BWI76_RS02220 0.65 -0.0 459,906 + BWI76_RS02220 0.83 +0.6 460,008 - +0.2 460,181 - BWI76_RS02225 0.40 -0.8 460,182 - BWI76_RS02225 0.40 -0.1 460,319 + BWI76_RS02225 0.70 +0.4 460,342 + BWI76_RS02225 0.75 -0.1 460,546 - BWI76_RS02230 0.13 -0.4 460,654 - BWI76_RS02230 0.27 -1.8 460,691 + BWI76_RS02230 0.33 +0.6 460,718 + BWI76_RS02230 0.36 -0.8 460,744 + BWI76_RS02230 0.40 +0.4 460,750 - BWI76_RS02230 0.41 -1.2 460,752 - BWI76_RS02230 0.41 -2.6 460,762 - BWI76_RS02230 0.42 -0.3 460,821 + BWI76_RS02230 0.50 +0.3 460,890 - BWI76_RS02230 0.60 -0.4 461,027 + BWI76_RS02230 0.79 -1.5 461,027 + BWI76_RS02230 0.79 +0.0 461,035 - BWI76_RS02230 0.80 -0.3 461,035 - BWI76_RS02230 0.80 +0.1 461,196 + -1.3 461,409 - -0.2 461,556 + BWI76_RS02235 0.39 -0.3 461,634 + BWI76_RS02235 0.57 +0.4 461,664 - BWI76_RS02235 0.64 -0.3 461,738 + BWI76_RS02235 0.81 +1.0 461,738 + BWI76_RS02235 0.81 -0.6 461,738 + BWI76_RS02235 0.81 +0.3 461,746 - BWI76_RS02235 0.83 -0.0 461,746 - BWI76_RS02235 0.83 -2.6 461,746 - BWI76_RS02235 0.83 +0.1 461,746 - BWI76_RS02235 0.83 -0.4 461,751 - BWI76_RS02235 0.84 +0.1 461,774 + BWI76_RS02235 0.89 -0.1 461,777 - -1.4 461,800 + -0.3 461,809 + -0.4 461,815 + +0.7 461,824 - +2.1 461,828 - +0.1 461,830 - +0.5 461,830 - -1.6 461,830 - -1.1 461,830 - -0.6 461,853 + -0.0 461,853 + -0.6 461,853 + +3.1 461,854 - -1.6 461,861 - -1.1 461,861 - +1.4 461,895 + +0.5 461,895 + +0.0 461,900 + +0.5 461,901 - -0.6 461,908 - +0.6 461,914 + -0.3 461,919 + -0.1 461,919 + -2.0 461,921 + +0.4 461,921 + -0.3 461,927 - -0.2 462,021 - BWI76_RS02240 0.12 +0.4 462,040 - BWI76_RS02240 0.18 -0.1 462,055 + +3.6 462,056 + BWI76_RS02240 0.23 +0.6 462,064 - BWI76_RS02240 0.25 +0.2 462,064 - BWI76_RS02240 0.25 +0.8 462,064 - BWI76_RS02240 0.25 -0.1 462,064 - BWI76_RS02240 0.25 +0.3 462,064 - BWI76_RS02240 0.25 +0.2 462,064 - BWI76_RS02240 0.25 -1.9 462,064 - BWI76_RS02240 0.25 +1.1 462,073 + BWI76_RS02240 0.28 -0.3 462,102 + BWI76_RS02240 0.36 +0.3 462,112 + BWI76_RS02240 0.39 -0.4 462,112 + BWI76_RS02240 0.39 -1.9 462,113 + BWI76_RS02240 0.40 -3.5 462,120 - BWI76_RS02240 0.42 -0.2 462,120 - BWI76_RS02240 0.42 +0.5 462,120 - BWI76_RS02240 0.42 -0.1 462,121 - BWI76_RS02240 0.42 -0.5 462,121 - BWI76_RS02240 0.42 +0.8 462,144 + BWI76_RS02240 0.49 +1.2
Or see this region's nucleotide sequence