Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16775

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16770 and BWI76_RS16775 are separated by 497 nucleotidesBWI76_RS16775 and BWI76_RS16780 are separated by 237 nucleotides BWI76_RS16770: BWI76_RS16770 - extracellular solute-binding protein, at 3,474,697 to 3,475,785 _RS16770 BWI76_RS16775: BWI76_RS16775 - malate/lactate/ureidoglycolate dehydrogenase, at 3,476,283 to 3,477,365 _RS16775 BWI76_RS16780: BWI76_RS16780 - formate dehydrogenase subunit alpha, at 3,477,603 to 3,479,753 _RS16780 Position (kb) 3476 3477 3478Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3475.456 kb on + strand, within BWI76_RS16770at 3475.516 kb on - strand, within BWI76_RS16770at 3475.645 kb on + strand, within BWI76_RS16770at 3475.653 kb on - strand, within BWI76_RS16770at 3475.653 kb on - strand, within BWI76_RS16770at 3475.700 kb on + strandat 3475.706 kb on + strandat 3475.706 kb on + strandat 3475.714 kb on - strandat 3475.714 kb on - strandat 3475.716 kb on - strandat 3475.839 kb on + strandat 3475.847 kb on - strandat 3476.063 kb on - strandat 3476.064 kb on - strandat 3476.073 kb on + strandat 3476.083 kb on + strandat 3476.083 kb on + strandat 3476.083 kb on + strandat 3476.089 kb on + strandat 3476.090 kb on - strandat 3476.091 kb on - strandat 3476.097 kb on - strandat 3476.097 kb on - strandat 3476.204 kb on - strandat 3476.209 kb on + strandat 3476.209 kb on + strandat 3476.272 kb on + strandat 3476.314 kb on - strandat 3476.428 kb on + strand, within BWI76_RS16775at 3476.480 kb on + strand, within BWI76_RS16775at 3477.146 kb on + strand, within BWI76_RS16775at 3477.180 kb on - strand, within BWI76_RS16775at 3477.202 kb on + strand, within BWI76_RS16775at 3477.533 kb on + strandat 3477.556 kb on - strandat 3477.591 kb on + strandat 3477.620 kb on + strandat 3478.000 kb on - strand, within BWI76_RS16780at 3478.275 kb on + strand, within BWI76_RS16780at 3478.323 kb on + strand, within BWI76_RS16780at 3478.336 kb on - strand, within BWI76_RS16780

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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3,475,456 + BWI76_RS16770 0.70 -0.7
3,475,516 - BWI76_RS16770 0.75 +1.4
3,475,645 + BWI76_RS16770 0.87 -1.1
3,475,653 - BWI76_RS16770 0.88 +1.0
3,475,653 - BWI76_RS16770 0.88 +0.7
3,475,700 + -0.4
3,475,706 + -1.1
3,475,706 + -0.2
3,475,714 - -1.2
3,475,714 - +0.7
3,475,716 - +0.2
3,475,839 + -0.6
3,475,847 - +0.1
3,476,063 - -2.0
3,476,064 - +1.0
3,476,073 + -3.5
3,476,083 + +0.9
3,476,083 + -2.4
3,476,083 + +0.3
3,476,089 + +1.4
3,476,090 - +0.3
3,476,091 - +2.3
3,476,097 - +0.6
3,476,097 - -1.0
3,476,204 - +1.0
3,476,209 + -0.4
3,476,209 + -1.6
3,476,272 + -1.0
3,476,314 - -1.3
3,476,428 + BWI76_RS16775 0.13 +1.4
3,476,480 + BWI76_RS16775 0.18 -3.8
3,477,146 + BWI76_RS16775 0.80 -3.7
3,477,180 - BWI76_RS16775 0.83 -0.6
3,477,202 + BWI76_RS16775 0.85 -1.7
3,477,533 + +0.4
3,477,556 - +0.4
3,477,591 + -0.2
3,477,620 + +0.1
3,478,000 - BWI76_RS16780 0.18 -0.4
3,478,275 + BWI76_RS16780 0.31 +0.6
3,478,323 + BWI76_RS16780 0.33 +0.6
3,478,336 - BWI76_RS16780 0.34 -0.1

Or see this region's nucleotide sequence