Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02015

Experiment: K. M5a1 KLEB23 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02005 and BWI76_RS02010 are separated by 36 nucleotidesBWI76_RS02010 and BWI76_RS02015 are separated by 151 nucleotidesBWI76_RS02015 and BWI76_RS02020 are separated by 146 nucleotides BWI76_RS02005: BWI76_RS02005 - glutathione S-transferase, at 411,855 to 412,523 _RS02005 BWI76_RS02010: BWI76_RS02010 - hypothetical protein, at 412,560 to 412,760 _RS02010 BWI76_RS02015: BWI76_RS02015 - NCS2 family permease, at 412,912 to 414,261 _RS02015 BWI76_RS02020: BWI76_RS02020 - Na+/H+ antiporter, at 414,408 to 416,054 _RS02020 Position (kb) 412 413 414 415Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 411.943 kb on - strand, within BWI76_RS02005at 411.951 kb on - strand, within BWI76_RS02005at 411.953 kb on + strand, within BWI76_RS02005at 411.959 kb on + strand, within BWI76_RS02005at 412.056 kb on + strand, within BWI76_RS02005at 412.078 kb on + strand, within BWI76_RS02005at 412.085 kb on - strand, within BWI76_RS02005at 412.145 kb on + strand, within BWI76_RS02005at 412.150 kb on + strand, within BWI76_RS02005at 412.213 kb on - strand, within BWI76_RS02005at 412.253 kb on + strand, within BWI76_RS02005at 412.261 kb on - strand, within BWI76_RS02005at 412.285 kb on - strand, within BWI76_RS02005at 412.322 kb on + strand, within BWI76_RS02005at 412.461 kb on - strandat 412.463 kb on + strandat 412.863 kb on - strandat 413.015 kb on + strandat 413.016 kb on - strandat 413.130 kb on + strand, within BWI76_RS02015at 413.138 kb on - strand, within BWI76_RS02015at 413.390 kb on + strand, within BWI76_RS02015at 413.769 kb on + strandat 413.813 kb on - strand, within BWI76_RS02015at 413.874 kb on - strand, within BWI76_RS02015at 413.935 kb on - strand, within BWI76_RS02015at 414.012 kb on - strand, within BWI76_RS02015at 414.098 kb on + strand, within BWI76_RS02015at 414.143 kb on + strandat 414.151 kb on - strandat 414.191 kb on + strandat 414.199 kb on - strandat 414.201 kb on + strandat 414.209 kb on - strandat 414.209 kb on - strandat 414.335 kb on + strandat 414.347 kb on + strandat 414.439 kb on - strandat 414.439 kb on - strandat 414.507 kb on - strandat 414.525 kb on + strandat 414.528 kb on - strandat 414.530 kb on + strandat 414.533 kb on - strandat 414.565 kb on + strandat 414.602 kb on + strand, within BWI76_RS02020at 414.727 kb on - strand, within BWI76_RS02020at 414.798 kb on - strand, within BWI76_RS02020at 414.808 kb on - strand, within BWI76_RS02020at 414.808 kb on - strand, within BWI76_RS02020at 415.019 kb on - strand, within BWI76_RS02020at 415.074 kb on + strand, within BWI76_RS02020at 415.099 kb on + strand, within BWI76_RS02020at 415.099 kb on + strand, within BWI76_RS02020at 415.107 kb on - strand, within BWI76_RS02020at 415.166 kb on - strand, within BWI76_RS02020

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1
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411,943 - BWI76_RS02005 0.13 -0.2
411,951 - BWI76_RS02005 0.14 -0.7
411,953 + BWI76_RS02005 0.15 -2.8
411,959 + BWI76_RS02005 0.16 +0.1
412,056 + BWI76_RS02005 0.30 +0.1
412,078 + BWI76_RS02005 0.33 +1.3
412,085 - BWI76_RS02005 0.34 +0.8
412,145 + BWI76_RS02005 0.43 +1.0
412,150 + BWI76_RS02005 0.44 +0.6
412,213 - BWI76_RS02005 0.54 -1.0
412,253 + BWI76_RS02005 0.59 +0.2
412,261 - BWI76_RS02005 0.61 -0.9
412,285 - BWI76_RS02005 0.64 -0.9
412,322 + BWI76_RS02005 0.70 -2.2
412,461 - -0.1
412,463 + +0.9
412,863 - +0.1
413,015 + -0.4
413,016 - +0.9
413,130 + BWI76_RS02015 0.16 +0.0
413,138 - BWI76_RS02015 0.17 +0.0
413,390 + BWI76_RS02015 0.35 +0.9
413,769 + +1.3
413,813 - BWI76_RS02015 0.67 +0.5
413,874 - BWI76_RS02015 0.71 +0.4
413,935 - BWI76_RS02015 0.76 -0.2
414,012 - BWI76_RS02015 0.81 +0.3
414,098 + BWI76_RS02015 0.88 -0.0
414,143 + +0.1
414,151 - +0.3
414,191 + +0.1
414,199 - -1.2
414,201 + +1.2
414,209 - -0.0
414,209 - +0.7
414,335 + -0.2
414,347 + +0.6
414,439 - +0.8
414,439 - +0.2
414,507 - +2.1
414,525 + +0.5
414,528 - +0.2
414,530 + -0.2
414,533 - -0.0
414,565 + +1.3
414,602 + BWI76_RS02020 0.12 -0.7
414,727 - BWI76_RS02020 0.19 -0.5
414,798 - BWI76_RS02020 0.24 +0.4
414,808 - BWI76_RS02020 0.24 -1.4
414,808 - BWI76_RS02020 0.24 +0.3
415,019 - BWI76_RS02020 0.37 +0.7
415,074 + BWI76_RS02020 0.40 -0.0
415,099 + BWI76_RS02020 0.42 -1.1
415,099 + BWI76_RS02020 0.42 -0.3
415,107 - BWI76_RS02020 0.42 -0.2
415,166 - BWI76_RS02020 0.46 -0.6

Or see this region's nucleotide sequence