Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14920 and BWI76_RS14925 overlap by 4 nucleotides BWI76_RS14925 and BWI76_RS14930 are separated by 24 nucleotides BWI76_RS14930 and BWI76_RS14935 are separated by 288 nucleotides BWI76_RS14935 and BWI76_RS14940 are separated by 139 nucleotides
BWI76_RS14920: BWI76_RS14920 - ABC transporter permease, at 3,127,403 to 3,128,410
_RS14920
BWI76_RS14925: BWI76_RS14925 - ABC transporter substrate-binding protein, at 3,128,407 to 3,129,363
_RS14925
BWI76_RS14930: BWI76_RS14930 - hypothetical protein, at 3,129,388 to 3,130,197
_RS14930
BWI76_RS14935: BWI76_RS14935 - NIPSNAP family containing protein, at 3,130,486 to 3,130,815
_RS14935
BWI76_RS14940: BWI76_RS14940 - hypothetical protein, at 3,130,955 to 3,131,803
_RS14940
Position (kb)
3129
3130
3131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3128.672 kb on - strand, within BWI76_RS14925 at 3128.743 kb on + strand, within BWI76_RS14925 at 3128.910 kb on - strand, within BWI76_RS14925 at 3128.915 kb on - strand, within BWI76_RS14925 at 3129.255 kb on + strand, within BWI76_RS14925 at 3129.288 kb on - strand at 3129.397 kb on + strand at 3129.607 kb on + strand, within BWI76_RS14930 at 3130.446 kb on - strand at 3130.734 kb on - strand, within BWI76_RS14935 at 3130.933 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,128,672 - BWI76_RS14925 0.28 +0.7 3,128,743 + BWI76_RS14925 0.35 +1.3 3,128,910 - BWI76_RS14925 0.53 -0.2 3,128,915 - BWI76_RS14925 0.53 -0.3 3,129,255 + BWI76_RS14925 0.89 +0.6 3,129,288 - +0.4 3,129,397 + -3.5 3,129,607 + BWI76_RS14930 0.27 -1.0 3,130,446 - -0.5 3,130,734 - BWI76_RS14935 0.75 -0.6 3,130,933 + +1.8
Or see this region's nucleotide sequence