Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22765 and BWI76_RS22770 are separated by 346 nucleotides BWI76_RS22770 and BWI76_RS22775 overlap by 72 nucleotides BWI76_RS22775 and BWI76_RS22780 are separated by 56 nucleotides BWI76_RS22780 and BWI76_RS22785 are separated by 459 nucleotides
BWI76_RS22765: BWI76_RS22765 - sulfite reductase subunit alpha, at 4,745,693 to 4,747,492
_RS22765
BWI76_RS22770: BWI76_RS22770 - 6-pyruvoyl tetrahydrobiopterin synthase, at 4,747,839 to 4,748,000
_RS22770
BWI76_RS22775: BWI76_RS22775 - 6-carboxytetrahydropterin synthase QueD, at 4,747,929 to 4,748,291
_RS22775
BWI76_RS22780: BWI76_RS22780 - 7-carboxy-7-deazaguanine synthase QueE, at 4,748,348 to 4,749,019
_RS22780
BWI76_RS22785: BWI76_RS22785 - NAD(P)H-binding protein, at 4,749,479 to 4,750,588
_RS22785
Position (kb)
4748
4749
4750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4747.376 kb on - strand at 4747.376 kb on - strand at 4747.498 kb on + strand at 4747.519 kb on - strand at 4747.612 kb on + strand at 4747.612 kb on + strand at 4747.612 kb on + strand at 4747.613 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.677 kb on + strand at 4747.692 kb on - strand at 4747.943 kb on + strand at 4747.943 kb on + strand at 4747.943 kb on + strand at 4747.957 kb on + strand at 4747.968 kb on + strand at 4748.026 kb on + strand, within BWI76_RS22775 at 4748.096 kb on + strand, within BWI76_RS22775 at 4748.096 kb on + strand, within BWI76_RS22775 at 4748.165 kb on + strand, within BWI76_RS22775 at 4748.187 kb on - strand, within BWI76_RS22775 at 4748.330 kb on - strand at 4748.401 kb on - strand at 4748.468 kb on - strand, within BWI76_RS22780 at 4748.605 kb on + strand, within BWI76_RS22780 at 4748.613 kb on - strand, within BWI76_RS22780 at 4748.627 kb on + strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.774 kb on - strand, within BWI76_RS22780 at 4748.779 kb on - strand, within BWI76_RS22780 at 4748.833 kb on + strand, within BWI76_RS22780 at 4748.884 kb on + strand, within BWI76_RS22780 at 4748.972 kb on + strand at 4748.974 kb on - strand at 4748.987 kb on + strand at 4748.988 kb on + strand at 4748.996 kb on - strand at 4749.014 kb on - strand at 4749.030 kb on + strand at 4749.068 kb on + strand at 4749.073 kb on + strand at 4749.073 kb on + strand at 4749.079 kb on + strand at 4749.081 kb on - strand at 4749.082 kb on - strand at 4749.084 kb on + strand at 4749.118 kb on + strand at 4749.126 kb on - strand at 4749.128 kb on + strand at 4749.134 kb on - strand at 4749.134 kb on - strand at 4749.156 kb on - strand at 4749.196 kb on + strand at 4749.204 kb on - strand at 4749.204 kb on - strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.305 kb on - strand at 4749.305 kb on - strand at 4749.330 kb on + strand at 4749.350 kb on - strand at 4749.389 kb on - strand at 4749.459 kb on + strand at 4749.467 kb on - strand at 4749.473 kb on + strand at 4749.517 kb on + strand at 4749.517 kb on + strand at 4749.517 kb on + strand at 4749.522 kb on + strand at 4749.522 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.535 kb on - strand at 4749.535 kb on - strand at 4749.572 kb on + strand at 4749.572 kb on - strand at 4749.572 kb on - strand at 4749.574 kb on - strand at 4749.580 kb on - strand at 4749.580 kb on - strand at 4749.587 kb on + strand at 4749.594 kb on + strand, within BWI76_RS22785 at 4749.608 kb on + strand, within BWI76_RS22785 at 4749.612 kb on + strand, within BWI76_RS22785 at 4749.613 kb on + strand, within BWI76_RS22785 at 4749.613 kb on + strand, within BWI76_RS22785 at 4749.619 kb on + strand, within BWI76_RS22785 at 4749.619 kb on + strand, within BWI76_RS22785 at 4749.626 kb on - strand, within BWI76_RS22785 at 4749.626 kb on - strand, within BWI76_RS22785 at 4749.662 kb on + strand, within BWI76_RS22785 at 4749.662 kb on + strand, within BWI76_RS22785 at 4749.664 kb on + strand, within BWI76_RS22785 at 4749.675 kb on + strand, within BWI76_RS22785 at 4749.679 kb on - strand, within BWI76_RS22785 at 4749.681 kb on - strand, within BWI76_RS22785 at 4749.686 kb on - strand, within BWI76_RS22785 at 4749.691 kb on - strand, within BWI76_RS22785 at 4749.752 kb on - strand, within BWI76_RS22785 at 4749.757 kb on - strand, within BWI76_RS22785 at 4749.847 kb on + strand, within BWI76_RS22785 at 4749.897 kb on - strand, within BWI76_RS22785 at 4749.897 kb on - strand, within BWI76_RS22785
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 4,747,376 - +0.5 4,747,376 - +0.5 4,747,498 + -1.3 4,747,519 - +0.7 4,747,612 + -3.1 4,747,612 + +0.5 4,747,612 + +0.1 4,747,613 - +0.7 4,747,620 - -0.8 4,747,620 - -0.5 4,747,620 - -0.3 4,747,620 - -0.5 4,747,677 + +0.0 4,747,692 - +0.2 4,747,943 + +0.1 4,747,943 + -0.2 4,747,943 + -1.0 4,747,957 + -0.3 4,747,968 + +0.1 4,748,026 + BWI76_RS22775 0.27 +0.6 4,748,096 + BWI76_RS22775 0.46 -0.3 4,748,096 + BWI76_RS22775 0.46 +0.4 4,748,165 + BWI76_RS22775 0.65 -0.5 4,748,187 - BWI76_RS22775 0.71 +0.5 4,748,330 - -0.4 4,748,401 - +0.1 4,748,468 - BWI76_RS22780 0.18 -1.8 4,748,605 + BWI76_RS22780 0.38 -0.7 4,748,613 - BWI76_RS22780 0.39 -1.0 4,748,627 + BWI76_RS22780 0.42 +0.8 4,748,635 - BWI76_RS22780 0.43 -0.7 4,748,635 - BWI76_RS22780 0.43 +0.8 4,748,774 - BWI76_RS22780 0.63 +0.7 4,748,779 - BWI76_RS22780 0.64 -2.3 4,748,833 + BWI76_RS22780 0.72 -0.3 4,748,884 + BWI76_RS22780 0.80 +0.7 4,748,972 + -0.1 4,748,974 - +0.4 4,748,987 + +0.4 4,748,988 + -0.0 4,748,996 - -1.6 4,749,014 - +1.4 4,749,030 + -1.4 4,749,068 + -1.0 4,749,073 + +1.0 4,749,073 + +0.7 4,749,079 + -1.8 4,749,081 - -0.3 4,749,082 - -0.5 4,749,084 + -1.4 4,749,118 + +0.1 4,749,126 - -1.1 4,749,128 + +0.2 4,749,134 - +0.1 4,749,134 - -2.1 4,749,156 - +0.2 4,749,196 + -1.6 4,749,204 - -3.2 4,749,204 - -1.1 4,749,238 + -0.2 4,749,238 + +1.5 4,749,238 + -1.6 4,749,246 - -0.5 4,749,246 - +0.4 4,749,246 - +0.0 4,749,246 - +1.8 4,749,305 - +0.0 4,749,305 - +1.5 4,749,330 + -0.8 4,749,350 - +0.4 4,749,389 - +0.2 4,749,459 + +0.2 4,749,467 - +1.0 4,749,473 + -0.4 4,749,517 + +0.2 4,749,517 + -2.3 4,749,517 + +1.7 4,749,522 + -2.1 4,749,522 + +0.2 4,749,527 + +0.8 4,749,527 + +0.0 4,749,527 + -0.8 4,749,527 + +0.5 4,749,527 + +0.2 4,749,527 + -0.7 4,749,527 + -0.8 4,749,535 - -1.1 4,749,535 - +1.3 4,749,572 + +0.1 4,749,572 - -2.2 4,749,572 - +0.9 4,749,574 - -0.0 4,749,580 - -2.4 4,749,580 - +0.7 4,749,587 + -0.8 4,749,594 + BWI76_RS22785 0.10 -0.3 4,749,608 + BWI76_RS22785 0.12 -1.5 4,749,612 + BWI76_RS22785 0.12 -2.6 4,749,613 + BWI76_RS22785 0.12 -0.0 4,749,613 + BWI76_RS22785 0.12 +0.2 4,749,619 + BWI76_RS22785 0.13 -0.5 4,749,619 + BWI76_RS22785 0.13 -1.0 4,749,626 - BWI76_RS22785 0.13 +2.5 4,749,626 - BWI76_RS22785 0.13 -3.0 4,749,662 + BWI76_RS22785 0.16 -0.1 4,749,662 + BWI76_RS22785 0.16 -0.7 4,749,664 + BWI76_RS22785 0.17 -0.8 4,749,675 + BWI76_RS22785 0.18 +0.0 4,749,679 - BWI76_RS22785 0.18 -0.5 4,749,681 - BWI76_RS22785 0.18 -2.3 4,749,686 - BWI76_RS22785 0.19 +0.8 4,749,691 - BWI76_RS22785 0.19 +0.6 4,749,752 - BWI76_RS22785 0.25 -0.9 4,749,757 - BWI76_RS22785 0.25 -1.4 4,749,847 + BWI76_RS22785 0.33 +0.2 4,749,897 - BWI76_RS22785 0.38 -1.8 4,749,897 - BWI76_RS22785 0.38 +0.1
Or see this region's nucleotide sequence