Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17220

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17210 and BWI76_RS17215 are separated by 67 nucleotidesBWI76_RS17215 and BWI76_RS17220 are separated by 45 nucleotidesBWI76_RS17220 and BWI76_RS17225 overlap by 4 nucleotides BWI76_RS17210: BWI76_RS17210 - dsDNA-mimic protein, at 3,565,433 to 3,565,762 _RS17210 BWI76_RS17215: BWI76_RS17215 - transporter, at 3,565,830 to 3,566,667 _RS17215 BWI76_RS17220: BWI76_RS17220 - oligopeptide ABC transporter ATP-binding protein OppF, at 3,566,713 to 3,567,717 _RS17220 BWI76_RS17225: BWI76_RS17225 - oligopeptide ABC transporter ATP-binding protein OppD, at 3,567,714 to 3,568,727 _RS17225 Position (kb) 3566 3567 3568Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3565.735 kb on - strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.798 kb on - strandat 3565.841 kb on + strandat 3565.908 kb on + strandat 3565.916 kb on + strand, within BWI76_RS17215at 3565.916 kb on + strand, within BWI76_RS17215at 3565.936 kb on + strand, within BWI76_RS17215at 3566.006 kb on - strand, within BWI76_RS17215at 3566.034 kb on - strand, within BWI76_RS17215at 3566.197 kb on + strand, within BWI76_RS17215at 3566.198 kb on - strand, within BWI76_RS17215at 3566.207 kb on + strand, within BWI76_RS17215at 3566.210 kb on - strand, within BWI76_RS17215at 3566.215 kb on - strand, within BWI76_RS17215at 3566.220 kb on - strand, within BWI76_RS17215at 3566.244 kb on + strand, within BWI76_RS17215at 3566.259 kb on + strand, within BWI76_RS17215at 3566.321 kb on - strand, within BWI76_RS17215at 3566.323 kb on + strand, within BWI76_RS17215at 3566.325 kb on + strand, within BWI76_RS17215at 3566.326 kb on - strand, within BWI76_RS17215at 3566.345 kb on + strand, within BWI76_RS17215at 3566.433 kb on + strand, within BWI76_RS17215at 3566.441 kb on - strand, within BWI76_RS17215at 3566.469 kb on + strand, within BWI76_RS17215at 3566.477 kb on - strand, within BWI76_RS17215at 3566.560 kb on - strand, within BWI76_RS17215at 3566.560 kb on - strand, within BWI76_RS17215at 3566.560 kb on - strand, within BWI76_RS17215at 3566.562 kb on + strand, within BWI76_RS17215at 3566.700 kb on - strandat 3566.719 kb on - strandat 3566.923 kb on + strand, within BWI76_RS17220at 3566.924 kb on - strand, within BWI76_RS17220at 3566.943 kb on - strand, within BWI76_RS17220at 3567.198 kb on - strand, within BWI76_RS17220at 3567.491 kb on + strand, within BWI76_RS17220at 3567.585 kb on + strand, within BWI76_RS17220at 3567.778 kb on - strandat 3567.874 kb on + strand, within BWI76_RS17225at 3567.916 kb on + strand, within BWI76_RS17225at 3567.924 kb on - strand, within BWI76_RS17225at 3568.018 kb on - strand, within BWI76_RS17225at 3568.138 kb on + strand, within BWI76_RS17225at 3568.322 kb on - strand, within BWI76_RS17225at 3568.341 kb on - strand, within BWI76_RS17225at 3568.428 kb on - strand, within BWI76_RS17225at 3568.453 kb on - strand, within BWI76_RS17225

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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3,565,735 - -1.2
3,565,740 + -1.2
3,565,740 + +0.8
3,565,740 + +0.5
3,565,740 + +1.1
3,565,740 + +1.3
3,565,740 + +0.7
3,565,740 + +0.4
3,565,740 + -0.2
3,565,740 + -1.6
3,565,740 + +0.0
3,565,740 + +0.5
3,565,740 + +0.8
3,565,740 + +1.1
3,565,748 - -0.2
3,565,748 - -0.4
3,565,748 - -4.0
3,565,748 - -0.9
3,565,748 - +0.9
3,565,748 - -1.4
3,565,798 - -0.1
3,565,841 + -2.4
3,565,908 + +1.2
3,565,916 + BWI76_RS17215 0.10 +0.7
3,565,916 + BWI76_RS17215 0.10 -1.6
3,565,936 + BWI76_RS17215 0.13 -1.1
3,566,006 - BWI76_RS17215 0.21 -1.1
3,566,034 - BWI76_RS17215 0.24 -2.8
3,566,197 + BWI76_RS17215 0.44 +0.3
3,566,198 - BWI76_RS17215 0.44 -0.2
3,566,207 + BWI76_RS17215 0.45 +1.5
3,566,210 - BWI76_RS17215 0.45 +0.6
3,566,215 - BWI76_RS17215 0.46 -1.9
3,566,220 - BWI76_RS17215 0.47 -3.1
3,566,244 + BWI76_RS17215 0.49 +0.4
3,566,259 + BWI76_RS17215 0.51 +1.6
3,566,321 - BWI76_RS17215 0.59 +1.8
3,566,323 + BWI76_RS17215 0.59 +1.2
3,566,325 + BWI76_RS17215 0.59 -1.9
3,566,326 - BWI76_RS17215 0.59 +0.9
3,566,345 + BWI76_RS17215 0.61 +1.6
3,566,433 + BWI76_RS17215 0.72 -0.5
3,566,441 - BWI76_RS17215 0.73 -0.9
3,566,469 + BWI76_RS17215 0.76 +0.2
3,566,477 - BWI76_RS17215 0.77 +0.4
3,566,560 - BWI76_RS17215 0.87 +0.8
3,566,560 - BWI76_RS17215 0.87 +1.4
3,566,560 - BWI76_RS17215 0.87 -2.9
3,566,562 + BWI76_RS17215 0.87 -4.3
3,566,700 - +0.4
3,566,719 - +0.7
3,566,923 + BWI76_RS17220 0.21 +0.3
3,566,924 - BWI76_RS17220 0.21 -1.8
3,566,943 - BWI76_RS17220 0.23 -1.3
3,567,198 - BWI76_RS17220 0.48 -0.8
3,567,491 + BWI76_RS17220 0.77 -1.9
3,567,585 + BWI76_RS17220 0.87 -1.1
3,567,778 - -1.4
3,567,874 + BWI76_RS17225 0.16 +0.5
3,567,916 + BWI76_RS17225 0.20 -0.3
3,567,924 - BWI76_RS17225 0.21 +0.4
3,568,018 - BWI76_RS17225 0.30 -2.9
3,568,138 + BWI76_RS17225 0.42 +0.2
3,568,322 - BWI76_RS17225 0.60 -1.0
3,568,341 - BWI76_RS17225 0.62 +0.9
3,568,428 - BWI76_RS17225 0.70 +1.2
3,568,453 - BWI76_RS17225 0.73 +2.8

Or see this region's nucleotide sequence