Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17210 and BWI76_RS17215 are separated by 67 nucleotides BWI76_RS17215 and BWI76_RS17220 are separated by 45 nucleotides BWI76_RS17220 and BWI76_RS17225 overlap by 4 nucleotides
BWI76_RS17210: BWI76_RS17210 - dsDNA-mimic protein, at 3,565,433 to 3,565,762
_RS17210
BWI76_RS17215: BWI76_RS17215 - transporter, at 3,565,830 to 3,566,667
_RS17215
BWI76_RS17220: BWI76_RS17220 - oligopeptide ABC transporter ATP-binding protein OppF, at 3,566,713 to 3,567,717
_RS17220
BWI76_RS17225: BWI76_RS17225 - oligopeptide ABC transporter ATP-binding protein OppD, at 3,567,714 to 3,568,727
_RS17225
Position (kb)
3566
3567
3568 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3565.735 kb on - strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.798 kb on - strand at 3565.841 kb on + strand at 3565.908 kb on + strand at 3565.916 kb on + strand, within BWI76_RS17215 at 3565.916 kb on + strand, within BWI76_RS17215 at 3565.936 kb on + strand, within BWI76_RS17215 at 3566.006 kb on - strand, within BWI76_RS17215 at 3566.034 kb on - strand, within BWI76_RS17215 at 3566.197 kb on + strand, within BWI76_RS17215 at 3566.198 kb on - strand, within BWI76_RS17215 at 3566.207 kb on + strand, within BWI76_RS17215 at 3566.210 kb on - strand, within BWI76_RS17215 at 3566.215 kb on - strand, within BWI76_RS17215 at 3566.220 kb on - strand, within BWI76_RS17215 at 3566.244 kb on + strand, within BWI76_RS17215 at 3566.259 kb on + strand, within BWI76_RS17215 at 3566.321 kb on - strand, within BWI76_RS17215 at 3566.323 kb on + strand, within BWI76_RS17215 at 3566.325 kb on + strand, within BWI76_RS17215 at 3566.326 kb on - strand, within BWI76_RS17215 at 3566.345 kb on + strand, within BWI76_RS17215 at 3566.433 kb on + strand, within BWI76_RS17215 at 3566.441 kb on - strand, within BWI76_RS17215 at 3566.469 kb on + strand, within BWI76_RS17215 at 3566.477 kb on - strand, within BWI76_RS17215 at 3566.560 kb on - strand, within BWI76_RS17215 at 3566.560 kb on - strand, within BWI76_RS17215 at 3566.560 kb on - strand, within BWI76_RS17215 at 3566.562 kb on + strand, within BWI76_RS17215 at 3566.700 kb on - strand at 3566.719 kb on - strand at 3566.923 kb on + strand, within BWI76_RS17220 at 3566.924 kb on - strand, within BWI76_RS17220 at 3566.943 kb on - strand, within BWI76_RS17220 at 3567.198 kb on - strand, within BWI76_RS17220 at 3567.491 kb on + strand, within BWI76_RS17220 at 3567.585 kb on + strand, within BWI76_RS17220 at 3567.778 kb on - strand at 3567.874 kb on + strand, within BWI76_RS17225 at 3567.916 kb on + strand, within BWI76_RS17225 at 3567.924 kb on - strand, within BWI76_RS17225 at 3568.018 kb on - strand, within BWI76_RS17225 at 3568.138 kb on + strand, within BWI76_RS17225 at 3568.322 kb on - strand, within BWI76_RS17225 at 3568.341 kb on - strand, within BWI76_RS17225 at 3568.428 kb on - strand, within BWI76_RS17225 at 3568.453 kb on - strand, within BWI76_RS17225
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,565,735 - -1.2 3,565,740 + -1.2 3,565,740 + +0.8 3,565,740 + +0.5 3,565,740 + +1.1 3,565,740 + +1.3 3,565,740 + +0.7 3,565,740 + +0.4 3,565,740 + -0.2 3,565,740 + -1.6 3,565,740 + +0.0 3,565,740 + +0.5 3,565,740 + +0.8 3,565,740 + +1.1 3,565,748 - -0.2 3,565,748 - -0.4 3,565,748 - -4.0 3,565,748 - -0.9 3,565,748 - +0.9 3,565,748 - -1.4 3,565,798 - -0.1 3,565,841 + -2.4 3,565,908 + +1.2 3,565,916 + BWI76_RS17215 0.10 +0.7 3,565,916 + BWI76_RS17215 0.10 -1.6 3,565,936 + BWI76_RS17215 0.13 -1.1 3,566,006 - BWI76_RS17215 0.21 -1.1 3,566,034 - BWI76_RS17215 0.24 -2.8 3,566,197 + BWI76_RS17215 0.44 +0.3 3,566,198 - BWI76_RS17215 0.44 -0.2 3,566,207 + BWI76_RS17215 0.45 +1.5 3,566,210 - BWI76_RS17215 0.45 +0.6 3,566,215 - BWI76_RS17215 0.46 -1.9 3,566,220 - BWI76_RS17215 0.47 -3.1 3,566,244 + BWI76_RS17215 0.49 +0.4 3,566,259 + BWI76_RS17215 0.51 +1.6 3,566,321 - BWI76_RS17215 0.59 +1.8 3,566,323 + BWI76_RS17215 0.59 +1.2 3,566,325 + BWI76_RS17215 0.59 -1.9 3,566,326 - BWI76_RS17215 0.59 +0.9 3,566,345 + BWI76_RS17215 0.61 +1.6 3,566,433 + BWI76_RS17215 0.72 -0.5 3,566,441 - BWI76_RS17215 0.73 -0.9 3,566,469 + BWI76_RS17215 0.76 +0.2 3,566,477 - BWI76_RS17215 0.77 +0.4 3,566,560 - BWI76_RS17215 0.87 +0.8 3,566,560 - BWI76_RS17215 0.87 +1.4 3,566,560 - BWI76_RS17215 0.87 -2.9 3,566,562 + BWI76_RS17215 0.87 -4.3 3,566,700 - +0.4 3,566,719 - +0.7 3,566,923 + BWI76_RS17220 0.21 +0.3 3,566,924 - BWI76_RS17220 0.21 -1.8 3,566,943 - BWI76_RS17220 0.23 -1.3 3,567,198 - BWI76_RS17220 0.48 -0.8 3,567,491 + BWI76_RS17220 0.77 -1.9 3,567,585 + BWI76_RS17220 0.87 -1.1 3,567,778 - -1.4 3,567,874 + BWI76_RS17225 0.16 +0.5 3,567,916 + BWI76_RS17225 0.20 -0.3 3,567,924 - BWI76_RS17225 0.21 +0.4 3,568,018 - BWI76_RS17225 0.30 -2.9 3,568,138 + BWI76_RS17225 0.42 +0.2 3,568,322 - BWI76_RS17225 0.60 -1.0 3,568,341 - BWI76_RS17225 0.62 +0.9 3,568,428 - BWI76_RS17225 0.70 +1.2 3,568,453 - BWI76_RS17225 0.73 +2.8
Or see this region's nucleotide sequence