Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10610 and BWI76_RS10615 are separated by 182 nucleotides BWI76_RS10615 and BWI76_RS10620 are separated by 130 nucleotides
BWI76_RS10610: BWI76_RS10610 - helix-turn-helix transcriptional regulator, at 2,241,450 to 2,241,998
_RS10610
BWI76_RS10615: BWI76_RS10615 - chorismate mutase, at 2,242,181 to 2,242,477
_RS10615
BWI76_RS10620: BWI76_RS10620 - hypothetical protein, at 2,242,608 to 2,243,672
_RS10620
Position (kb)
2242
2243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2241.296 kb on + strand at 2241.314 kb on + strand at 2241.323 kb on + strand at 2241.331 kb on - strand at 2241.331 kb on - strand at 2241.353 kb on + strand at 2241.373 kb on - strand at 2241.379 kb on + strand at 2241.402 kb on + strand at 2241.435 kb on + strand at 2241.435 kb on + strand at 2241.525 kb on + strand at 2241.525 kb on + strand, within BWI76_RS10610 at 2241.525 kb on + strand, within BWI76_RS10610 at 2241.531 kb on + strand, within BWI76_RS10610 at 2241.531 kb on + strand, within BWI76_RS10610 at 2241.531 kb on + strand, within BWI76_RS10610 at 2241.578 kb on + strand, within BWI76_RS10610 at 2241.586 kb on - strand, within BWI76_RS10610 at 2241.611 kb on + strand, within BWI76_RS10610 at 2241.616 kb on + strand, within BWI76_RS10610 at 2241.725 kb on + strand, within BWI76_RS10610 at 2241.892 kb on + strand, within BWI76_RS10610 at 2242.116 kb on + strand at 2242.214 kb on - strand, within BWI76_RS10615 at 2242.302 kb on + strand, within BWI76_RS10615 at 2242.308 kb on - strand, within BWI76_RS10615 at 2242.408 kb on + strand, within BWI76_RS10615 at 2242.408 kb on - strand, within BWI76_RS10615 at 2242.422 kb on + strand at 2242.422 kb on + strand, within BWI76_RS10615 at 2242.430 kb on - strand, within BWI76_RS10615 at 2242.452 kb on + strand at 2242.545 kb on + strand at 2242.567 kb on - strand at 2242.662 kb on + strand at 2242.707 kb on + strand at 2242.760 kb on + strand, within BWI76_RS10620 at 2242.796 kb on + strand, within BWI76_RS10620 at 2242.888 kb on + strand, within BWI76_RS10620 at 2242.922 kb on - strand, within BWI76_RS10620 at 2242.938 kb on + strand, within BWI76_RS10620 at 2242.943 kb on + strand, within BWI76_RS10620 at 2242.949 kb on - strand, within BWI76_RS10620 at 2242.951 kb on - strand, within BWI76_RS10620 at 2243.086 kb on + strand at 2243.094 kb on - strand, within BWI76_RS10620 at 2243.133 kb on - strand, within BWI76_RS10620 at 2243.168 kb on - strand, within BWI76_RS10620 at 2243.194 kb on + strand, within BWI76_RS10620 at 2243.217 kb on - strand, within BWI76_RS10620 at 2243.449 kb on + strand, within BWI76_RS10620 at 2243.450 kb on + strand, within BWI76_RS10620 at 2243.464 kb on + strand, within BWI76_RS10620 at 2243.472 kb on + strand, within BWI76_RS10620 at 2243.472 kb on - strand, within BWI76_RS10620
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,241,296 + -0.5 2,241,314 + -0.0 2,241,323 + -2.7 2,241,331 - +1.6 2,241,331 - -0.7 2,241,353 + +0.1 2,241,373 - -0.7 2,241,379 + +2.8 2,241,402 + +1.3 2,241,435 + -1.7 2,241,435 + -0.1 2,241,525 + +1.1 2,241,525 + BWI76_RS10610 0.14 -0.1 2,241,525 + BWI76_RS10610 0.14 +0.1 2,241,531 + BWI76_RS10610 0.15 -1.8 2,241,531 + BWI76_RS10610 0.15 +0.5 2,241,531 + BWI76_RS10610 0.15 +0.3 2,241,578 + BWI76_RS10610 0.23 +0.2 2,241,586 - BWI76_RS10610 0.25 +0.5 2,241,611 + BWI76_RS10610 0.29 +0.2 2,241,616 + BWI76_RS10610 0.30 +1.9 2,241,725 + BWI76_RS10610 0.50 -1.1 2,241,892 + BWI76_RS10610 0.81 +0.1 2,242,116 + +3.2 2,242,214 - BWI76_RS10615 0.11 -2.1 2,242,302 + BWI76_RS10615 0.41 -1.4 2,242,308 - BWI76_RS10615 0.43 -0.3 2,242,408 + BWI76_RS10615 0.76 -1.2 2,242,408 - BWI76_RS10615 0.76 -1.1 2,242,422 + +0.5 2,242,422 + BWI76_RS10615 0.81 -2.9 2,242,430 - BWI76_RS10615 0.84 -0.3 2,242,452 + -0.4 2,242,545 + -3.0 2,242,567 - -1.6 2,242,662 + -0.3 2,242,707 + -0.7 2,242,760 + BWI76_RS10620 0.14 -2.4 2,242,796 + BWI76_RS10620 0.18 +0.1 2,242,888 + BWI76_RS10620 0.26 -1.6 2,242,922 - BWI76_RS10620 0.29 -1.4 2,242,938 + BWI76_RS10620 0.31 +1.0 2,242,943 + BWI76_RS10620 0.31 -2.1 2,242,949 - BWI76_RS10620 0.32 -0.1 2,242,951 - BWI76_RS10620 0.32 +0.5 2,243,086 + +2.5 2,243,094 - BWI76_RS10620 0.46 +0.3 2,243,133 - BWI76_RS10620 0.49 +2.1 2,243,168 - BWI76_RS10620 0.53 +0.6 2,243,194 + BWI76_RS10620 0.55 +1.7 2,243,217 - BWI76_RS10620 0.57 -2.6 2,243,449 + BWI76_RS10620 0.79 -1.6 2,243,450 + BWI76_RS10620 0.79 -0.9 2,243,464 + BWI76_RS10620 0.80 -2.5 2,243,472 + BWI76_RS10620 0.81 -0.1 2,243,472 - BWI76_RS10620 0.81 +0.4
Or see this region's nucleotide sequence