Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06250 and BWI76_RS06255 overlap by 4 nucleotides BWI76_RS06255 and BWI76_RS06260 are separated by 291 nucleotides
BWI76_RS06250: BWI76_RS06250 - alpha-glycosidase, at 1,313,128 to 1,314,945
_RS06250
BWI76_RS06255: BWI76_RS06255 - LysR family transcriptional regulator, at 1,314,942 to 1,315,844
_RS06255
BWI76_RS06260: BWI76_RS06260 - hydrolase, at 1,316,136 to 1,316,777
_RS06260
Position (kb)
1314
1315
1316 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1314.354 kb on + strand, within BWI76_RS06250 at 1314.639 kb on - strand, within BWI76_RS06250 at 1314.924 kb on - strand at 1314.946 kb on + strand at 1314.954 kb on - strand at 1315.004 kb on - strand at 1315.134 kb on + strand, within BWI76_RS06255 at 1315.461 kb on - strand, within BWI76_RS06255 at 1315.462 kb on - strand, within BWI76_RS06255 at 1315.477 kb on - strand, within BWI76_RS06255 at 1315.569 kb on + strand, within BWI76_RS06255 at 1315.708 kb on - strand, within BWI76_RS06255 at 1315.710 kb on - strand, within BWI76_RS06255 at 1315.735 kb on + strand, within BWI76_RS06255 at 1315.748 kb on - strand, within BWI76_RS06255 at 1315.753 kb on - strand, within BWI76_RS06255 at 1315.824 kb on + strand at 1315.825 kb on + strand at 1315.825 kb on + strand at 1315.826 kb on + strand at 1315.826 kb on + strand at 1315.826 kb on + strand at 1315.826 kb on + strand at 1315.826 kb on + strand at 1315.826 kb on + strand at 1315.831 kb on + strand at 1315.833 kb on + strand at 1315.833 kb on - strand at 1315.833 kb on - strand at 1315.834 kb on - strand at 1315.834 kb on - strand at 1315.834 kb on - strand at 1315.834 kb on - strand at 1315.834 kb on - strand at 1315.834 kb on - strand at 1315.841 kb on - strand at 1315.841 kb on - strand at 1315.841 kb on - strand at 1315.841 kb on - strand at 1315.841 kb on - strand at 1315.847 kb on + strand at 1315.870 kb on + strand at 1315.871 kb on - strand at 1315.878 kb on - strand at 1315.894 kb on + strand at 1315.894 kb on + strand at 1315.894 kb on + strand at 1315.894 kb on + strand at 1315.894 kb on + strand at 1315.894 kb on + strand at 1315.894 kb on + strand at 1315.894 kb on + strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1315.902 kb on - strand at 1316.049 kb on + strand at 1316.049 kb on + strand at 1316.049 kb on + strand at 1316.051 kb on + strand at 1316.061 kb on - strand at 1316.062 kb on - strand at 1316.073 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.074 kb on + strand at 1316.081 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.082 kb on - strand at 1316.084 kb on + strand at 1316.084 kb on + strand at 1316.084 kb on + strand at 1316.084 kb on + strand at 1316.084 kb on + strand at 1316.084 kb on + strand at 1316.084 kb on + strand at 1316.087 kb on + strand at 1316.092 kb on - strand at 1316.092 kb on - strand at 1316.092 kb on - strand at 1316.092 kb on - strand at 1316.092 kb on - strand at 1316.092 kb on - strand at 1316.104 kb on - strand at 1316.104 kb on - strand at 1316.135 kb on - strand at 1316.135 kb on - strand at 1316.151 kb on + strand at 1316.161 kb on - strand at 1316.206 kb on + strand, within BWI76_RS06260 at 1316.255 kb on + strand, within BWI76_RS06260 at 1316.256 kb on + strand, within BWI76_RS06260 at 1316.256 kb on + strand, within BWI76_RS06260 at 1316.256 kb on + strand, within BWI76_RS06260 at 1316.256 kb on + strand, within BWI76_RS06260 at 1316.264 kb on - strand, within BWI76_RS06260 at 1316.284 kb on - strand, within BWI76_RS06260 at 1316.295 kb on + strand, within BWI76_RS06260 at 1316.303 kb on - strand, within BWI76_RS06260 at 1316.303 kb on - strand, within BWI76_RS06260 at 1316.303 kb on - strand at 1316.320 kb on + strand, within BWI76_RS06260 at 1316.328 kb on + strand, within BWI76_RS06260 at 1316.328 kb on + strand, within BWI76_RS06260 at 1316.328 kb on + strand, within BWI76_RS06260 at 1316.328 kb on + strand, within BWI76_RS06260 at 1316.329 kb on + strand, within BWI76_RS06260 at 1316.329 kb on + strand, within BWI76_RS06260 at 1316.329 kb on + strand, within BWI76_RS06260 at 1316.336 kb on - strand, within BWI76_RS06260 at 1316.336 kb on - strand at 1316.336 kb on - strand, within BWI76_RS06260 at 1316.336 kb on - strand, within BWI76_RS06260 at 1316.336 kb on - strand, within BWI76_RS06260 at 1316.336 kb on - strand, within BWI76_RS06260 at 1316.336 kb on - strand, within BWI76_RS06260 at 1316.337 kb on - strand, within BWI76_RS06260 at 1316.338 kb on + strand, within BWI76_RS06260 at 1316.338 kb on + strand, within BWI76_RS06260 at 1316.343 kb on + strand, within BWI76_RS06260 at 1316.346 kb on - strand, within BWI76_RS06260 at 1316.351 kb on - strand, within BWI76_RS06260 at 1316.354 kb on + strand, within BWI76_RS06260 at 1316.360 kb on - strand, within BWI76_RS06260 at 1316.363 kb on - strand, within BWI76_RS06260 at 1316.372 kb on - strand, within BWI76_RS06260 at 1316.374 kb on + strand, within BWI76_RS06260 at 1316.374 kb on + strand, within BWI76_RS06260 at 1316.375 kb on + strand, within BWI76_RS06260 at 1316.375 kb on - strand, within BWI76_RS06260 at 1316.382 kb on - strand, within BWI76_RS06260 at 1316.393 kb on - strand, within BWI76_RS06260 at 1316.395 kb on + strand, within BWI76_RS06260 at 1316.396 kb on + strand, within BWI76_RS06260 at 1316.398 kb on + strand, within BWI76_RS06260 at 1316.398 kb on + strand, within BWI76_RS06260 at 1316.398 kb on + strand, within BWI76_RS06260 at 1316.398 kb on + strand, within BWI76_RS06260 at 1316.398 kb on + strand, within BWI76_RS06260 at 1316.398 kb on + strand, within BWI76_RS06260 at 1316.404 kb on - strand, within BWI76_RS06260 at 1316.404 kb on - strand, within BWI76_RS06260 at 1316.404 kb on - strand, within BWI76_RS06260 at 1316.406 kb on - strand, within BWI76_RS06260 at 1316.406 kb on - strand, within BWI76_RS06260 at 1316.406 kb on - strand, within BWI76_RS06260 at 1316.406 kb on - strand, within BWI76_RS06260 at 1316.406 kb on - strand, within BWI76_RS06260 at 1316.406 kb on - strand, within BWI76_RS06260 at 1316.468 kb on + strand, within BWI76_RS06260 at 1316.476 kb on - strand, within BWI76_RS06260 at 1316.481 kb on + strand, within BWI76_RS06260 at 1316.489 kb on - strand, within BWI76_RS06260 at 1316.489 kb on - strand, within BWI76_RS06260 at 1316.525 kb on + strand, within BWI76_RS06260 at 1316.553 kb on - strand, within BWI76_RS06260 at 1316.737 kb on - strand at 1316.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 1,314,354 + BWI76_RS06250 0.67 -0.1 1,314,639 - BWI76_RS06250 0.83 -3.4 1,314,924 - -0.5 1,314,946 + -1.7 1,314,954 - +0.6 1,315,004 - +0.3 1,315,134 + BWI76_RS06255 0.21 -1.6 1,315,461 - BWI76_RS06255 0.57 -0.2 1,315,462 - BWI76_RS06255 0.58 +0.8 1,315,477 - BWI76_RS06255 0.59 +1.2 1,315,569 + BWI76_RS06255 0.69 -0.4 1,315,708 - BWI76_RS06255 0.85 -1.3 1,315,710 - BWI76_RS06255 0.85 +4.0 1,315,735 + BWI76_RS06255 0.88 +0.6 1,315,748 - BWI76_RS06255 0.89 +1.7 1,315,753 - BWI76_RS06255 0.90 +1.5 1,315,824 + +1.5 1,315,825 + -2.6 1,315,825 + -1.2 1,315,826 + +1.0 1,315,826 + -1.8 1,315,826 + -1.5 1,315,826 + -1.7 1,315,826 + -0.3 1,315,826 + +1.4 1,315,831 + -0.2 1,315,833 + -1.0 1,315,833 - +0.1 1,315,833 - -0.4 1,315,834 - +1.1 1,315,834 - -1.6 1,315,834 - -0.6 1,315,834 - +0.5 1,315,834 - +1.1 1,315,834 - -0.5 1,315,841 - +2.2 1,315,841 - -0.1 1,315,841 - +0.2 1,315,841 - -0.9 1,315,841 - +0.3 1,315,847 + -1.3 1,315,870 + -0.3 1,315,871 - -2.1 1,315,878 - +1.0 1,315,894 + +0.2 1,315,894 + +2.0 1,315,894 + -0.2 1,315,894 + +0.6 1,315,894 + -2.4 1,315,894 + -2.8 1,315,894 + -0.8 1,315,894 + +2.4 1,315,902 - -3.3 1,315,902 - -0.3 1,315,902 - -1.8 1,315,902 - -0.0 1,315,902 - -0.7 1,315,902 - +1.1 1,315,902 - +0.7 1,315,902 - +1.0 1,315,902 - -1.5 1,315,902 - -3.1 1,315,902 - +0.6 1,315,902 - +0.9 1,315,902 - -0.0 1,315,902 - +0.5 1,316,049 + -0.3 1,316,049 + +0.9 1,316,049 + -0.4 1,316,051 + -0.8 1,316,061 - +0.4 1,316,062 - +0.1 1,316,073 + -1.4 1,316,074 + -0.2 1,316,074 + +0.5 1,316,074 + +2.1 1,316,074 + -0.3 1,316,074 + +0.1 1,316,074 + +1.4 1,316,074 + -0.5 1,316,074 + -1.7 1,316,074 + -1.0 1,316,074 + +1.2 1,316,074 + -0.4 1,316,074 + +1.0 1,316,074 + +1.1 1,316,074 + -0.3 1,316,074 + +0.5 1,316,074 + -0.4 1,316,074 + +0.5 1,316,074 + +0.3 1,316,074 + +0.9 1,316,074 + +0.2 1,316,074 + -1.1 1,316,074 + -0.5 1,316,081 - +1.4 1,316,082 - -0.3 1,316,082 - -0.9 1,316,082 - +1.0 1,316,082 - -1.0 1,316,082 - +0.8 1,316,082 - -2.5 1,316,082 - -1.8 1,316,082 - +0.8 1,316,082 - -0.1 1,316,082 - -0.7 1,316,082 - -1.9 1,316,082 - -0.5 1,316,082 - +4.2 1,316,082 - -0.6 1,316,082 - +0.1 1,316,082 - +0.6 1,316,082 - +1.8 1,316,082 - +0.5 1,316,082 - +3.5 1,316,082 - +0.4 1,316,084 + -0.1 1,316,084 + +0.7 1,316,084 + +1.3 1,316,084 + +0.1 1,316,084 + +0.5 1,316,084 + +0.2 1,316,084 + -0.4 1,316,087 + -2.9 1,316,092 - -0.7 1,316,092 - -2.6 1,316,092 - +0.6 1,316,092 - +0.3 1,316,092 - -0.4 1,316,092 - +2.1 1,316,104 - +0.2 1,316,104 - +1.5 1,316,135 - +0.5 1,316,135 - -0.3 1,316,151 + -1.3 1,316,161 - +0.4 1,316,206 + BWI76_RS06260 0.11 -0.2 1,316,255 + BWI76_RS06260 0.19 -0.5 1,316,256 + BWI76_RS06260 0.19 +1.3 1,316,256 + BWI76_RS06260 0.19 -3.7 1,316,256 + BWI76_RS06260 0.19 +0.0 1,316,256 + BWI76_RS06260 0.19 +0.7 1,316,264 - BWI76_RS06260 0.20 -0.9 1,316,284 - BWI76_RS06260 0.23 +0.5 1,316,295 + BWI76_RS06260 0.25 -0.7 1,316,303 - BWI76_RS06260 0.26 -0.2 1,316,303 - BWI76_RS06260 0.26 -1.0 1,316,303 - -0.0 1,316,320 + BWI76_RS06260 0.29 +0.6 1,316,328 + BWI76_RS06260 0.30 +0.5 1,316,328 + BWI76_RS06260 0.30 +0.9 1,316,328 + BWI76_RS06260 0.30 +0.8 1,316,328 + BWI76_RS06260 0.30 +1.8 1,316,329 + BWI76_RS06260 0.30 -0.2 1,316,329 + BWI76_RS06260 0.30 +1.5 1,316,329 + BWI76_RS06260 0.30 +1.1 1,316,336 - BWI76_RS06260 0.31 -0.3 1,316,336 - +2.0 1,316,336 - BWI76_RS06260 0.31 +0.4 1,316,336 - BWI76_RS06260 0.31 +0.6 1,316,336 - BWI76_RS06260 0.31 -0.8 1,316,336 - BWI76_RS06260 0.31 -2.0 1,316,336 - BWI76_RS06260 0.31 -1.6 1,316,337 - BWI76_RS06260 0.31 +0.8 1,316,338 + BWI76_RS06260 0.31 -0.2 1,316,338 + BWI76_RS06260 0.31 +4.4 1,316,343 + BWI76_RS06260 0.32 -0.8 1,316,346 - BWI76_RS06260 0.33 -0.1 1,316,351 - BWI76_RS06260 0.33 +1.5 1,316,354 + BWI76_RS06260 0.34 -1.3 1,316,360 - BWI76_RS06260 0.35 -1.4 1,316,363 - BWI76_RS06260 0.35 +0.2 1,316,372 - BWI76_RS06260 0.37 +1.2 1,316,374 + BWI76_RS06260 0.37 +0.0 1,316,374 + BWI76_RS06260 0.37 -2.7 1,316,375 + BWI76_RS06260 0.37 +0.7 1,316,375 - BWI76_RS06260 0.37 -0.9 1,316,382 - BWI76_RS06260 0.38 +0.5 1,316,393 - BWI76_RS06260 0.40 +1.8 1,316,395 + BWI76_RS06260 0.40 -0.5 1,316,396 + BWI76_RS06260 0.40 +0.0 1,316,398 + BWI76_RS06260 0.41 -0.5 1,316,398 + BWI76_RS06260 0.41 +0.5 1,316,398 + BWI76_RS06260 0.41 -0.6 1,316,398 + BWI76_RS06260 0.41 +0.9 1,316,398 + BWI76_RS06260 0.41 -1.0 1,316,398 + BWI76_RS06260 0.41 +0.5 1,316,404 - BWI76_RS06260 0.42 -0.5 1,316,404 - BWI76_RS06260 0.42 -0.2 1,316,404 - BWI76_RS06260 0.42 +2.0 1,316,406 - BWI76_RS06260 0.42 +0.2 1,316,406 - BWI76_RS06260 0.42 -4.4 1,316,406 - BWI76_RS06260 0.42 -1.0 1,316,406 - BWI76_RS06260 0.42 -1.8 1,316,406 - BWI76_RS06260 0.42 -3.0 1,316,406 - BWI76_RS06260 0.42 +0.6 1,316,468 + BWI76_RS06260 0.52 +0.4 1,316,476 - BWI76_RS06260 0.53 -0.6 1,316,481 + BWI76_RS06260 0.54 -0.6 1,316,489 - BWI76_RS06260 0.55 -3.0 1,316,489 - BWI76_RS06260 0.55 -0.8 1,316,525 + BWI76_RS06260 0.61 -4.3 1,316,553 - BWI76_RS06260 0.65 +1.0 1,316,737 - -0.3 1,316,833 - +1.0
Or see this region's nucleotide sequence