Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10850

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10840 and BWI76_RS10845 are separated by 122 nucleotidesBWI76_RS10845 and BWI76_RS10850 overlap by 4 nucleotidesBWI76_RS10850 and BWI76_RS10855 are separated by 78 nucleotides BWI76_RS10840: BWI76_RS10840 - membrane protein, at 2,290,506 to 2,291,294 _RS10840 BWI76_RS10845: BWI76_RS10845 - DNA-binding response regulator, at 2,291,417 to 2,292,112 _RS10845 BWI76_RS10850: BWI76_RS10850 - two-component sensor histidine kinase, at 2,292,109 to 2,293,305 _RS10850 BWI76_RS10855: BWI76_RS10855 - RND family efflux transporter MFP subunit, at 2,293,384 to 2,294,532 _RS10855 Position (kb) 2292 2293 2294Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2291.271 kb on - strandat 2291.536 kb on + strand, within BWI76_RS10845at 2291.544 kb on - strand, within BWI76_RS10845at 2291.617 kb on - strand, within BWI76_RS10845at 2291.719 kb on + strand, within BWI76_RS10845at 2291.720 kb on + strand, within BWI76_RS10845at 2292.001 kb on - strand, within BWI76_RS10845at 2292.097 kb on - strandat 2292.325 kb on + strand, within BWI76_RS10850at 2292.438 kb on + strand, within BWI76_RS10850at 2292.729 kb on + strand, within BWI76_RS10850at 2292.958 kb on + strand, within BWI76_RS10850at 2293.394 kb on + strandat 2293.427 kb on - strandat 2293.486 kb on + strandat 2293.656 kb on + strand, within BWI76_RS10855at 2294.037 kb on - strand, within BWI76_RS10855

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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2,291,271 - -4.7
2,291,536 + BWI76_RS10845 0.17 +0.4
2,291,544 - BWI76_RS10845 0.18 +0.8
2,291,617 - BWI76_RS10845 0.29 +0.8
2,291,719 + BWI76_RS10845 0.43 +0.8
2,291,720 + BWI76_RS10845 0.44 -0.3
2,292,001 - BWI76_RS10845 0.84 +0.3
2,292,097 - -1.3
2,292,325 + BWI76_RS10850 0.18 -0.6
2,292,438 + BWI76_RS10850 0.27 -1.1
2,292,729 + BWI76_RS10850 0.52 -0.6
2,292,958 + BWI76_RS10850 0.71 -1.5
2,293,394 + -3.1
2,293,427 - -3.0
2,293,486 + +0.7
2,293,656 + BWI76_RS10855 0.24 -0.7
2,294,037 - BWI76_RS10855 0.57 -0.5

Or see this region's nucleotide sequence