Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14455 and BWI76_RS14460 are separated by 132 nucleotides BWI76_RS14460 and BWI76_RS14465 are separated by 329 nucleotides BWI76_RS14465 and BWI76_RS14470 are separated by 67 nucleotides
BWI76_RS14455: BWI76_RS14455 - aspartate aminotransferase family protein, at 3,015,936 to 3,017,279
_RS14455
BWI76_RS14460: BWI76_RS14460 - MurR/RpiR family transcriptional regulator, at 3,017,412 to 3,018,269
_RS14460
BWI76_RS14465: BWI76_RS14465 - transcriptional regulator, at 3,018,599 to 3,019,042
_RS14465
BWI76_RS14470: BWI76_RS14470 - L-serine ammonia-lyase, at 3,019,110 to 3,020,477
_RS14470
Position (kb)
3017
3018
3019 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3016.431 kb on + strand, within BWI76_RS14455 at 3016.439 kb on - strand, within BWI76_RS14455 at 3016.509 kb on - strand, within BWI76_RS14455 at 3016.509 kb on - strand, within BWI76_RS14455 at 3016.630 kb on - strand, within BWI76_RS14455 at 3017.085 kb on + strand, within BWI76_RS14455 at 3017.087 kb on - strand, within BWI76_RS14455 at 3017.187 kb on + strand at 3017.245 kb on - strand at 3017.972 kb on - strand, within BWI76_RS14460 at 3018.128 kb on + strand, within BWI76_RS14460 at 3018.128 kb on + strand, within BWI76_RS14460 at 3018.223 kb on + strand at 3018.286 kb on - strand at 3018.324 kb on - strand at 3018.375 kb on - strand at 3018.387 kb on - strand at 3018.395 kb on - strand at 3018.422 kb on - strand at 3018.614 kb on + strand at 3018.617 kb on - strand at 3018.617 kb on - strand at 3018.643 kb on + strand at 3018.671 kb on + strand, within BWI76_RS14465 at 3018.671 kb on + strand, within BWI76_RS14465 at 3018.726 kb on + strand, within BWI76_RS14465 at 3018.759 kb on + strand, within BWI76_RS14465 at 3018.967 kb on + strand, within BWI76_RS14465 at 3018.968 kb on + strand, within BWI76_RS14465 at 3018.970 kb on + strand, within BWI76_RS14465 at 3018.983 kb on + strand, within BWI76_RS14465 at 3018.983 kb on + strand, within BWI76_RS14465 at 3018.983 kb on + strand, within BWI76_RS14465 at 3018.991 kb on - strand, within BWI76_RS14465 at 3019.003 kb on - strand at 3019.004 kb on - strand at 3019.050 kb on + strand at 3019.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 3,016,431 + BWI76_RS14455 0.37 -0.0 3,016,439 - BWI76_RS14455 0.37 +0.6 3,016,509 - BWI76_RS14455 0.43 -0.1 3,016,509 - BWI76_RS14455 0.43 -0.4 3,016,630 - BWI76_RS14455 0.52 +0.4 3,017,085 + BWI76_RS14455 0.85 -1.2 3,017,087 - BWI76_RS14455 0.86 -1.7 3,017,187 + +1.4 3,017,245 - -0.2 3,017,972 - BWI76_RS14460 0.65 -0.8 3,018,128 + BWI76_RS14460 0.83 -3.3 3,018,128 + BWI76_RS14460 0.83 -1.3 3,018,223 + +2.0 3,018,286 - +0.4 3,018,324 - +1.0 3,018,375 - -3.2 3,018,387 - -0.3 3,018,395 - -1.0 3,018,422 - +0.4 3,018,614 + -0.6 3,018,617 - +0.8 3,018,617 - -1.3 3,018,643 + +0.8 3,018,671 + BWI76_RS14465 0.16 +0.6 3,018,671 + BWI76_RS14465 0.16 +3.3 3,018,726 + BWI76_RS14465 0.29 +1.1 3,018,759 + BWI76_RS14465 0.36 -3.4 3,018,967 + BWI76_RS14465 0.83 +0.1 3,018,968 + BWI76_RS14465 0.83 -1.2 3,018,970 + BWI76_RS14465 0.84 +1.2 3,018,983 + BWI76_RS14465 0.86 -2.2 3,018,983 + BWI76_RS14465 0.86 -0.2 3,018,983 + BWI76_RS14465 0.86 -0.3 3,018,991 - BWI76_RS14465 0.88 +0.3 3,019,003 - +0.4 3,019,004 - -1.8 3,019,050 + -0.2 3,019,218 - +1.3
Or see this region's nucleotide sequence