Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16050 and BWI76_RS16055 overlap by 4 nucleotides BWI76_RS16055 and BWI76_RS16060 overlap by 11 nucleotides BWI76_RS16060 and BWI76_RS16065 are separated by 215 nucleotides
BWI76_RS16050: BWI76_RS16050 - malonate decarboxylase holo-ACP synthase, at 3,327,649 to 3,328,269
_RS16050
BWI76_RS16055: BWI76_RS16055 - malonate decarboxylase subunit epsilon, at 3,328,266 to 3,329,168
_RS16055
BWI76_RS16060: BWI76_RS16060 - LysR family transcriptional regulator, at 3,329,158 to 3,330,084
_RS16060
BWI76_RS16065: BWI76_RS16065 - malate:quinone oxidoreductase, at 3,330,300 to 3,331,955
_RS16065
Position (kb)
3329
3330
3331 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3328.433 kb on - strand, within BWI76_RS16055 at 3328.607 kb on + strand, within BWI76_RS16055 at 3328.703 kb on + strand, within BWI76_RS16055 at 3328.905 kb on + strand, within BWI76_RS16055 at 3328.913 kb on - strand, within BWI76_RS16055 at 3328.942 kb on - strand, within BWI76_RS16055 at 3329.424 kb on + strand, within BWI76_RS16060 at 3329.432 kb on - strand, within BWI76_RS16060 at 3329.522 kb on + strand, within BWI76_RS16060 at 3329.564 kb on + strand, within BWI76_RS16060 at 3329.756 kb on - strand, within BWI76_RS16060 at 3329.948 kb on - strand at 3330.467 kb on + strand, within BWI76_RS16065 at 3330.538 kb on - strand, within BWI76_RS16065 at 3330.736 kb on - strand, within BWI76_RS16065 at 3330.745 kb on - strand, within BWI76_RS16065 at 3330.791 kb on + strand, within BWI76_RS16065 at 3330.793 kb on - strand, within BWI76_RS16065 at 3330.815 kb on + strand, within BWI76_RS16065 at 3330.830 kb on + strand, within BWI76_RS16065 at 3330.939 kb on - strand at 3331.006 kb on + strand, within BWI76_RS16065 at 3331.039 kb on + strand, within BWI76_RS16065 at 3331.047 kb on - strand, within BWI76_RS16065 at 3331.052 kb on - strand, within BWI76_RS16065 at 3331.056 kb on + strand, within BWI76_RS16065 at 3331.078 kb on - strand, within BWI76_RS16065
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,328,433 - BWI76_RS16055 0.18 -0.9 3,328,607 + BWI76_RS16055 0.38 -1.0 3,328,703 + BWI76_RS16055 0.48 -3.2 3,328,905 + BWI76_RS16055 0.71 +0.6 3,328,913 - BWI76_RS16055 0.72 -2.7 3,328,942 - BWI76_RS16055 0.75 -2.7 3,329,424 + BWI76_RS16060 0.29 -1.0 3,329,432 - BWI76_RS16060 0.30 -0.3 3,329,522 + BWI76_RS16060 0.39 +0.1 3,329,564 + BWI76_RS16060 0.44 -0.2 3,329,756 - BWI76_RS16060 0.65 +0.7 3,329,948 - +0.3 3,330,467 + BWI76_RS16065 0.10 -3.6 3,330,538 - BWI76_RS16065 0.14 +0.9 3,330,736 - BWI76_RS16065 0.26 -0.1 3,330,745 - BWI76_RS16065 0.27 +0.3 3,330,791 + BWI76_RS16065 0.30 -0.7 3,330,793 - BWI76_RS16065 0.30 -4.4 3,330,815 + BWI76_RS16065 0.31 -3.2 3,330,830 + BWI76_RS16065 0.32 -2.6 3,330,939 - +1.9 3,331,006 + BWI76_RS16065 0.43 -1.3 3,331,039 + BWI76_RS16065 0.45 -0.5 3,331,047 - BWI76_RS16065 0.45 -1.2 3,331,052 - BWI76_RS16065 0.45 +0.0 3,331,056 + BWI76_RS16065 0.46 -1.3 3,331,078 - BWI76_RS16065 0.47 -2.1
Or see this region's nucleotide sequence