Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16770 and BWI76_RS16775 are separated by 497 nucleotides BWI76_RS16775 and BWI76_RS16780 are separated by 237 nucleotides
BWI76_RS16770: BWI76_RS16770 - extracellular solute-binding protein, at 3,474,697 to 3,475,785
_RS16770
BWI76_RS16775: BWI76_RS16775 - malate/lactate/ureidoglycolate dehydrogenase, at 3,476,283 to 3,477,365
_RS16775
BWI76_RS16780: BWI76_RS16780 - formate dehydrogenase subunit alpha, at 3,477,603 to 3,479,753
_RS16780
Position (kb)
3476
3477
3478 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3475.456 kb on + strand, within BWI76_RS16770 at 3475.516 kb on - strand, within BWI76_RS16770 at 3475.645 kb on + strand, within BWI76_RS16770 at 3475.653 kb on - strand, within BWI76_RS16770 at 3475.653 kb on - strand, within BWI76_RS16770 at 3475.700 kb on + strand at 3475.706 kb on + strand at 3475.706 kb on + strand at 3475.714 kb on - strand at 3475.714 kb on - strand at 3475.716 kb on - strand at 3475.839 kb on + strand at 3475.847 kb on - strand at 3476.063 kb on - strand at 3476.064 kb on - strand at 3476.073 kb on + strand at 3476.083 kb on + strand at 3476.083 kb on + strand at 3476.083 kb on + strand at 3476.089 kb on + strand at 3476.090 kb on - strand at 3476.091 kb on - strand at 3476.097 kb on - strand at 3476.097 kb on - strand at 3476.204 kb on - strand at 3476.209 kb on + strand at 3476.209 kb on + strand at 3476.272 kb on + strand at 3476.314 kb on - strand at 3476.428 kb on + strand, within BWI76_RS16775 at 3476.480 kb on + strand, within BWI76_RS16775 at 3477.146 kb on + strand, within BWI76_RS16775 at 3477.180 kb on - strand, within BWI76_RS16775 at 3477.202 kb on + strand, within BWI76_RS16775 at 3477.533 kb on + strand at 3477.556 kb on - strand at 3477.591 kb on + strand at 3477.620 kb on + strand at 3478.000 kb on - strand, within BWI76_RS16780 at 3478.275 kb on + strand, within BWI76_RS16780 at 3478.323 kb on + strand, within BWI76_RS16780 at 3478.336 kb on - strand, within BWI76_RS16780
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,475,456 + BWI76_RS16770 0.70 -0.7 3,475,516 - BWI76_RS16770 0.75 +1.4 3,475,645 + BWI76_RS16770 0.87 -1.1 3,475,653 - BWI76_RS16770 0.88 +1.0 3,475,653 - BWI76_RS16770 0.88 +0.7 3,475,700 + -0.4 3,475,706 + -1.1 3,475,706 + -0.2 3,475,714 - -1.2 3,475,714 - +0.7 3,475,716 - +0.2 3,475,839 + -0.6 3,475,847 - +0.1 3,476,063 - -2.0 3,476,064 - +1.0 3,476,073 + -3.5 3,476,083 + +0.9 3,476,083 + -2.4 3,476,083 + +0.3 3,476,089 + +1.4 3,476,090 - +0.3 3,476,091 - +2.3 3,476,097 - +0.6 3,476,097 - -1.0 3,476,204 - +1.0 3,476,209 + -0.4 3,476,209 + -1.6 3,476,272 + -1.0 3,476,314 - -1.3 3,476,428 + BWI76_RS16775 0.13 +1.4 3,476,480 + BWI76_RS16775 0.18 -3.8 3,477,146 + BWI76_RS16775 0.80 -3.7 3,477,180 - BWI76_RS16775 0.83 -0.6 3,477,202 + BWI76_RS16775 0.85 -1.7 3,477,533 + +0.4 3,477,556 - +0.4 3,477,591 + -0.2 3,477,620 + +0.1 3,478,000 - BWI76_RS16780 0.18 -0.4 3,478,275 + BWI76_RS16780 0.31 +0.6 3,478,323 + BWI76_RS16780 0.33 +0.6 3,478,336 - BWI76_RS16780 0.34 -0.1
Or see this region's nucleotide sequence