Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15720 and BWI76_RS15725 overlap by 1 nucleotides BWI76_RS15725 and BWI76_RS15730 are separated by 260 nucleotides BWI76_RS15730 and BWI76_RS15735 overlap by 8 nucleotides BWI76_RS15735 and BWI76_RS15740 overlap by 4 nucleotides BWI76_RS15740 and BWI76_RS15745 overlap by 1 nucleotides
BWI76_RS15720: BWI76_RS15720 - prophage protein NinE, at 3,279,351 to 3,279,521
_RS15720
BWI76_RS15725: BWI76_RS15725 - hypothetical protein, at 3,279,521 to 3,279,976
_RS15725
BWI76_RS15730: BWI76_RS15730 - hypothetical protein, at 3,280,237 to 3,280,467
_RS15730
BWI76_RS15735: BWI76_RS15735 - hypothetical protein, at 3,280,460 to 3,281,005
_RS15735
BWI76_RS15740: BWI76_RS15740 - HNH endonuclease, at 3,281,002 to 3,281,382
_RS15740
BWI76_RS15745: BWI76_RS15745 - hypothetical protein, at 3,281,382 to 3,282,116
_RS15745
Position (kb)
3280
3281
3282 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3279.550 kb on - strand at 3279.838 kb on + strand, within BWI76_RS15725 at 3279.846 kb on - strand, within BWI76_RS15725 at 3279.864 kb on + strand, within BWI76_RS15725 at 3279.949 kb on + strand at 3280.350 kb on - strand, within BWI76_RS15730 at 3280.459 kb on - strand at 3280.712 kb on + strand, within BWI76_RS15735 at 3280.717 kb on - strand, within BWI76_RS15735 at 3280.976 kb on - strand at 3281.124 kb on - strand, within BWI76_RS15740 at 3281.236 kb on + strand, within BWI76_RS15740 at 3281.238 kb on + strand, within BWI76_RS15740 at 3281.520 kb on - strand, within BWI76_RS15745 at 3281.692 kb on + strand, within BWI76_RS15745 at 3281.700 kb on - strand, within BWI76_RS15745 at 3281.917 kb on + strand, within BWI76_RS15745 at 3281.925 kb on - strand, within BWI76_RS15745
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,279,550 - +0.2 3,279,838 + BWI76_RS15725 0.70 +1.2 3,279,846 - BWI76_RS15725 0.71 -0.2 3,279,864 + BWI76_RS15725 0.75 -0.4 3,279,949 + -0.2 3,280,350 - BWI76_RS15730 0.49 +0.9 3,280,459 - -0.7 3,280,712 + BWI76_RS15735 0.46 -2.8 3,280,717 - BWI76_RS15735 0.47 -0.7 3,280,976 - -0.5 3,281,124 - BWI76_RS15740 0.32 -2.8 3,281,236 + BWI76_RS15740 0.61 -1.8 3,281,238 + BWI76_RS15740 0.62 -0.5 3,281,520 - BWI76_RS15745 0.19 -0.3 3,281,692 + BWI76_RS15745 0.42 -2.2 3,281,700 - BWI76_RS15745 0.43 -3.0 3,281,917 + BWI76_RS15745 0.73 +2.2 3,281,925 - BWI76_RS15745 0.74 -0.7
Or see this region's nucleotide sequence