Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22725 and BWI76_RS22730 overlap by 19 nucleotides BWI76_RS22730 and BWI76_RS22735 are separated by 9 nucleotides BWI76_RS22735 and BWI76_RS22740 are separated by 14 nucleotides BWI76_RS22740 and BWI76_RS22745 overlap by 4 nucleotides
BWI76_RS22725: BWI76_RS22725 - type I-E CRISPR-associated protein Cas6/Cse3/CasE, at 4,735,717 to 4,736,367
_RS22725
BWI76_RS22730: BWI76_RS22730 - type I-E CRISPR-associated protein Cas5/CasD, at 4,736,349 to 4,737,095
_RS22730
BWI76_RS22735: BWI76_RS22735 - type I-E CRISPR-associated protein Cas7/Cse4/CasC, at 4,737,105 to 4,738,163
_RS22735
BWI76_RS22740: BWI76_RS22740 - type I-E CRISPR-associated protein Cse2/CasB, at 4,738,178 to 4,738,729
_RS22740
BWI76_RS22745: BWI76_RS22745 - type I-E CRISPR-associated protein Cse1/CasA, at 4,738,726 to 4,740,285
_RS22745
Position (kb) 4737
4738
4739 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 4736.183 kb on - strand, within BWI76_RS22725 at 4736.187 kb on + strand, within BWI76_RS22725 at 4736.207 kb on + strand, within BWI76_RS22725 at 4736.329 kb on - strand at 4736.362 kb on + strand at 4736.370 kb on - strand at 4736.462 kb on - strand, within BWI76_RS22730 at 4736.463 kb on - strand, within BWI76_RS22730 at 4736.574 kb on + strand, within BWI76_RS22730 at 4736.680 kb on - strand, within BWI76_RS22730 at 4736.752 kb on + strand, within BWI76_RS22730 at 4736.760 kb on - strand, within BWI76_RS22730 at 4736.784 kb on + strand, within BWI76_RS22730 at 4736.789 kb on + strand, within BWI76_RS22730 at 4736.813 kb on - strand, within BWI76_RS22730 at 4736.885 kb on + strand, within BWI76_RS22730 at 4736.909 kb on - strand, within BWI76_RS22730 at 4736.990 kb on - strand, within BWI76_RS22730 at 4737.010 kb on - strand, within BWI76_RS22730 at 4737.129 kb on + strand at 4737.166 kb on - strand at 4737.215 kb on + strand, within BWI76_RS22735 at 4737.215 kb on + strand, within BWI76_RS22735 at 4737.215 kb on + strand, within BWI76_RS22735 at 4737.478 kb on + strand, within BWI76_RS22735 at 4737.602 kb on + strand, within BWI76_RS22735 at 4737.610 kb on - strand at 4737.806 kb on - strand, within BWI76_RS22735 at 4737.956 kb on + strand, within BWI76_RS22735 at 4738.074 kb on - strand at 4738.243 kb on - strand, within BWI76_RS22740 at 4738.264 kb on + strand, within BWI76_RS22740 at 4738.359 kb on + strand, within BWI76_RS22740 at 4738.359 kb on + strand, within BWI76_RS22740 at 4738.367 kb on - strand, within BWI76_RS22740 at 4738.367 kb on - strand, within BWI76_RS22740 at 4738.431 kb on - strand, within BWI76_RS22740 at 4738.494 kb on + strand, within BWI76_RS22740 at 4738.549 kb on + strand, within BWI76_RS22740 at 4738.701 kb on + strand at 4738.704 kb on - strand at 4738.846 kb on - strand at 4738.853 kb on + strand at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.078 kb on - strand, within BWI76_RS22745 at 4739.078 kb on - strand, within BWI76_RS22745 at 4739.078 kb on - strand at 4739.078 kb on - strand, within BWI76_RS22745 at 4739.078 kb on - strand, within BWI76_RS22745 at 4739.159 kb on - strand, within BWI76_RS22745
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 4,736,183 - BWI76_RS22725 0.72 -0.9 4,736,187 + BWI76_RS22725 0.72 -0.2 4,736,207 + BWI76_RS22725 0.75 +0.4 4,736,329 - -2.9 4,736,362 + -1.3 4,736,370 - +0.6 4,736,462 - BWI76_RS22730 0.15 -0.8 4,736,463 - BWI76_RS22730 0.15 +0.4 4,736,574 + BWI76_RS22730 0.30 +0.3 4,736,680 - BWI76_RS22730 0.44 +1.1 4,736,752 + BWI76_RS22730 0.54 -0.6 4,736,760 - BWI76_RS22730 0.55 -0.4 4,736,784 + BWI76_RS22730 0.58 +0.1 4,736,789 + BWI76_RS22730 0.59 +0.2 4,736,813 - BWI76_RS22730 0.62 -0.1 4,736,885 + BWI76_RS22730 0.72 -1.8 4,736,909 - BWI76_RS22730 0.75 +0.8 4,736,990 - BWI76_RS22730 0.86 -1.2 4,737,010 - BWI76_RS22730 0.88 -0.5 4,737,129 + -2.1 4,737,166 - +0.3 4,737,215 + BWI76_RS22735 0.10 -0.8 4,737,215 + BWI76_RS22735 0.10 -0.7 4,737,215 + BWI76_RS22735 0.10 -0.8 4,737,478 + BWI76_RS22735 0.35 -1.2 4,737,602 + BWI76_RS22735 0.47 -1.3 4,737,610 - +5.3 4,737,806 - BWI76_RS22735 0.66 -0.7 4,737,956 + BWI76_RS22735 0.80 -0.2 4,738,074 - -0.3 4,738,243 - BWI76_RS22740 0.12 +0.3 4,738,264 + BWI76_RS22740 0.16 +0.8 4,738,359 + BWI76_RS22740 0.33 +0.1 4,738,359 + BWI76_RS22740 0.33 -0.0 4,738,367 - BWI76_RS22740 0.34 -0.4 4,738,367 - BWI76_RS22740 0.34 +0.3 4,738,431 - BWI76_RS22740 0.46 -1.0 4,738,494 + BWI76_RS22740 0.57 -0.2 4,738,549 + BWI76_RS22740 0.67 +0.3 4,738,701 + -0.6 4,738,704 - +1.7 4,738,846 - -0.9 4,738,853 + +0.4 4,739,070 + BWI76_RS22745 0.22 +0.3 4,739,070 + BWI76_RS22745 0.22 +1.1 4,739,070 + BWI76_RS22745 0.22 -0.0 4,739,070 + -1.2 4,739,070 + BWI76_RS22745 0.22 -1.2 4,739,070 + BWI76_RS22745 0.22 -2.2 4,739,070 + BWI76_RS22745 0.22 +1.2 4,739,078 - BWI76_RS22745 0.23 -0.3 4,739,078 - BWI76_RS22745 0.23 -3.5 4,739,078 - +2.4 4,739,078 - BWI76_RS22745 0.23 -0.3 4,739,078 - BWI76_RS22745 0.23 +0.9 4,739,159 - BWI76_RS22745 0.28 -0.3
Or see this region's nucleotide sequence