Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16650 and BWI76_RS16655 are separated by 30 nucleotides BWI76_RS16655 and BWI76_RS16660 are separated by 308 nucleotides BWI76_RS16660 and BWI76_RS16665 are separated by 1 nucleotides BWI76_RS16665 and BWI76_RS16670 are separated by 22 nucleotides
BWI76_RS16650: BWI76_RS16650 - glyoxalase I, nickel isomerase, at 3,446,577 to 3,446,960
_RS16650
BWI76_RS16655: BWI76_RS16655 - CatA-like O-acetyltransferase, at 3,446,991 to 3,447,630
_RS16655
BWI76_RS16660: BWI76_RS16660 - SfnB family sulfur acquisition oxidoreductase, at 3,447,939 to 3,449,162
_RS16660
BWI76_RS16665: BWI76_RS16665 - ABC transporter permease, at 3,449,164 to 3,449,838
_RS16665
BWI76_RS16670: BWI76_RS16670 - NLPA lipoprotein, at 3,449,861 to 3,450,637
_RS16670
Position (kb)
3447
3448
3449
3450 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3446.960 kb on + strand at 3446.981 kb on + strand at 3447.073 kb on + strand, within BWI76_RS16655 at 3447.131 kb on - strand, within BWI76_RS16655 at 3447.451 kb on - strand, within BWI76_RS16655 at 3447.463 kb on - strand, within BWI76_RS16655 at 3447.493 kb on - strand, within BWI76_RS16655 at 3447.735 kb on - strand at 3448.498 kb on - strand, within BWI76_RS16660 at 3448.626 kb on - strand, within BWI76_RS16660 at 3448.819 kb on + strand, within BWI76_RS16660 at 3448.958 kb on + strand, within BWI76_RS16660 at 3449.066 kb on + strand at 3449.513 kb on - strand, within BWI76_RS16665 at 3449.537 kb on + strand, within BWI76_RS16665 at 3449.538 kb on - strand, within BWI76_RS16665 at 3449.712 kb on - strand, within BWI76_RS16665 at 3449.880 kb on + strand at 3450.033 kb on + strand, within BWI76_RS16670 at 3450.041 kb on - strand, within BWI76_RS16670 at 3450.124 kb on - strand, within BWI76_RS16670
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,446,960 + -0.1 3,446,981 + -0.1 3,447,073 + BWI76_RS16655 0.13 -1.3 3,447,131 - BWI76_RS16655 0.22 -2.9 3,447,451 - BWI76_RS16655 0.72 -1.1 3,447,463 - BWI76_RS16655 0.74 -0.9 3,447,493 - BWI76_RS16655 0.78 +0.1 3,447,735 - +0.9 3,448,498 - BWI76_RS16660 0.46 -3.4 3,448,626 - BWI76_RS16660 0.56 +0.5 3,448,819 + BWI76_RS16660 0.72 -2.1 3,448,958 + BWI76_RS16660 0.83 -2.4 3,449,066 + -0.7 3,449,513 - BWI76_RS16665 0.52 -0.2 3,449,537 + BWI76_RS16665 0.55 +0.5 3,449,538 - BWI76_RS16665 0.55 +0.3 3,449,712 - BWI76_RS16665 0.81 +1.0 3,449,880 + +2.0 3,450,033 + BWI76_RS16670 0.22 +0.6 3,450,041 - BWI76_RS16670 0.23 +0.5 3,450,124 - BWI76_RS16670 0.34 +0.3
Or see this region's nucleotide sequence