Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18575

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18560 and BWI76_RS18565 are separated by 53 nucleotidesBWI76_RS18565 and BWI76_RS18570 are separated by 0 nucleotidesBWI76_RS18570 and BWI76_RS18575 are separated by 193 nucleotidesBWI76_RS18575 and BWI76_RS18580 are separated by 4 nucleotidesBWI76_RS18580 and BWI76_RS18585 are separated by 383 nucleotides BWI76_RS18560: BWI76_RS18560 - tRNA-Asn, at 3,852,581 to 3,852,656 _RS18560 BWI76_RS18565: BWI76_RS18565 - pemk protein, at 3,852,710 to 3,853,043 _RS18565 BWI76_RS18570: BWI76_RS18570 - hypothetical protein, at 3,853,044 to 3,853,247 _RS18570 BWI76_RS18575: BWI76_RS18575 - transcriptional regulator, at 3,853,441 to 3,853,743 _RS18575 BWI76_RS18580: BWI76_RS18580 - hypothetical protein, at 3,853,748 to 3,854,101 _RS18580 BWI76_RS18585: BWI76_RS18585 - two-component sensor histidine kinase, at 3,854,485 to 3,855,903 _RS18585 Position (kb) 3853 3854Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3852.505 kb on + strandat 3852.870 kb on + strand, within BWI76_RS18565at 3852.971 kb on - strand, within BWI76_RS18565at 3853.049 kb on - strandat 3853.231 kb on - strandat 3853.249 kb on + strandat 3853.262 kb on - strandat 3853.346 kb on + strandat 3853.379 kb on + strandat 3853.414 kb on + strandat 3853.515 kb on - strand, within BWI76_RS18575at 3853.655 kb on - strand, within BWI76_RS18575at 3853.776 kb on - strandat 3853.805 kb on + strand, within BWI76_RS18580at 3854.056 kb on + strand, within BWI76_RS18580at 3854.308 kb on - strandat 3854.358 kb on + strandat 3854.490 kb on - strandat 3854.619 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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3,852,505 + -0.8
3,852,870 + BWI76_RS18565 0.48 -1.9
3,852,971 - BWI76_RS18565 0.78 -1.1
3,853,049 - -3.7
3,853,231 - +2.1
3,853,249 + +0.3
3,853,262 - -4.2
3,853,346 + -0.7
3,853,379 + -0.3
3,853,414 + -0.6
3,853,515 - BWI76_RS18575 0.24 -0.9
3,853,655 - BWI76_RS18575 0.71 -0.4
3,853,776 - -2.7
3,853,805 + BWI76_RS18580 0.16 -0.2
3,854,056 + BWI76_RS18580 0.87 +1.0
3,854,308 - -2.8
3,854,358 + -0.9
3,854,490 - +0.7
3,854,619 + +0.1

Or see this region's nucleotide sequence