Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24755 and BWI76_RS24760 are separated by 46 nucleotides BWI76_RS24760 and BWI76_RS24765 are separated by 2 nucleotides BWI76_RS24765 and BWI76_RS24770 overlap by 4 nucleotides BWI76_RS24770 and BWI76_RS24775 are separated by 323 nucleotides BWI76_RS24775 and BWI76_RS24780 are separated by 74 nucleotides
BWI76_RS24755: BWI76_RS24755 - hypothetical protein, at 5,163,143 to 5,163,511
_RS24755
BWI76_RS24760: BWI76_RS24760 - membrane protein, at 5,163,558 to 5,163,863
_RS24760
BWI76_RS24765: BWI76_RS24765 - membrane protein, at 5,163,866 to 5,164,264
_RS24765
BWI76_RS24770: BWI76_RS24770 - hypothetical protein, at 5,164,261 to 5,164,560
_RS24770
BWI76_RS24775: BWI76_RS24775 - hypothetical protein, at 5,164,884 to 5,165,276
_RS24775
BWI76_RS24780: BWI76_RS24780 - glutathione-dependent reductase, at 5,165,351 to 5,166,337
_RS24780
Position (kb) 5164
5165 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5163.419 kb on - strand, within BWI76_RS24755 at 5163.489 kb on + strand at 5163.523 kb on + strand at 5163.917 kb on + strand, within BWI76_RS24765 at 5163.920 kb on - strand, within BWI76_RS24765 at 5163.927 kb on + strand, within BWI76_RS24765 at 5164.366 kb on - strand, within BWI76_RS24770 at 5164.387 kb on + strand, within BWI76_RS24770 at 5164.514 kb on - strand, within BWI76_RS24770 at 5164.579 kb on + strand at 5164.582 kb on - strand at 5164.685 kb on + strand at 5164.785 kb on - strand at 5164.859 kb on + strand at 5164.940 kb on + strand, within BWI76_RS24775 at 5165.116 kb on + strand, within BWI76_RS24775 at 5165.124 kb on - strand, within BWI76_RS24775 at 5165.227 kb on - strand, within BWI76_RS24775 at 5165.279 kb on + strand at 5165.311 kb on + strand at 5165.493 kb on - strand, within BWI76_RS24780 at 5165.516 kb on + strand, within BWI76_RS24780 at 5165.538 kb on + strand, within BWI76_RS24780
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 5,163,419 - BWI76_RS24755 0.75 +0.9 5,163,489 + -0.2 5,163,523 + +1.4 5,163,917 + BWI76_RS24765 0.13 -0.5 5,163,920 - BWI76_RS24765 0.14 +0.7 5,163,927 + BWI76_RS24765 0.15 -0.6 5,164,366 - BWI76_RS24770 0.35 -0.6 5,164,387 + BWI76_RS24770 0.42 -0.8 5,164,514 - BWI76_RS24770 0.84 -3.1 5,164,579 + +0.3 5,164,582 - -1.7 5,164,685 + +0.6 5,164,785 - +0.0 5,164,859 + +0.1 5,164,940 + BWI76_RS24775 0.14 -1.6 5,165,116 + BWI76_RS24775 0.59 +0.2 5,165,124 - BWI76_RS24775 0.61 +0.2 5,165,227 - BWI76_RS24775 0.87 +0.0 5,165,279 + -0.7 5,165,311 + -1.5 5,165,493 - BWI76_RS24780 0.14 -0.5 5,165,516 + BWI76_RS24780 0.17 -0.6 5,165,538 + BWI76_RS24780 0.19 +0.4
Or see this region's nucleotide sequence