Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21170 and BWI76_RS21175 are separated by 120 nucleotides BWI76_RS21175 and BWI76_RS21180 are separated by 54 nucleotides BWI76_RS21180 and BWI76_RS21185 overlap by 10 nucleotides BWI76_RS21185 and BWI76_RS21190 are separated by 124 nucleotides
BWI76_RS21170: BWI76_RS21170 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 4,432,173 to 4,432,904
_RS21170
BWI76_RS21175: BWI76_RS21175 - inositol monophosphatase, at 4,433,025 to 4,433,828
_RS21175
BWI76_RS21180: BWI76_RS21180 - nickel transporter, at 4,433,883 to 4,434,863
_RS21180
BWI76_RS21185: BWI76_RS21185 - hypothetical protein, at 4,434,854 to 4,435,492
_RS21185
BWI76_RS21190: BWI76_RS21190 - stationary phase inducible protein CsiE, at 4,435,617 to 4,436,894
_RS21190
Position (kb)
4433
4434
4435 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4434.142 kb on + strand, within BWI76_RS21180 at 4434.207 kb on + strand, within BWI76_RS21180 at 4434.213 kb on - strand, within BWI76_RS21180 at 4434.461 kb on + strand, within BWI76_RS21180 at 4434.484 kb on - strand, within BWI76_RS21180 at 4434.484 kb on - strand, within BWI76_RS21180 at 4434.509 kb on - strand, within BWI76_RS21180 at 4434.588 kb on + strand, within BWI76_RS21180 at 4434.706 kb on - strand, within BWI76_RS21180 at 4434.898 kb on + strand at 4435.053 kb on + strand, within BWI76_RS21185 at 4435.066 kb on + strand, within BWI76_RS21185 at 4435.126 kb on + strand, within BWI76_RS21185 at 4435.134 kb on - strand, within BWI76_RS21185 at 4435.141 kb on - strand, within BWI76_RS21185 at 4435.311 kb on + strand, within BWI76_RS21185 at 4435.329 kb on - strand, within BWI76_RS21185 at 4435.493 kb on - strand at 4435.677 kb on + strand at 4435.747 kb on - strand, within BWI76_RS21190 at 4435.758 kb on + strand, within BWI76_RS21190
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 4,434,142 + BWI76_RS21180 0.26 -1.9 4,434,207 + BWI76_RS21180 0.33 -0.7 4,434,213 - BWI76_RS21180 0.34 -0.2 4,434,461 + BWI76_RS21180 0.59 +0.7 4,434,484 - BWI76_RS21180 0.61 -1.0 4,434,484 - BWI76_RS21180 0.61 -1.0 4,434,509 - BWI76_RS21180 0.64 -2.0 4,434,588 + BWI76_RS21180 0.72 -0.5 4,434,706 - BWI76_RS21180 0.84 -0.2 4,434,898 + +1.1 4,435,053 + BWI76_RS21185 0.31 -0.9 4,435,066 + BWI76_RS21185 0.33 +1.0 4,435,126 + BWI76_RS21185 0.43 -1.6 4,435,134 - BWI76_RS21185 0.44 +0.2 4,435,141 - BWI76_RS21185 0.45 -3.3 4,435,311 + BWI76_RS21185 0.72 +0.3 4,435,329 - BWI76_RS21185 0.74 +1.1 4,435,493 - +1.0 4,435,677 + -0.2 4,435,747 - BWI76_RS21190 0.10 +0.7 4,435,758 + BWI76_RS21190 0.11 -0.4
Or see this region's nucleotide sequence