Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02635 and BWI76_RS02640 are separated by 9 nucleotides BWI76_RS02640 and BWI76_RS02645 overlap by 1 nucleotides BWI76_RS02645 and BWI76_RS02650 are separated by 61 nucleotides
BWI76_RS02635: BWI76_RS02635 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 538,266 to 538,916
_RS02635
BWI76_RS02640: BWI76_RS02640 - xylulose 5-phosphate 3-epimerase, at 538,926 to 539,780
_RS02640
BWI76_RS02645: BWI76_RS02645 - L-ribulose-5-phosphate 4-epimerase, at 539,780 to 540,466
_RS02645
BWI76_RS02650: BWI76_RS02650 - MFS transporter, at 540,528 to 541,721
_RS02650
Position (kb) 539
540
541 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 538.810 kb on - strand, within BWI76_RS02635 at 538.883 kb on + strand at 538.891 kb on - strand at 538.904 kb on + strand at 538.980 kb on - strand at 539.103 kb on - strand, within BWI76_RS02640 at 539.103 kb on - strand, within BWI76_RS02640 at 539.103 kb on - strand, within BWI76_RS02640 at 539.113 kb on - strand, within BWI76_RS02640 at 539.187 kb on - strand, within BWI76_RS02640 at 539.335 kb on + strand, within BWI76_RS02640 at 539.335 kb on + strand, within BWI76_RS02640 at 539.427 kb on + strand, within BWI76_RS02640 at 539.435 kb on - strand, within BWI76_RS02640 at 539.514 kb on - strand, within BWI76_RS02640 at 539.546 kb on - strand, within BWI76_RS02640 at 539.566 kb on + strand, within BWI76_RS02640 at 539.629 kb on + strand, within BWI76_RS02640 at 539.755 kb on + strand at 539.765 kb on + strand at 539.773 kb on - strand at 539.778 kb on - strand at 539.860 kb on + strand, within BWI76_RS02645 at 539.967 kb on + strand, within BWI76_RS02645 at 540.053 kb on + strand, within BWI76_RS02645 at 540.053 kb on + strand, within BWI76_RS02645 at 540.061 kb on - strand, within BWI76_RS02645 at 540.080 kb on + strand, within BWI76_RS02645 at 540.087 kb on - strand, within BWI76_RS02645 at 540.184 kb on + strand, within BWI76_RS02645 at 540.245 kb on - strand, within BWI76_RS02645 at 540.372 kb on + strand, within BWI76_RS02645 at 540.385 kb on + strand, within BWI76_RS02645 at 540.516 kb on - strand at 540.585 kb on + strand at 540.644 kb on + strand at 540.652 kb on - strand, within BWI76_RS02650 at 540.652 kb on - strand, within BWI76_RS02650 at 540.673 kb on - strand, within BWI76_RS02650 at 540.673 kb on - strand, within BWI76_RS02650 at 540.805 kb on + strand, within BWI76_RS02650 at 540.852 kb on - strand at 540.892 kb on - strand, within BWI76_RS02650 at 540.892 kb on - strand, within BWI76_RS02650 at 541.050 kb on + strand, within BWI76_RS02650 at 541.079 kb on + strand, within BWI76_RS02650 at 541.106 kb on - strand, within BWI76_RS02650 at 541.113 kb on - strand, within BWI76_RS02650 at 541.115 kb on + strand, within BWI76_RS02650 at 541.178 kb on + strand, within BWI76_RS02650 at 541.351 kb on - strand, within BWI76_RS02650 at 541.457 kb on + strand, within BWI76_RS02650 at 541.457 kb on + strand, within BWI76_RS02650 at 541.457 kb on + strand at 541.465 kb on - strand, within BWI76_RS02650 at 541.465 kb on - strand, within BWI76_RS02650 at 541.465 kb on - strand, within BWI76_RS02650
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 538,810 - BWI76_RS02635 0.84 +1.0 538,883 + -0.8 538,891 - +0.8 538,904 + -1.8 538,980 - +2.3 539,103 - BWI76_RS02640 0.21 +0.1 539,103 - BWI76_RS02640 0.21 +0.5 539,103 - BWI76_RS02640 0.21 -3.2 539,113 - BWI76_RS02640 0.22 +1.3 539,187 - BWI76_RS02640 0.31 -0.2 539,335 + BWI76_RS02640 0.48 +0.1 539,335 + BWI76_RS02640 0.48 -0.8 539,427 + BWI76_RS02640 0.59 -1.6 539,435 - BWI76_RS02640 0.60 +0.6 539,514 - BWI76_RS02640 0.69 -2.1 539,546 - BWI76_RS02640 0.73 -2.0 539,566 + BWI76_RS02640 0.75 +0.0 539,629 + BWI76_RS02640 0.82 +1.1 539,755 + -0.1 539,765 + -1.6 539,773 - -0.5 539,778 - +0.3 539,860 + BWI76_RS02645 0.12 -2.1 539,967 + BWI76_RS02645 0.27 -0.5 540,053 + BWI76_RS02645 0.40 +0.2 540,053 + BWI76_RS02645 0.40 -0.6 540,061 - BWI76_RS02645 0.41 -0.4 540,080 + BWI76_RS02645 0.44 -0.1 540,087 - BWI76_RS02645 0.45 +0.9 540,184 + BWI76_RS02645 0.59 +0.1 540,245 - BWI76_RS02645 0.68 -0.4 540,372 + BWI76_RS02645 0.86 -1.5 540,385 + BWI76_RS02645 0.88 -1.3 540,516 - -2.0 540,585 + -0.3 540,644 + +0.4 540,652 - BWI76_RS02650 0.10 -1.2 540,652 - BWI76_RS02650 0.10 +0.4 540,673 - BWI76_RS02650 0.12 -2.3 540,673 - BWI76_RS02650 0.12 +0.3 540,805 + BWI76_RS02650 0.23 +0.7 540,852 - -0.4 540,892 - BWI76_RS02650 0.30 +0.4 540,892 - BWI76_RS02650 0.30 -2.9 541,050 + BWI76_RS02650 0.44 +0.3 541,079 + BWI76_RS02650 0.46 -1.0 541,106 - BWI76_RS02650 0.48 +1.0 541,113 - BWI76_RS02650 0.49 +0.5 541,115 + BWI76_RS02650 0.49 -0.4 541,178 + BWI76_RS02650 0.54 -1.1 541,351 - BWI76_RS02650 0.69 -0.2 541,457 + BWI76_RS02650 0.78 -1.8 541,457 + BWI76_RS02650 0.78 -1.3 541,457 + +0.7 541,465 - BWI76_RS02650 0.78 -1.3 541,465 - BWI76_RS02650 0.78 -0.6 541,465 - BWI76_RS02650 0.78 -3.4
Or see this region's nucleotide sequence