Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14235 and BWI76_RS14240 are separated by 43 nucleotides BWI76_RS14240 and BWI76_RS14245 are separated by 35 nucleotides BWI76_RS14245 and BWI76_RS14250 are separated by 111 nucleotides
BWI76_RS14235: BWI76_RS14235 - alcohol dehydrogenase, at 2,978,303 to 2,979,327
_RS14235
BWI76_RS14240: BWI76_RS14240 - thiol reductase thioredoxin, at 2,979,371 to 2,979,694
_RS14240
BWI76_RS14245: BWI76_RS14245 - carboxymuconolactone decarboxylase family protein, at 2,979,730 to 2,980,251
_RS14245
BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781
_RS14250
Position (kb)
2979
2980
2981 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2978.744 kb on + strand, within BWI76_RS14235 at 2978.892 kb on + strand, within BWI76_RS14235 at 2978.900 kb on - strand, within BWI76_RS14235 at 2978.940 kb on + strand, within BWI76_RS14235 at 2979.367 kb on + strand at 2979.468 kb on - strand, within BWI76_RS14240 at 2979.563 kb on - strand, within BWI76_RS14240 at 2979.626 kb on + strand, within BWI76_RS14240 at 2979.634 kb on - strand, within BWI76_RS14240 at 2979.743 kb on + strand at 2979.796 kb on + strand, within BWI76_RS14245 at 2979.926 kb on + strand, within BWI76_RS14245 at 2979.943 kb on - strand, within BWI76_RS14245 at 2979.990 kb on - strand, within BWI76_RS14245 at 2980.261 kb on + strand at 2980.330 kb on - strand at 2980.581 kb on + strand at 2980.589 kb on - strand, within BWI76_RS14250 at 2980.703 kb on + strand, within BWI76_RS14250 at 2980.711 kb on - strand, within BWI76_RS14250 at 2980.778 kb on + strand, within BWI76_RS14250 at 2980.851 kb on - strand, within BWI76_RS14250 at 2980.870 kb on - strand, within BWI76_RS14250 at 2980.872 kb on - strand, within BWI76_RS14250 at 2980.875 kb on + strand, within BWI76_RS14250 at 2980.918 kb on + strand, within BWI76_RS14250 at 2980.918 kb on + strand, within BWI76_RS14250 at 2980.926 kb on - strand, within BWI76_RS14250 at 2980.935 kb on - strand, within BWI76_RS14250 at 2981.037 kb on - strand, within BWI76_RS14250 at 2981.158 kb on - strand, within BWI76_RS14250
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,978,744 + BWI76_RS14235 0.43 +0.6 2,978,892 + BWI76_RS14235 0.57 +1.1 2,978,900 - BWI76_RS14235 0.58 -2.8 2,978,940 + BWI76_RS14235 0.62 -1.8 2,979,367 + +2.8 2,979,468 - BWI76_RS14240 0.30 +0.6 2,979,563 - BWI76_RS14240 0.59 -1.3 2,979,626 + BWI76_RS14240 0.79 +0.3 2,979,634 - BWI76_RS14240 0.81 +0.3 2,979,743 + +0.8 2,979,796 + BWI76_RS14245 0.13 -3.0 2,979,926 + BWI76_RS14245 0.38 -0.3 2,979,943 - BWI76_RS14245 0.41 -0.5 2,979,990 - BWI76_RS14245 0.50 -1.3 2,980,261 + -2.0 2,980,330 - -1.0 2,980,581 + +2.1 2,980,589 - BWI76_RS14250 0.16 +0.8 2,980,703 + BWI76_RS14250 0.24 -0.2 2,980,711 - BWI76_RS14250 0.25 -2.7 2,980,778 + BWI76_RS14250 0.29 -0.5 2,980,851 - BWI76_RS14250 0.34 -0.9 2,980,870 - BWI76_RS14250 0.36 +0.8 2,980,872 - BWI76_RS14250 0.36 -0.2 2,980,875 + BWI76_RS14250 0.36 +0.1 2,980,918 + BWI76_RS14250 0.39 -0.1 2,980,918 + BWI76_RS14250 0.39 +0.6 2,980,926 - BWI76_RS14250 0.40 -0.4 2,980,935 - BWI76_RS14250 0.40 +1.0 2,981,037 - BWI76_RS14250 0.47 +0.7 2,981,158 - BWI76_RS14250 0.56 -3.1
Or see this region's nucleotide sequence