Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16020 and BWI76_RS16025 overlap by 1 nucleotides BWI76_RS16025 and BWI76_RS16030 are separated by 17 nucleotides BWI76_RS16030 and BWI76_RS16035 overlap by 8 nucleotides
BWI76_RS16020: BWI76_RS16020 - malonate decarboxylase subunit alpha, at 3,322,117 to 3,323,772
_RS16020
BWI76_RS16025: BWI76_RS16025 - triphosphoribosyl-dephospho-CoA synthase, at 3,323,772 to 3,324,614
_RS16025
BWI76_RS16030: BWI76_RS16030 - malonate decarboxylase acyl carrier protein, at 3,324,632 to 3,324,931
_RS16030
BWI76_RS16035: BWI76_RS16035 - biotin-independent malonate decarboxylase subunit beta, at 3,324,924 to 3,325,757
_RS16035
Position (kb)
3323
3324
3325 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3322.910 kb on + strand, within BWI76_RS16020 at 3322.965 kb on - strand, within BWI76_RS16020 at 3323.076 kb on - strand, within BWI76_RS16020 at 3323.385 kb on - strand, within BWI76_RS16020 at 3323.525 kb on - strand, within BWI76_RS16020 at 3323.737 kb on + strand at 3324.104 kb on - strand, within BWI76_RS16025 at 3324.304 kb on - strand, within BWI76_RS16025 at 3324.729 kb on - strand, within BWI76_RS16030 at 3324.776 kb on - strand, within BWI76_RS16030 at 3324.906 kb on + strand at 3324.991 kb on - strand at 3325.238 kb on + strand, within BWI76_RS16035 at 3325.287 kb on - strand, within BWI76_RS16035 at 3325.339 kb on + strand, within BWI76_RS16035
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,322,910 + BWI76_RS16020 0.48 -0.4 3,322,965 - BWI76_RS16020 0.51 +2.0 3,323,076 - BWI76_RS16020 0.58 +0.9 3,323,385 - BWI76_RS16020 0.77 -0.2 3,323,525 - BWI76_RS16020 0.85 +0.2 3,323,737 + -0.3 3,324,104 - BWI76_RS16025 0.39 -1.3 3,324,304 - BWI76_RS16025 0.63 -2.0 3,324,729 - BWI76_RS16030 0.32 -1.6 3,324,776 - BWI76_RS16030 0.48 -1.0 3,324,906 + -1.0 3,324,991 - -1.9 3,325,238 + BWI76_RS16035 0.38 +0.4 3,325,287 - BWI76_RS16035 0.44 +0.1 3,325,339 + BWI76_RS16035 0.50 +0.4
Or see this region's nucleotide sequence