Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09470 and BWI76_RS09475 overlap by 4 nucleotides BWI76_RS09475 and BWI76_RS09480 are separated by 57 nucleotides BWI76_RS09480 and BWI76_RS09485 are separated by 42 nucleotides
BWI76_RS09470: BWI76_RS09470 - putrescine ABC transporter permease, at 1,982,935 to 1,983,888
_RS09470
BWI76_RS09475: BWI76_RS09475 - putrescine ABC transporter permease PotI, at 1,983,885 to 1,984,730
_RS09475
BWI76_RS09480: BWI76_RS09480 - hypothetical protein, at 1,984,788 to 1,985,276
_RS09480
BWI76_RS09485: BWI76_RS09485 - 23S rRNA (uracil(747)-C(5))-methyltransferase, at 1,985,319 to 1,986,449
_RS09485
Position (kb) 1984
1985
1986 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1983.802 kb on - strand at 1984.331 kb on + strand, within BWI76_RS09475 at 1984.339 kb on - strand, within BWI76_RS09475 at 1984.378 kb on + strand, within BWI76_RS09475 at 1984.495 kb on + strand, within BWI76_RS09475 at 1984.503 kb on - strand, within BWI76_RS09475 at 1984.710 kb on - strand at 1984.777 kb on - strand at 1984.847 kb on - strand, within BWI76_RS09480 at 1984.859 kb on + strand, within BWI76_RS09480 at 1984.973 kb on - strand, within BWI76_RS09480 at 1984.973 kb on - strand, within BWI76_RS09480 at 1985.132 kb on + strand, within BWI76_RS09480 at 1985.196 kb on - strand, within BWI76_RS09480 at 1985.279 kb on + strand at 1985.455 kb on - strand, within BWI76_RS09485 at 1985.681 kb on + strand, within BWI76_RS09485 at 1985.690 kb on - strand, within BWI76_RS09485 at 1985.776 kb on - strand, within BWI76_RS09485 at 1986.046 kb on - strand, within BWI76_RS09485 at 1986.063 kb on - strand, within BWI76_RS09485
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 1,983,802 - +1.2 1,984,331 + BWI76_RS09475 0.53 -1.0 1,984,339 - BWI76_RS09475 0.54 -1.4 1,984,378 + BWI76_RS09475 0.58 +1.3 1,984,495 + BWI76_RS09475 0.72 -0.2 1,984,503 - BWI76_RS09475 0.73 -3.3 1,984,710 - +0.6 1,984,777 - +0.6 1,984,847 - BWI76_RS09480 0.12 -1.6 1,984,859 + BWI76_RS09480 0.15 -1.3 1,984,973 - BWI76_RS09480 0.38 -0.4 1,984,973 - BWI76_RS09480 0.38 -1.0 1,985,132 + BWI76_RS09480 0.70 -3.6 1,985,196 - BWI76_RS09480 0.83 +0.0 1,985,279 + +0.9 1,985,455 - BWI76_RS09485 0.12 +0.8 1,985,681 + BWI76_RS09485 0.32 +0.3 1,985,690 - BWI76_RS09485 0.33 -2.1 1,985,776 - BWI76_RS09485 0.40 +0.4 1,986,046 - BWI76_RS09485 0.64 -1.4 1,986,063 - BWI76_RS09485 0.66 +0.6
Or see this region's nucleotide sequence