Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15175

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15170 and BWI76_RS15175 are separated by 244 nucleotidesBWI76_RS15175 and BWI76_RS15180 are separated by 305 nucleotides BWI76_RS15170: BWI76_RS15170 - trans-aconitate 2-methyltransferase, at 3,180,191 to 3,180,943 _RS15170 BWI76_RS15175: BWI76_RS15175 - N-acetyltransferase, at 3,181,188 to 3,181,628 _RS15175 BWI76_RS15180: BWI76_RS15180 - altronate oxidoreductase, at 3,181,934 to 3,183,385 _RS15180 Position (kb) 3181 3182Strain fitness (log2 ratio) -3 -2 -1 0 1at 3180.257 kb on - strandat 3180.758 kb on + strand, within BWI76_RS15170at 3181.022 kb on + strandat 3181.071 kb on - strandat 3181.278 kb on + strand, within BWI76_RS15175at 3181.295 kb on - strand, within BWI76_RS15175at 3181.487 kb on - strand, within BWI76_RS15175at 3181.513 kb on - strand, within BWI76_RS15175at 3181.582 kb on - strand, within BWI76_RS15175at 3181.625 kb on + strandat 3181.764 kb on - strandat 3181.771 kb on - strandat 3182.136 kb on - strand, within BWI76_RS15180at 3182.157 kb on + strand, within BWI76_RS15180at 3182.285 kb on + strand, within BWI76_RS15180at 3182.293 kb on - strand, within BWI76_RS15180at 3182.480 kb on + strand, within BWI76_RS15180

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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3,180,257 - +1.1
3,180,758 + BWI76_RS15170 0.75 -1.7
3,181,022 + -1.1
3,181,071 - +0.1
3,181,278 + BWI76_RS15175 0.20 -0.1
3,181,295 - BWI76_RS15175 0.24 -1.4
3,181,487 - BWI76_RS15175 0.68 -1.1
3,181,513 - BWI76_RS15175 0.74 -2.1
3,181,582 - BWI76_RS15175 0.89 -0.2
3,181,625 + +1.5
3,181,764 - -2.1
3,181,771 - +1.3
3,182,136 - BWI76_RS15180 0.14 -2.9
3,182,157 + BWI76_RS15180 0.15 +0.9
3,182,285 + BWI76_RS15180 0.24 -1.6
3,182,293 - BWI76_RS15180 0.25 -0.7
3,182,480 + BWI76_RS15180 0.38 -3.1

Or see this region's nucleotide sequence