Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04610 and BWI76_RS04615 overlap by 25 nucleotides BWI76_RS04615 and BWI76_RS04620 are separated by 162 nucleotides
BWI76_RS04610: BWI76_RS04610 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 959,191 to 960,801
_RS04610
BWI76_RS04615: BWI76_RS04615 - thiamine transporter substrate binding subunit, at 960,777 to 961,760
_RS04615
BWI76_RS04620: BWI76_RS04620 - transcriptional regulator SgrR, at 961,923 to 963,578
_RS04620
Position (kb) 960
961
962 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 959.799 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 960.041 kb on - strand at 960.227 kb on + strand, within BWI76_RS04610 at 960.236 kb on - strand, within BWI76_RS04610 at 960.254 kb on - strand, within BWI76_RS04610 at 960.445 kb on + strand, within BWI76_RS04610 at 960.448 kb on - strand, within BWI76_RS04610 at 960.506 kb on - strand, within BWI76_RS04610 at 960.555 kb on + strand, within BWI76_RS04610 at 960.909 kb on - strand, within BWI76_RS04615 at 961.024 kb on + strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.321 kb on + strand, within BWI76_RS04615 at 961.329 kb on - strand, within BWI76_RS04615 at 961.495 kb on + strand, within BWI76_RS04615 at 961.674 kb on - strand at 962.083 kb on + strand at 962.166 kb on - strand, within BWI76_RS04620 at 962.225 kb on - strand, within BWI76_RS04620 at 962.234 kb on + strand, within BWI76_RS04620 at 962.615 kb on + strand, within BWI76_RS04620 at 962.759 kb on + strand, within BWI76_RS04620
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 959,799 + BWI76_RS04610 0.38 +1.4 959,944 + BWI76_RS04610 0.47 +0.9 959,944 + BWI76_RS04610 0.47 +1.5 960,041 - -0.6 960,227 + BWI76_RS04610 0.64 -0.3 960,236 - BWI76_RS04610 0.65 +1.7 960,254 - BWI76_RS04610 0.66 -0.2 960,445 + BWI76_RS04610 0.78 +1.6 960,448 - BWI76_RS04610 0.78 +1.6 960,506 - BWI76_RS04610 0.82 -0.3 960,555 + BWI76_RS04610 0.85 +1.2 960,909 - BWI76_RS04615 0.13 +2.9 961,024 + BWI76_RS04615 0.25 +1.0 961,132 - BWI76_RS04615 0.36 +1.2 961,132 - BWI76_RS04615 0.36 +0.8 961,321 + BWI76_RS04615 0.55 +0.7 961,329 - BWI76_RS04615 0.56 +0.5 961,495 + BWI76_RS04615 0.73 -2.9 961,674 - -0.6 962,083 + -0.9 962,166 - BWI76_RS04620 0.15 +0.1 962,225 - BWI76_RS04620 0.18 -0.1 962,234 + BWI76_RS04620 0.19 -1.8 962,615 + BWI76_RS04620 0.42 +0.6 962,759 + BWI76_RS04620 0.50 -0.2
Or see this region's nucleotide sequence