Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14270 and BWI76_RS14275 are separated by 387 nucleotides BWI76_RS14275 and BWI76_RS14280 are separated by 74 nucleotides BWI76_RS14280 and BWI76_RS14285 are separated by 310 nucleotides BWI76_RS14285 and BWI76_RS14290 are separated by 42 nucleotides
BWI76_RS14270: BWI76_RS14270 - transporter, at 2,984,393 to 2,985,205
_RS14270
BWI76_RS14275: BWI76_RS14275 - hypothetical protein, at 2,985,593 to 2,986,789
_RS14275
BWI76_RS14280: BWI76_RS14280 - hypothetical protein, at 2,986,864 to 2,987,121
_RS14280
BWI76_RS14285: BWI76_RS14285 - IS3 family transposase, at 2,987,432 to 2,987,722
_RS14285
BWI76_RS14290: BWI76_RS14290 - AAA family ATPase, at 2,987,765 to 2,988,368
_RS14290
Position (kb)
2985
2986
2987 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2984.642 kb on - strand, within BWI76_RS14270 at 2984.673 kb on + strand, within BWI76_RS14270 at 2984.673 kb on + strand, within BWI76_RS14270 at 2984.775 kb on + strand, within BWI76_RS14270 at 2984.835 kb on - strand at 2984.938 kb on + strand, within BWI76_RS14270 at 2985.009 kb on + strand, within BWI76_RS14270 at 2985.033 kb on + strand, within BWI76_RS14270 at 2985.267 kb on + strand at 2985.269 kb on + strand at 2985.269 kb on + strand at 2985.270 kb on - strand at 2985.277 kb on - strand at 2985.386 kb on + strand at 2985.423 kb on - strand at 2985.449 kb on + strand at 2985.449 kb on + strand at 2985.449 kb on + strand at 2985.449 kb on + strand at 2985.449 kb on + strand at 2985.450 kb on + strand at 2985.457 kb on - strand at 2985.457 kb on - strand at 2985.465 kb on - strand at 2985.495 kb on + strand at 2985.495 kb on + strand at 2985.495 kb on + strand at 2985.495 kb on + strand at 2985.495 kb on + strand at 2985.495 kb on + strand at 2985.501 kb on + strand at 2985.503 kb on + strand at 2985.514 kb on + strand at 2985.522 kb on - strand at 2985.661 kb on + strand at 2985.707 kb on - strand at 2985.748 kb on - strand, within BWI76_RS14275 at 2985.754 kb on - strand, within BWI76_RS14275 at 2985.868 kb on + strand, within BWI76_RS14275 at 2985.882 kb on - strand, within BWI76_RS14275 at 2986.088 kb on + strand, within BWI76_RS14275 at 2986.212 kb on + strand, within BWI76_RS14275 at 2986.276 kb on + strand, within BWI76_RS14275 at 2986.281 kb on + strand, within BWI76_RS14275 at 2986.299 kb on - strand, within BWI76_RS14275 at 2986.310 kb on + strand, within BWI76_RS14275 at 2986.351 kb on + strand, within BWI76_RS14275 at 2986.439 kb on - strand, within BWI76_RS14275 at 2986.457 kb on - strand, within BWI76_RS14275 at 2986.542 kb on - strand, within BWI76_RS14275 at 2986.613 kb on - strand, within BWI76_RS14275 at 2986.614 kb on + strand, within BWI76_RS14275 at 2986.839 kb on - strand at 2986.875 kb on + strand at 2986.942 kb on + strand, within BWI76_RS14280 at 2986.993 kb on + strand, within BWI76_RS14280 at 2987.043 kb on - strand, within BWI76_RS14280 at 2987.173 kb on + strand at 2987.185 kb on - strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.250 kb on - strand at 2987.275 kb on + strand at 2987.276 kb on + strand at 2987.293 kb on - strand at 2987.401 kb on + strand at 2987.446 kb on + strand at 2987.481 kb on - strand, within BWI76_RS14285 at 2987.491 kb on - strand, within BWI76_RS14285 at 2987.514 kb on - strand, within BWI76_RS14285 at 2987.520 kb on + strand, within BWI76_RS14285 at 2987.524 kb on + strand, within BWI76_RS14285 at 2987.534 kb on + strand, within BWI76_RS14285 at 2987.534 kb on + strand, within BWI76_RS14285
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,984,642 - BWI76_RS14270 0.31 -1.6 2,984,673 + BWI76_RS14270 0.34 -2.0 2,984,673 + BWI76_RS14270 0.34 +2.1 2,984,775 + BWI76_RS14270 0.47 -1.0 2,984,835 - +2.1 2,984,938 + BWI76_RS14270 0.67 +1.4 2,985,009 + BWI76_RS14270 0.76 +0.2 2,985,033 + BWI76_RS14270 0.79 -1.3 2,985,267 + -0.5 2,985,269 + +1.3 2,985,269 + -1.5 2,985,270 - +0.5 2,985,277 - -0.5 2,985,386 + +1.1 2,985,423 - +2.2 2,985,449 + +0.5 2,985,449 + -0.9 2,985,449 + -1.0 2,985,449 + -1.6 2,985,449 + +1.1 2,985,450 + +3.0 2,985,457 - +1.1 2,985,457 - -0.2 2,985,465 - +0.1 2,985,495 + +0.7 2,985,495 + -0.0 2,985,495 + -0.7 2,985,495 + -0.4 2,985,495 + -2.6 2,985,495 + -1.2 2,985,501 + +2.2 2,985,503 + -2.2 2,985,514 + -0.2 2,985,522 - +0.5 2,985,661 + +0.1 2,985,707 - +0.7 2,985,748 - BWI76_RS14275 0.13 +1.1 2,985,754 - BWI76_RS14275 0.13 -0.5 2,985,868 + BWI76_RS14275 0.23 -0.5 2,985,882 - BWI76_RS14275 0.24 +1.1 2,986,088 + BWI76_RS14275 0.41 -1.0 2,986,212 + BWI76_RS14275 0.52 -1.6 2,986,276 + BWI76_RS14275 0.57 -0.4 2,986,281 + BWI76_RS14275 0.57 +1.3 2,986,299 - BWI76_RS14275 0.59 -1.6 2,986,310 + BWI76_RS14275 0.60 -0.7 2,986,351 + BWI76_RS14275 0.63 -3.9 2,986,439 - BWI76_RS14275 0.71 -2.5 2,986,457 - BWI76_RS14275 0.72 -2.0 2,986,542 - BWI76_RS14275 0.79 -1.2 2,986,613 - BWI76_RS14275 0.85 -0.5 2,986,614 + BWI76_RS14275 0.85 +0.1 2,986,839 - -1.6 2,986,875 + -0.1 2,986,942 + BWI76_RS14280 0.30 -0.1 2,986,993 + BWI76_RS14280 0.50 +0.8 2,987,043 - BWI76_RS14280 0.69 +0.3 2,987,173 + +1.1 2,987,185 - +1.4 2,987,194 + -0.9 2,987,194 + -0.3 2,987,194 + -1.1 2,987,194 + -0.6 2,987,194 + -2.0 2,987,194 + -0.1 2,987,194 + +0.8 2,987,194 + +0.7 2,987,194 + -0.1 2,987,194 + -1.4 2,987,194 + -2.8 2,987,194 + -2.5 2,987,194 + +0.5 2,987,194 + -1.8 2,987,194 + -1.0 2,987,202 - +2.9 2,987,202 - -2.3 2,987,202 - +1.5 2,987,202 - -1.5 2,987,202 - +1.7 2,987,202 - -0.5 2,987,202 - -0.6 2,987,202 - -3.4 2,987,202 - -2.9 2,987,202 - +0.8 2,987,202 - -0.4 2,987,250 - -0.3 2,987,275 + -0.5 2,987,276 + -3.4 2,987,293 - -0.3 2,987,401 + -2.3 2,987,446 + +1.2 2,987,481 - BWI76_RS14285 0.17 +1.2 2,987,491 - BWI76_RS14285 0.20 +0.0 2,987,514 - BWI76_RS14285 0.28 -1.2 2,987,520 + BWI76_RS14285 0.30 -1.0 2,987,524 + BWI76_RS14285 0.32 -2.8 2,987,534 + BWI76_RS14285 0.35 -0.8 2,987,534 + BWI76_RS14285 0.35 -2.4
Or see this region's nucleotide sequence