Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13765 and BWI76_RS13770 are separated by 5 nucleotides BWI76_RS13770 and BWI76_RS13775 are separated by 9 nucleotides BWI76_RS13775 and BWI76_RS13780 overlap by 4 nucleotides
BWI76_RS13765: BWI76_RS13765 - MFS transporter AraJ, at 2,869,840 to 2,870,991
_RS13765
BWI76_RS13770: BWI76_RS13770 - alkylhydroperoxidase domain protein, at 2,870,997 to 2,872,088
_RS13770
BWI76_RS13775: BWI76_RS13775 - putative FMN-dependent luciferase-like monooxygenase, at 2,872,098 to 2,873,087
_RS13775
BWI76_RS13780: BWI76_RS13780 - ABC transporter ATP-binding protein, at 2,873,084 to 2,874,688
_RS13780
Position (kb)
2870
2871
2872
2873 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2870.009 kb on + strand, within BWI76_RS13765 at 2870.014 kb on + strand, within BWI76_RS13765 at 2870.101 kb on + strand, within BWI76_RS13765 at 2870.288 kb on - strand, within BWI76_RS13765 at 2870.288 kb on - strand, within BWI76_RS13765 at 2870.342 kb on - strand, within BWI76_RS13765 at 2870.347 kb on + strand, within BWI76_RS13765 at 2870.352 kb on + strand, within BWI76_RS13765 at 2870.355 kb on - strand, within BWI76_RS13765 at 2870.355 kb on - strand, within BWI76_RS13765 at 2870.472 kb on - strand, within BWI76_RS13765 at 2870.501 kb on + strand, within BWI76_RS13765 at 2870.501 kb on - strand, within BWI76_RS13765 at 2870.700 kb on + strand, within BWI76_RS13765 at 2870.700 kb on + strand, within BWI76_RS13765 at 2870.708 kb on - strand, within BWI76_RS13765 at 2870.708 kb on - strand, within BWI76_RS13765 at 2870.708 kb on - strand, within BWI76_RS13765 at 2870.743 kb on + strand, within BWI76_RS13765 at 2870.765 kb on - strand, within BWI76_RS13765 at 2870.806 kb on + strand, within BWI76_RS13765 at 2870.906 kb on + strand at 2871.274 kb on + strand, within BWI76_RS13770 at 2871.753 kb on - strand, within BWI76_RS13770 at 2871.838 kb on + strand, within BWI76_RS13770 at 2871.993 kb on + strand at 2872.152 kb on + strand at 2872.166 kb on + strand at 2872.272 kb on - strand, within BWI76_RS13775 at 2872.532 kb on + strand, within BWI76_RS13775 at 2872.598 kb on - strand, within BWI76_RS13775
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,870,009 + BWI76_RS13765 0.15 +2.5 2,870,014 + BWI76_RS13765 0.15 -2.3 2,870,101 + BWI76_RS13765 0.23 -0.7 2,870,288 - BWI76_RS13765 0.39 -1.1 2,870,288 - BWI76_RS13765 0.39 +1.4 2,870,342 - BWI76_RS13765 0.44 +1.3 2,870,347 + BWI76_RS13765 0.44 +0.1 2,870,352 + BWI76_RS13765 0.44 -1.7 2,870,355 - BWI76_RS13765 0.45 -0.5 2,870,355 - BWI76_RS13765 0.45 -3.4 2,870,472 - BWI76_RS13765 0.55 -2.8 2,870,501 + BWI76_RS13765 0.57 -1.9 2,870,501 - BWI76_RS13765 0.57 -1.3 2,870,700 + BWI76_RS13765 0.75 -0.7 2,870,700 + BWI76_RS13765 0.75 +0.8 2,870,708 - BWI76_RS13765 0.75 -0.2 2,870,708 - BWI76_RS13765 0.75 +1.2 2,870,708 - BWI76_RS13765 0.75 +2.2 2,870,743 + BWI76_RS13765 0.78 -0.1 2,870,765 - BWI76_RS13765 0.80 +0.8 2,870,806 + BWI76_RS13765 0.84 -1.6 2,870,906 + -0.6 2,871,274 + BWI76_RS13770 0.25 -0.9 2,871,753 - BWI76_RS13770 0.69 -0.1 2,871,838 + BWI76_RS13770 0.77 -2.8 2,871,993 + -2.4 2,872,152 + -1.7 2,872,166 + +1.2 2,872,272 - BWI76_RS13775 0.18 -2.0 2,872,532 + BWI76_RS13775 0.44 -1.4 2,872,598 - BWI76_RS13775 0.51 -1.0
Or see this region's nucleotide sequence