Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20275 and BWI76_RS20280 are separated by 58 nucleotides BWI76_RS20280 and BWI76_RS20285 are separated by 56 nucleotides BWI76_RS20285 and BWI76_RS20290 are separated by 208 nucleotides BWI76_RS20290 and BWI76_RS20295 are separated by 65 nucleotides
BWI76_RS20275: BWI76_RS20275 - NUDIX hydrolase, at 4,249,086 to 4,249,640
_RS20275
BWI76_RS20280: BWI76_RS20280 - phosphodiesterase, at 4,249,699 to 4,250,250
_RS20280
BWI76_RS20285: BWI76_RS20285 - glutathione S-transferase, at 4,250,307 to 4,250,951
_RS20285
BWI76_RS20290: BWI76_RS20290 - thiol:disulfide oxidoreductase, at 4,251,160 to 4,251,789
_RS20290
BWI76_RS20295: BWI76_RS20295 - dihydroneopterin triphosphate 2'-epimerase, at 4,251,855 to 4,252,217
_RS20295
Position (kb) 4250
4251 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4249.402 kb on - strand, within BWI76_RS20275 at 4249.673 kb on - strand at 4249.776 kb on + strand, within BWI76_RS20280 at 4249.885 kb on - strand, within BWI76_RS20280 at 4249.922 kb on + strand, within BWI76_RS20280 at 4250.187 kb on + strand, within BWI76_RS20280 at 4250.187 kb on + strand, within BWI76_RS20280 at 4250.226 kb on - strand at 4250.372 kb on - strand, within BWI76_RS20285 at 4250.639 kb on - strand, within BWI76_RS20285 at 4250.849 kb on + strand, within BWI76_RS20285 at 4250.946 kb on + strand at 4251.047 kb on + strand at 4251.055 kb on - strand at 4251.062 kb on - strand at 4251.082 kb on + strand at 4251.261 kb on - strand, within BWI76_RS20290 at 4251.364 kb on - strand, within BWI76_RS20290 at 4251.451 kb on - strand, within BWI76_RS20290 at 4251.611 kb on + strand, within BWI76_RS20290 at 4251.715 kb on + strand, within BWI76_RS20290 at 4251.715 kb on + strand, within BWI76_RS20290 at 4251.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 4,249,402 - BWI76_RS20275 0.57 -2.3 4,249,673 - -2.6 4,249,776 + BWI76_RS20280 0.14 +0.6 4,249,885 - BWI76_RS20280 0.34 -3.1 4,249,922 + BWI76_RS20280 0.40 +1.0 4,250,187 + BWI76_RS20280 0.88 -0.0 4,250,187 + BWI76_RS20280 0.88 +0.4 4,250,226 - +1.1 4,250,372 - BWI76_RS20285 0.10 -2.3 4,250,639 - BWI76_RS20285 0.51 -0.2 4,250,849 + BWI76_RS20285 0.84 -0.7 4,250,946 + -1.9 4,251,047 + +1.4 4,251,055 - -1.0 4,251,062 - -2.1 4,251,082 + -0.8 4,251,261 - BWI76_RS20290 0.16 -1.0 4,251,364 - BWI76_RS20290 0.32 +1.4 4,251,451 - BWI76_RS20290 0.46 -0.2 4,251,611 + BWI76_RS20290 0.72 +0.0 4,251,715 + BWI76_RS20290 0.88 +0.3 4,251,715 + BWI76_RS20290 0.88 +0.2 4,251,847 + -1.1
Or see this region's nucleotide sequence