Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09545

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09540 and BWI76_RS09545 are separated by 96 nucleotidesBWI76_RS09545 and BWI76_RS09550 are separated by 10 nucleotides BWI76_RS09540: BWI76_RS09540 - hypothetical protein, at 1,994,714 to 1,995,727 _RS09540 BWI76_RS09545: BWI76_RS09545 - NAD-dependent epimerase/dehydratase, at 1,995,824 to 1,997,257 _RS09545 BWI76_RS09550: BWI76_RS09550 - low-specificity L-threonine aldolase, at 1,997,268 to 1,998,269 _RS09550 Position (kb) 1995 1996 1997 1998Strain fitness (log2 ratio) -2 -1 0 1at 1994.934 kb on + strand, within BWI76_RS09540at 1995.367 kb on + strand, within BWI76_RS09540at 1995.592 kb on - strand, within BWI76_RS09540at 1995.999 kb on - strand, within BWI76_RS09545at 1995.999 kb on - strand, within BWI76_RS09545at 1996.322 kb on - strand, within BWI76_RS09545at 1996.669 kb on - strand, within BWI76_RS09545at 1996.874 kb on + strand, within BWI76_RS09545at 1997.060 kb on - strand, within BWI76_RS09545at 1997.075 kb on + strand, within BWI76_RS09545at 1997.092 kb on + strand, within BWI76_RS09545at 1997.274 kb on - strandat 1997.370 kb on - strand, within BWI76_RS09550at 1997.375 kb on - strand, within BWI76_RS09550at 1997.556 kb on - strand, within BWI76_RS09550at 1997.744 kb on - strand, within BWI76_RS09550at 1997.750 kb on - strand, within BWI76_RS09550at 1998.198 kb on - strandat 1998.235 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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1,994,934 + BWI76_RS09540 0.22 -2.1
1,995,367 + BWI76_RS09540 0.64 -1.6
1,995,592 - BWI76_RS09540 0.87 -1.1
1,995,999 - BWI76_RS09545 0.12 -0.7
1,995,999 - BWI76_RS09545 0.12 +0.4
1,996,322 - BWI76_RS09545 0.35 -0.5
1,996,669 - BWI76_RS09545 0.59 +0.7
1,996,874 + BWI76_RS09545 0.73 +0.5
1,997,060 - BWI76_RS09545 0.86 +0.3
1,997,075 + BWI76_RS09545 0.87 -2.4
1,997,092 + BWI76_RS09545 0.88 +0.6
1,997,274 - +0.9
1,997,370 - BWI76_RS09550 0.10 +1.7
1,997,375 - BWI76_RS09550 0.11 +0.7
1,997,556 - BWI76_RS09550 0.29 +0.6
1,997,744 - BWI76_RS09550 0.48 -2.2
1,997,750 - BWI76_RS09550 0.48 +0.7
1,998,198 - -1.0
1,998,235 + +0.2

Or see this region's nucleotide sequence