Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18600 and BWI76_RS18605 overlap by 11 nucleotides BWI76_RS18605 and BWI76_RS18610 are separated by 178 nucleotides BWI76_RS18610 and BWI76_RS18615 are separated by 213 nucleotides BWI76_RS18615 and BWI76_RS18620 are separated by 102 nucleotides
BWI76_RS18600: BWI76_RS18600 - HupC/HyaC/HydC family protein, at 3,857,352 to 3,857,960
_RS18600
BWI76_RS18605: BWI76_RS18605 - oxidoreductase molybdopterin-binding protein, at 3,857,950 to 3,858,717
_RS18605
BWI76_RS18610: BWI76_RS18610 - transcriptional regulator LysR, at 3,858,896 to 3,859,882
_RS18610
BWI76_RS18615: BWI76_RS18615 - glutathione S-transferase domain protein, at 3,860,096 to 3,860,704
_RS18615
BWI76_RS18620: BWI76_RS18620 - L,D-transpeptidase, at 3,860,807 to 3,861,748
_RS18620
Position (kb)
3858
3859
3860 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3858.065 kb on + strand, within BWI76_RS18605 at 3858.180 kb on + strand, within BWI76_RS18605 at 3858.189 kb on - strand, within BWI76_RS18605 at 3858.685 kb on + strand at 3859.038 kb on + strand, within BWI76_RS18610 at 3859.334 kb on + strand, within BWI76_RS18610 at 3859.580 kb on - strand, within BWI76_RS18610 at 3859.677 kb on - strand, within BWI76_RS18610 at 3859.972 kb on + strand at 3860.006 kb on + strand at 3860.006 kb on + strand at 3860.014 kb on + strand at 3860.016 kb on - strand at 3860.038 kb on - strand at 3860.338 kb on + strand, within BWI76_RS18615 at 3860.362 kb on + strand, within BWI76_RS18615 at 3860.387 kb on - strand, within BWI76_RS18615 at 3860.477 kb on - strand, within BWI76_RS18615 at 3860.492 kb on - strand, within BWI76_RS18615 at 3860.520 kb on - strand, within BWI76_RS18615 at 3860.558 kb on - strand, within BWI76_RS18615 at 3860.560 kb on + strand, within BWI76_RS18615 at 3860.617 kb on + strand at 3860.705 kb on + strand at 3860.713 kb on - strand at 3860.757 kb on - strand at 3860.867 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,858,065 + BWI76_RS18605 0.15 -2.4 3,858,180 + BWI76_RS18605 0.30 -1.9 3,858,189 - BWI76_RS18605 0.31 -2.1 3,858,685 + -1.8 3,859,038 + BWI76_RS18610 0.14 -2.8 3,859,334 + BWI76_RS18610 0.44 -3.4 3,859,580 - BWI76_RS18610 0.69 -3.5 3,859,677 - BWI76_RS18610 0.79 -0.5 3,859,972 + -2.2 3,860,006 + -0.5 3,860,006 + +0.5 3,860,014 + +0.2 3,860,016 - -3.1 3,860,038 - -0.2 3,860,338 + BWI76_RS18615 0.40 -0.7 3,860,362 + BWI76_RS18615 0.44 -2.6 3,860,387 - BWI76_RS18615 0.48 +1.7 3,860,477 - BWI76_RS18615 0.63 +0.0 3,860,492 - BWI76_RS18615 0.65 -0.6 3,860,520 - BWI76_RS18615 0.70 -1.5 3,860,558 - BWI76_RS18615 0.76 -0.7 3,860,560 + BWI76_RS18615 0.76 -1.1 3,860,617 + +0.3 3,860,705 + -0.2 3,860,713 - +0.2 3,860,757 - -0.0 3,860,867 + +1.8
Or see this region's nucleotide sequence