Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16485

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16475 and BWI76_RS16480 are separated by 79 nucleotidesBWI76_RS16480 and BWI76_RS16485 are separated by 106 nucleotidesBWI76_RS16485 and BWI76_RS16490 are separated by 51 nucleotidesBWI76_RS16490 and BWI76_RS16495 are separated by 339 nucleotides BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193 _RS16475 BWI76_RS16480: BWI76_RS16480 - lactoylglutathione lyase, at 3,417,273 to 3,417,680 _RS16480 BWI76_RS16485: BWI76_RS16485 - ribonuclease T, at 3,417,787 to 3,418,431 _RS16485 BWI76_RS16490: BWI76_RS16490 - glutaredoxin, at 3,418,483 to 3,418,830 _RS16490 BWI76_RS16495: BWI76_RS16495 - endopeptidase, at 3,419,170 to 3,420,024 _RS16495 Position (kb) 3417 3418 3419Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3417.036 kb on + strand, within BWI76_RS16475at 3417.229 kb on + strandat 3417.237 kb on - strandat 3417.334 kb on + strand, within BWI76_RS16480at 3417.476 kb on + strand, within BWI76_RS16480at 3417.527 kb on - strand, within BWI76_RS16480at 3417.658 kb on + strandat 3417.804 kb on + strandat 3418.251 kb on - strand, within BWI76_RS16485at 3418.312 kb on + strand, within BWI76_RS16485at 3418.612 kb on - strand, within BWI76_RS16490at 3419.134 kb on + strandat 3419.134 kb on + strandat 3419.306 kb on - strand, within BWI76_RS16495

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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3,417,036 + BWI76_RS16475 0.86 +0.2
3,417,229 + +0.3
3,417,237 - -0.2
3,417,334 + BWI76_RS16480 0.15 -0.1
3,417,476 + BWI76_RS16480 0.50 +0.8
3,417,527 - BWI76_RS16480 0.62 -0.5
3,417,658 + +0.7
3,417,804 + -2.1
3,418,251 - BWI76_RS16485 0.72 -1.5
3,418,312 + BWI76_RS16485 0.81 -2.2
3,418,612 - BWI76_RS16490 0.37 +0.0
3,419,134 + +0.9
3,419,134 + +4.5
3,419,306 - BWI76_RS16495 0.16 -0.9

Or see this region's nucleotide sequence