Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15905 and BWI76_RS15910 are separated by 60 nucleotides BWI76_RS15910 and BWI76_RS15915 overlap by 7 nucleotides BWI76_RS15915 and BWI76_RS15920 are separated by 102 nucleotides BWI76_RS15920 and BWI76_RS15925 are separated by 148 nucleotides
BWI76_RS15905: BWI76_RS15905 - hypothetical protein, at 3,300,090 to 3,300,431
_RS15905
BWI76_RS15910: BWI76_RS15910 - spermidine acetyltransferase, at 3,300,492 to 3,301,052
_RS15910
BWI76_RS15915: BWI76_RS15915 - YnfC family lipoprotein, at 3,301,046 to 3,301,756
_RS15915
BWI76_RS15920: BWI76_RS15920 - hypothetical protein, at 3,301,859 to 3,302,110
_RS15920
BWI76_RS15925: BWI76_RS15925 - dimethyl sulfoxide reductase subunit A, at 3,302,259 to 3,304,694
_RS15925
Position (kb)
3301
3302 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3300.394 kb on + strand, within BWI76_RS15905 at 3300.402 kb on - strand at 3300.402 kb on - strand at 3300.545 kb on - strand at 3300.545 kb on - strand at 3300.847 kb on + strand, within BWI76_RS15910 at 3301.015 kb on - strand at 3301.038 kb on + strand at 3301.082 kb on - strand at 3301.114 kb on - strand at 3301.123 kb on + strand, within BWI76_RS15915 at 3301.234 kb on + strand, within BWI76_RS15915 at 3301.677 kb on + strand, within BWI76_RS15915 at 3301.689 kb on + strand at 3301.702 kb on + strand at 3301.779 kb on - strand at 3301.871 kb on + strand at 3301.887 kb on - strand, within BWI76_RS15920 at 3302.134 kb on - strand at 3302.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,300,394 + BWI76_RS15905 0.89 -0.7 3,300,402 - -0.1 3,300,402 - +2.0 3,300,545 - -3.9 3,300,545 - +0.1 3,300,847 + BWI76_RS15910 0.63 +0.3 3,301,015 - -4.1 3,301,038 + +1.9 3,301,082 - -0.4 3,301,114 - +1.2 3,301,123 + BWI76_RS15915 0.11 -0.9 3,301,234 + BWI76_RS15915 0.26 -1.3 3,301,677 + BWI76_RS15915 0.89 -0.9 3,301,689 + -0.9 3,301,702 + -3.3 3,301,779 - -0.6 3,301,871 + -1.1 3,301,887 - BWI76_RS15920 0.11 +0.6 3,302,134 - +0.2 3,302,473 - +0.4
Or see this region's nucleotide sequence