Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16590 and BWI76_RS16595 are separated by 24 nucleotides BWI76_RS16595 and BWI76_RS16600 are separated by 12 nucleotides BWI76_RS16600 and BWI76_RS16605 are separated by 9 nucleotides
BWI76_RS16590: BWI76_RS16590 - short-chain dehydrogenase, at 3,436,679 to 3,437,668
_RS16590
BWI76_RS16595: BWI76_RS16595 - hypothetical protein, at 3,437,693 to 3,438,211
_RS16595
BWI76_RS16600: BWI76_RS16600 - DUF421 domain-containing protein, at 3,438,224 to 3,438,670
_RS16600
BWI76_RS16605: BWI76_RS16605 - hypothetical protein, at 3,438,680 to 3,439,183
_RS16605
Position (kb)
3437
3438
3439 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3436.971 kb on - strand, within BWI76_RS16590 at 3437.313 kb on + strand, within BWI76_RS16590 at 3437.617 kb on - strand at 3437.879 kb on - strand, within BWI76_RS16595 at 3437.913 kb on + strand, within BWI76_RS16595 at 3437.931 kb on + strand, within BWI76_RS16595 at 3438.022 kb on + strand, within BWI76_RS16595 at 3438.022 kb on + strand, within BWI76_RS16595 at 3438.091 kb on + strand, within BWI76_RS16595 at 3438.091 kb on + strand, within BWI76_RS16595 at 3438.099 kb on - strand, within BWI76_RS16595 at 3438.110 kb on - strand, within BWI76_RS16595 at 3438.191 kb on + strand at 3438.198 kb on - strand at 3438.212 kb on + strand at 3438.256 kb on - strand at 3438.266 kb on - strand at 3438.266 kb on - strand at 3438.302 kb on + strand, within BWI76_RS16600 at 3438.313 kb on + strand, within BWI76_RS16600 at 3438.349 kb on - strand, within BWI76_RS16600 at 3438.393 kb on + strand, within BWI76_RS16600 at 3438.400 kb on - strand, within BWI76_RS16600 at 3438.474 kb on + strand, within BWI76_RS16600 at 3438.493 kb on + strand, within BWI76_RS16600 at 3438.493 kb on + strand, within BWI76_RS16600 at 3438.493 kb on + strand, within BWI76_RS16600 at 3438.493 kb on + strand, within BWI76_RS16600 at 3438.494 kb on + strand, within BWI76_RS16600 at 3438.501 kb on - strand, within BWI76_RS16600 at 3438.536 kb on + strand, within BWI76_RS16600 at 3438.571 kb on + strand at 3438.571 kb on + strand, within BWI76_RS16600 at 3438.571 kb on + strand, within BWI76_RS16600 at 3438.571 kb on + strand, within BWI76_RS16600 at 3438.571 kb on + strand, within BWI76_RS16600 at 3438.578 kb on + strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.593 kb on - strand, within BWI76_RS16600 at 3438.668 kb on - strand at 3438.715 kb on - strand at 3438.720 kb on + strand at 3438.763 kb on + strand, within BWI76_RS16605 at 3438.768 kb on + strand at 3438.771 kb on - strand at 3438.771 kb on - strand at 3438.840 kb on - strand, within BWI76_RS16605 at 3438.853 kb on - strand, within BWI76_RS16605 at 3438.863 kb on - strand, within BWI76_RS16605 at 3438.870 kb on - strand, within BWI76_RS16605 at 3438.870 kb on - strand, within BWI76_RS16605 at 3438.870 kb on - strand, within BWI76_RS16605 at 3438.874 kb on - strand, within BWI76_RS16605 at 3438.885 kb on - strand, within BWI76_RS16605 at 3438.885 kb on - strand, within BWI76_RS16605 at 3438.885 kb on - strand, within BWI76_RS16605 at 3438.903 kb on - strand, within BWI76_RS16605 at 3438.943 kb on - strand, within BWI76_RS16605 at 3439.064 kb on - strand, within BWI76_RS16605 at 3439.147 kb on + strand at 3439.147 kb on + strand at 3439.147 kb on + strand at 3439.155 kb on - strand at 3439.155 kb on - strand at 3439.155 kb on - strand at 3439.155 kb on - strand at 3439.155 kb on - strand at 3439.155 kb on - strand at 3439.155 kb on - strand at 3439.182 kb on + strand at 3439.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,436,971 - BWI76_RS16590 0.29 +0.2 3,437,313 + BWI76_RS16590 0.64 -1.6 3,437,617 - -0.8 3,437,879 - BWI76_RS16595 0.36 +0.6 3,437,913 + BWI76_RS16595 0.42 -1.8 3,437,931 + BWI76_RS16595 0.46 -0.5 3,438,022 + BWI76_RS16595 0.63 -0.1 3,438,022 + BWI76_RS16595 0.63 -1.2 3,438,091 + BWI76_RS16595 0.77 -2.1 3,438,091 + BWI76_RS16595 0.77 -1.5 3,438,099 - BWI76_RS16595 0.78 -2.1 3,438,110 - BWI76_RS16595 0.80 -0.1 3,438,191 + +0.7 3,438,198 - +0.4 3,438,212 + -2.8 3,438,256 - +1.6 3,438,266 - -0.1 3,438,266 - -2.8 3,438,302 + BWI76_RS16600 0.17 -0.6 3,438,313 + BWI76_RS16600 0.20 +1.9 3,438,349 - BWI76_RS16600 0.28 +0.8 3,438,393 + BWI76_RS16600 0.38 -1.8 3,438,400 - BWI76_RS16600 0.39 -1.2 3,438,474 + BWI76_RS16600 0.56 -0.2 3,438,493 + BWI76_RS16600 0.60 -0.6 3,438,493 + BWI76_RS16600 0.60 +0.4 3,438,493 + BWI76_RS16600 0.60 +0.9 3,438,493 + BWI76_RS16600 0.60 +0.3 3,438,494 + BWI76_RS16600 0.60 +0.7 3,438,501 - BWI76_RS16600 0.62 +0.1 3,438,536 + BWI76_RS16600 0.70 -0.1 3,438,571 + -0.9 3,438,571 + BWI76_RS16600 0.78 -2.3 3,438,571 + BWI76_RS16600 0.78 -3.0 3,438,571 + BWI76_RS16600 0.78 +1.9 3,438,571 + BWI76_RS16600 0.78 +1.9 3,438,578 + BWI76_RS16600 0.79 -0.9 3,438,579 - BWI76_RS16600 0.79 -0.4 3,438,579 - BWI76_RS16600 0.79 +0.9 3,438,579 - BWI76_RS16600 0.79 +1.4 3,438,579 - BWI76_RS16600 0.79 -0.6 3,438,579 - BWI76_RS16600 0.79 +2.2 3,438,579 - BWI76_RS16600 0.79 -0.6 3,438,579 - BWI76_RS16600 0.79 +0.0 3,438,579 - BWI76_RS16600 0.79 +1.7 3,438,579 - BWI76_RS16600 0.79 -1.2 3,438,579 - BWI76_RS16600 0.79 -1.6 3,438,579 - BWI76_RS16600 0.79 +2.1 3,438,579 - BWI76_RS16600 0.79 +0.8 3,438,593 - BWI76_RS16600 0.83 -0.6 3,438,668 - +1.1 3,438,715 - -1.9 3,438,720 + +1.9 3,438,763 + BWI76_RS16605 0.16 +1.9 3,438,768 + +1.9 3,438,771 - +0.9 3,438,771 - +1.9 3,438,840 - BWI76_RS16605 0.32 -1.2 3,438,853 - BWI76_RS16605 0.34 +0.5 3,438,863 - BWI76_RS16605 0.36 -3.1 3,438,870 - BWI76_RS16605 0.38 +0.1 3,438,870 - BWI76_RS16605 0.38 +0.9 3,438,870 - BWI76_RS16605 0.38 +0.4 3,438,874 - BWI76_RS16605 0.38 +0.4 3,438,885 - BWI76_RS16605 0.41 +1.7 3,438,885 - BWI76_RS16605 0.41 -0.0 3,438,885 - BWI76_RS16605 0.41 -2.4 3,438,903 - BWI76_RS16605 0.44 -2.2 3,438,943 - BWI76_RS16605 0.52 +0.3 3,439,064 - BWI76_RS16605 0.76 -2.1 3,439,147 + -1.1 3,439,147 + -0.9 3,439,147 + +0.0 3,439,155 - -0.1 3,439,155 - -0.4 3,439,155 - -1.5 3,439,155 - +0.5 3,439,155 - +0.6 3,439,155 - -0.6 3,439,155 - -0.1 3,439,182 + +1.4 3,439,190 - -1.0
Or see this region's nucleotide sequence