Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16430 and BWI76_RS16435 are separated by 41 nucleotides BWI76_RS16435 and BWI76_RS16440 are separated by 171 nucleotides BWI76_RS16440 and BWI76_RS16445 are separated by 10 nucleotides BWI76_RS16445 and BWI76_RS16450 overlap by 1 nucleotides
BWI76_RS16430: BWI76_RS16430 - GGDEF domain-containing protein, at 3,408,369 to 3,409,721
_RS16430
BWI76_RS16435: BWI76_RS16435 - SlyA family transcriptional regulator, at 3,409,763 to 3,410,203
_RS16435
BWI76_RS16440: BWI76_RS16440 - DUF1656 domain-containing protein, at 3,410,375 to 3,410,611
_RS16440
BWI76_RS16445: BWI76_RS16445 - auxiliary transporter membrane fusion protein, at 3,410,622 to 3,411,518
_RS16445
BWI76_RS16450: BWI76_RS16450 - fusaric acid resistance protein, at 3,411,518 to 3,413,542
_RS16450
Position (kb)
3410
3411
3412 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3409.719 kb on - strand at 3410.839 kb on - strand, within BWI76_RS16445 at 3411.160 kb on + strand, within BWI76_RS16445 at 3411.200 kb on - strand, within BWI76_RS16445 at 3411.515 kb on + strand at 3411.893 kb on + strand, within BWI76_RS16450 at 3411.893 kb on + strand, within BWI76_RS16450 at 3411.893 kb on + strand, within BWI76_RS16450 at 3411.901 kb on - strand, within BWI76_RS16450 at 3411.901 kb on - strand, within BWI76_RS16450 at 3412.190 kb on + strand, within BWI76_RS16450 at 3412.348 kb on - strand, within BWI76_RS16450
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,409,719 - -2.9 3,410,839 - BWI76_RS16445 0.24 -0.1 3,411,160 + BWI76_RS16445 0.60 -1.6 3,411,200 - BWI76_RS16445 0.64 -0.6 3,411,515 + -0.6 3,411,893 + BWI76_RS16450 0.19 +3.4 3,411,893 + BWI76_RS16450 0.19 +0.4 3,411,893 + BWI76_RS16450 0.19 +1.3 3,411,901 - BWI76_RS16450 0.19 -0.3 3,411,901 - BWI76_RS16450 0.19 +0.5 3,412,190 + BWI76_RS16450 0.33 -0.7 3,412,348 - BWI76_RS16450 0.41 +0.1
Or see this region's nucleotide sequence