Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15655 and BWI76_RS15660 overlap by 14 nucleotides BWI76_RS15660 and BWI76_RS15665 are separated by 31 nucleotides BWI76_RS15665 and BWI76_RS15670 are separated by 274 nucleotides BWI76_RS15670 and BWI76_RS15675 are separated by 69 nucleotides BWI76_RS15675 and BWI76_RS15680 are separated by 111 nucleotides
BWI76_RS15655: BWI76_RS15655 - hypothetical protein, at 3,271,852 to 3,273,330
_RS15655
BWI76_RS15660: BWI76_RS15660 - hypothetical protein, at 3,273,317 to 3,273,796
_RS15660
BWI76_RS15665: BWI76_RS15665 - hypothetical protein, at 3,273,828 to 3,274,475
_RS15665
BWI76_RS15670: BWI76_RS15670 - hypothetical protein, at 3,274,750 to 3,274,950
_RS15670
BWI76_RS15675: BWI76_RS15675 - hypothetical protein, at 3,275,020 to 3,275,265
_RS15675
BWI76_RS15680: BWI76_RS15680 - hypothetical protein, at 3,275,377 to 3,275,562
_RS15680
Position (kb)
3273
3274
3275 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3272.936 kb on + strand, within BWI76_RS15655 at 3272.993 kb on + strand, within BWI76_RS15655 at 3273.020 kb on - strand, within BWI76_RS15655 at 3273.088 kb on + strand, within BWI76_RS15655 at 3273.123 kb on + strand, within BWI76_RS15655 at 3273.196 kb on + strand at 3273.209 kb on - strand at 3273.415 kb on + strand, within BWI76_RS15660 at 3273.809 kb on + strand at 3273.817 kb on - strand at 3273.817 kb on - strand at 3273.867 kb on - strand at 3273.918 kb on - strand, within BWI76_RS15665 at 3274.043 kb on - strand, within BWI76_RS15665 at 3274.069 kb on + strand, within BWI76_RS15665 at 3274.327 kb on + strand, within BWI76_RS15665 at 3274.458 kb on + strand at 3274.487 kb on - strand at 3274.489 kb on - strand at 3274.494 kb on - strand at 3274.621 kb on + strand at 3274.867 kb on - strand, within BWI76_RS15670 at 3274.872 kb on + strand, within BWI76_RS15670 at 3274.872 kb on + strand, within BWI76_RS15670 at 3274.894 kb on - strand, within BWI76_RS15670 at 3275.005 kb on + strand at 3275.008 kb on - strand at 3275.165 kb on + strand, within BWI76_RS15675 at 3275.186 kb on + strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.221 kb on - strand, within BWI76_RS15675 at 3275.221 kb on - strand, within BWI76_RS15675 at 3275.278 kb on + strand at 3275.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,272,936 + BWI76_RS15655 0.73 +0.9 3,272,993 + BWI76_RS15655 0.77 -3.1 3,273,020 - BWI76_RS15655 0.79 +1.4 3,273,088 + BWI76_RS15655 0.84 +1.4 3,273,123 + BWI76_RS15655 0.86 -3.7 3,273,196 + +0.1 3,273,209 - -0.1 3,273,415 + BWI76_RS15660 0.20 +1.2 3,273,809 + -1.9 3,273,817 - -2.7 3,273,817 - -1.4 3,273,867 - -0.4 3,273,918 - BWI76_RS15665 0.14 -2.7 3,274,043 - BWI76_RS15665 0.33 -0.0 3,274,069 + BWI76_RS15665 0.37 -2.4 3,274,327 + BWI76_RS15665 0.77 -3.0 3,274,458 + +2.0 3,274,487 - +0.8 3,274,489 - -1.9 3,274,494 - -3.1 3,274,621 + -0.3 3,274,867 - BWI76_RS15670 0.58 -0.1 3,274,872 + BWI76_RS15670 0.61 +2.1 3,274,872 + BWI76_RS15670 0.61 -4.2 3,274,894 - BWI76_RS15670 0.72 -0.8 3,275,005 + -1.6 3,275,008 - -1.9 3,275,165 + BWI76_RS15675 0.59 -0.6 3,275,186 + BWI76_RS15675 0.67 +0.1 3,275,194 - BWI76_RS15675 0.71 -1.3 3,275,194 - BWI76_RS15675 0.71 -2.6 3,275,194 - BWI76_RS15675 0.71 +1.1 3,275,221 - BWI76_RS15675 0.82 -1.2 3,275,221 - BWI76_RS15675 0.82 +2.1 3,275,278 + +1.2 3,275,322 - +0.3
Or see this region's nucleotide sequence