Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14780

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14770 and BWI76_RS14775 overlap by 8 nucleotidesBWI76_RS14775 and BWI76_RS14780 are separated by 9 nucleotidesBWI76_RS14780 and BWI76_RS14785 are separated by 435 nucleotidesBWI76_RS14785 and BWI76_RS14790 are separated by 36 nucleotides BWI76_RS14770: BWI76_RS14770 - PTS IIA 2 domain protein, at 3,098,331 to 3,098,774 _RS14770 BWI76_RS14775: BWI76_RS14775 - creatinine amidohydrolase, at 3,098,767 to 3,099,507 _RS14775 BWI76_RS14780: BWI76_RS14780 - phosphotriesterase, at 3,099,517 to 3,100,488 _RS14780 BWI76_RS14785: BWI76_RS14785 - hypothetical protein, at 3,100,924 to 3,101,172 _RS14785 BWI76_RS14790: BWI76_RS14790 - 2-dehydropantoate 2-reductase, at 3,101,209 to 3,102,102 _RS14790 Position (kb) 3099 3100 3101Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3098.731 kb on - strandat 3098.906 kb on + strand, within BWI76_RS14775at 3099.126 kb on + strandat 3099.191 kb on + strand, within BWI76_RS14775at 3099.743 kb on + strand, within BWI76_RS14780at 3100.077 kb on + strand, within BWI76_RS14780at 3100.176 kb on - strand, within BWI76_RS14780at 3100.627 kb on + strandat 3101.321 kb on - strandat 3101.463 kb on + strand, within BWI76_RS14790

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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3,098,731 - -2.6
3,098,906 + BWI76_RS14775 0.19 -2.0
3,099,126 + +3.9
3,099,191 + BWI76_RS14775 0.57 +0.2
3,099,743 + BWI76_RS14780 0.23 -1.3
3,100,077 + BWI76_RS14780 0.58 -0.2
3,100,176 - BWI76_RS14780 0.68 +0.0
3,100,627 + -0.1
3,101,321 - +0.5
3,101,463 + BWI76_RS14790 0.28 +2.5

Or see this region's nucleotide sequence