Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20040

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20035 and BWI76_RS20040 are separated by 15 nucleotidesBWI76_RS20040 and BWI76_RS20045 are separated by 11 nucleotides BWI76_RS20035: BWI76_RS20035 - hypothetical protein, at 4,197,551 to 4,198,531 _RS20035 BWI76_RS20040: BWI76_RS20040 - tricarboxylic transport membrane protein, at 4,198,547 to 4,198,978 _RS20040 BWI76_RS20045: BWI76_RS20045 - hypothetical protein, at 4,198,990 to 4,200,510 _RS20045 Position (kb) 4198 4199Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4197.603 kb on - strandat 4197.610 kb on + strandat 4197.613 kb on - strandat 4197.705 kb on + strand, within BWI76_RS20035at 4197.852 kb on + strand, within BWI76_RS20035at 4197.852 kb on + strand, within BWI76_RS20035at 4198.263 kb on + strand, within BWI76_RS20035at 4198.393 kb on + strand, within BWI76_RS20035at 4198.677 kb on + strand, within BWI76_RS20040at 4198.697 kb on + strand, within BWI76_RS20040at 4198.737 kb on - strand, within BWI76_RS20040at 4198.909 kb on - strand, within BWI76_RS20040at 4199.204 kb on - strand, within BWI76_RS20045at 4199.237 kb on + strand, within BWI76_RS20045at 4199.343 kb on - strand, within BWI76_RS20045at 4199.825 kb on + strand, within BWI76_RS20045

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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4,197,603 - +1.0
4,197,610 + -0.5
4,197,613 - +0.5
4,197,705 + BWI76_RS20035 0.16 -1.7
4,197,852 + BWI76_RS20035 0.31 +1.1
4,197,852 + BWI76_RS20035 0.31 +0.2
4,198,263 + BWI76_RS20035 0.73 +0.2
4,198,393 + BWI76_RS20035 0.86 -3.9
4,198,677 + BWI76_RS20040 0.30 -0.4
4,198,697 + BWI76_RS20040 0.35 -0.2
4,198,737 - BWI76_RS20040 0.44 -1.6
4,198,909 - BWI76_RS20040 0.84 -3.5
4,199,204 - BWI76_RS20045 0.14 -1.0
4,199,237 + BWI76_RS20045 0.16 +1.4
4,199,343 - BWI76_RS20045 0.23 +0.4
4,199,825 + BWI76_RS20045 0.55 -1.0

Or see this region's nucleotide sequence