Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22340 and BWI76_RS22345 are separated by 208 nucleotides BWI76_RS22345 and BWI76_RS22350 are separated by 3 nucleotides BWI76_RS22350 and BWI76_RS22355 overlap by 10 nucleotides BWI76_RS22355 and BWI76_RS22360 are separated by 0 nucleotides
BWI76_RS22340: BWI76_RS22340 - formate hydrogenlyase regulatory protein HycA, at 4,657,318 to 4,657,767
_RS22340
BWI76_RS22345: BWI76_RS22345 - hydrogenase maturation nickel metallochaperone HypA, at 4,657,976 to 4,658,326
_RS22345
BWI76_RS22350: BWI76_RS22350 - hydrogenase nickel incorporation protein HypB, at 4,658,330 to 4,659,202
_RS22350
BWI76_RS22355: BWI76_RS22355 - hydrogenase assembly chaperone, at 4,659,193 to 4,659,465
_RS22355
BWI76_RS22360: BWI76_RS22360 - hydrogenase formation protein HypD, at 4,659,466 to 4,660,587
_RS22360
Position (kb)
4658
4659
4660 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4657.609 kb on - strand, within BWI76_RS22340 at 4657.659 kb on + strand, within BWI76_RS22340 at 4657.659 kb on + strand, within BWI76_RS22340 at 4657.667 kb on - strand, within BWI76_RS22340 at 4657.671 kb on + strand, within BWI76_RS22340 at 4657.783 kb on + strand at 4657.783 kb on + strand at 4657.989 kb on + strand at 4657.989 kb on + strand at 4657.997 kb on - strand at 4658.045 kb on + strand, within BWI76_RS22345 at 4658.102 kb on + strand, within BWI76_RS22345 at 4658.151 kb on + strand, within BWI76_RS22345 at 4658.161 kb on + strand, within BWI76_RS22345 at 4658.174 kb on - strand, within BWI76_RS22345 at 4658.259 kb on - strand, within BWI76_RS22345 at 4658.426 kb on + strand, within BWI76_RS22350 at 4658.470 kb on + strand, within BWI76_RS22350 at 4658.478 kb on - strand, within BWI76_RS22350 at 4658.745 kb on - strand, within BWI76_RS22350 at 4658.745 kb on - strand, within BWI76_RS22350 at 4658.950 kb on + strand, within BWI76_RS22350 at 4659.156 kb on + strand at 4659.163 kb on - strand at 4659.254 kb on - strand, within BWI76_RS22355 at 4659.334 kb on + strand, within BWI76_RS22355 at 4659.936 kb on - strand, within BWI76_RS22360 at 4660.068 kb on + strand, within BWI76_RS22360 at 4660.101 kb on - strand, within BWI76_RS22360 at 4660.135 kb on - strand, within BWI76_RS22360 at 4660.196 kb on - strand, within BWI76_RS22360
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 4,657,609 - BWI76_RS22340 0.65 -0.2 4,657,659 + BWI76_RS22340 0.76 +1.0 4,657,659 + BWI76_RS22340 0.76 +0.5 4,657,667 - BWI76_RS22340 0.78 +1.2 4,657,671 + BWI76_RS22340 0.78 +0.0 4,657,783 + -0.6 4,657,783 + -0.6 4,657,989 + +1.7 4,657,989 + -3.2 4,657,997 - +0.9 4,658,045 + BWI76_RS22345 0.20 -1.7 4,658,102 + BWI76_RS22345 0.36 +0.1 4,658,151 + BWI76_RS22345 0.50 +0.2 4,658,161 + BWI76_RS22345 0.53 +0.0 4,658,174 - BWI76_RS22345 0.56 +0.5 4,658,259 - BWI76_RS22345 0.81 +0.3 4,658,426 + BWI76_RS22350 0.11 -1.3 4,658,470 + BWI76_RS22350 0.16 -1.1 4,658,478 - BWI76_RS22350 0.17 +0.8 4,658,745 - BWI76_RS22350 0.48 -2.6 4,658,745 - BWI76_RS22350 0.48 -0.8 4,658,950 + BWI76_RS22350 0.71 -1.7 4,659,156 + -1.4 4,659,163 - +1.5 4,659,254 - BWI76_RS22355 0.22 +0.8 4,659,334 + BWI76_RS22355 0.52 +1.1 4,659,936 - BWI76_RS22360 0.42 +0.3 4,660,068 + BWI76_RS22360 0.54 -0.4 4,660,101 - BWI76_RS22360 0.57 +0.1 4,660,135 - BWI76_RS22360 0.60 +0.3 4,660,196 - BWI76_RS22360 0.65 +0.3
Or see this region's nucleotide sequence