Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16475 and BWI76_RS16480 are separated by 79 nucleotides BWI76_RS16480 and BWI76_RS16485 are separated by 106 nucleotides BWI76_RS16485 and BWI76_RS16490 are separated by 51 nucleotides BWI76_RS16490 and BWI76_RS16495 are separated by 339 nucleotides
BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193
_RS16475
BWI76_RS16480: BWI76_RS16480 - lactoylglutathione lyase, at 3,417,273 to 3,417,680
_RS16480
BWI76_RS16485: BWI76_RS16485 - ribonuclease T, at 3,417,787 to 3,418,431
_RS16485
BWI76_RS16490: BWI76_RS16490 - glutaredoxin, at 3,418,483 to 3,418,830
_RS16490
BWI76_RS16495: BWI76_RS16495 - endopeptidase, at 3,419,170 to 3,420,024
_RS16495
Position (kb)
3417
3418
3419 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3417.036 kb on + strand, within BWI76_RS16475 at 3417.229 kb on + strand at 3417.237 kb on - strand at 3417.334 kb on + strand, within BWI76_RS16480 at 3417.476 kb on + strand, within BWI76_RS16480 at 3417.527 kb on - strand, within BWI76_RS16480 at 3417.658 kb on + strand at 3417.804 kb on + strand at 3418.251 kb on - strand, within BWI76_RS16485 at 3418.312 kb on + strand, within BWI76_RS16485 at 3418.612 kb on - strand, within BWI76_RS16490 at 3419.134 kb on + strand at 3419.134 kb on + strand at 3419.306 kb on - strand, within BWI76_RS16495
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,417,036 + BWI76_RS16475 0.86 +0.2 3,417,229 + +0.3 3,417,237 - -0.2 3,417,334 + BWI76_RS16480 0.15 -0.1 3,417,476 + BWI76_RS16480 0.50 +0.8 3,417,527 - BWI76_RS16480 0.62 -0.5 3,417,658 + +0.7 3,417,804 + -2.1 3,418,251 - BWI76_RS16485 0.72 -1.5 3,418,312 + BWI76_RS16485 0.81 -2.2 3,418,612 - BWI76_RS16490 0.37 +0.0 3,419,134 + +0.9 3,419,134 + +4.5 3,419,306 - BWI76_RS16495 0.16 -0.9
Or see this region's nucleotide sequence