Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13815 and BWI76_RS13820 are separated by 322 nucleotides BWI76_RS13820 and BWI76_RS13825 overlap by 8 nucleotides BWI76_RS13825 and BWI76_RS13830 are separated by 152 nucleotides
BWI76_RS13815: BWI76_RS13815 - ABC transporter, at 2,881,554 to 2,883,266
_RS13815
BWI76_RS13820: BWI76_RS13820 - hypothetical protein, at 2,883,589 to 2,884,269
_RS13820
BWI76_RS13825: BWI76_RS13825 - hypothetical protein, at 2,884,262 to 2,884,957
_RS13825
BWI76_RS13830: BWI76_RS13830 - NAD-dependent malic enzyme, at 2,885,110 to 2,886,807
_RS13830
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2882.654 kb on + strand, within BWI76_RS13815 at 2883.045 kb on - strand, within BWI76_RS13815 at 2883.045 kb on - strand, within BWI76_RS13815 at 2883.080 kb on + strand, within BWI76_RS13815 at 2883.088 kb on - strand, within BWI76_RS13815 at 2883.250 kb on + strand at 2883.405 kb on + strand at 2883.405 kb on + strand at 2883.413 kb on - strand at 2883.413 kb on - strand at 2883.413 kb on - strand at 2883.502 kb on + strand at 2883.513 kb on - strand at 2883.521 kb on - strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.534 kb on + strand at 2883.534 kb on + strand at 2883.534 kb on + strand at 2883.541 kb on - strand at 2883.542 kb on - strand at 2883.548 kb on + strand at 2883.556 kb on - strand at 2883.556 kb on - strand at 2883.561 kb on - strand at 2883.566 kb on + strand at 2883.566 kb on + strand at 2883.569 kb on - strand at 2883.571 kb on + strand at 2883.571 kb on + strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.579 kb on - strand at 2883.579 kb on - strand at 2883.579 kb on - strand at 2883.579 kb on - strand at 2883.584 kb on - strand at 2883.651 kb on + strand at 2883.652 kb on - strand at 2883.652 kb on - strand at 2883.652 kb on - strand at 2883.652 kb on - strand at 2883.676 kb on - strand, within BWI76_RS13820 at 2883.676 kb on - strand, within BWI76_RS13820 at 2883.676 kb on - strand, within BWI76_RS13820 at 2883.676 kb on - strand, within BWI76_RS13820 at 2884.102 kb on - strand, within BWI76_RS13820 at 2884.352 kb on - strand, within BWI76_RS13825 at 2884.985 kb on + strand at 2885.034 kb on + strand at 2885.034 kb on + strand at 2885.034 kb on + strand at 2885.068 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.142 kb on - strand at 2885.155 kb on - strand at 2885.176 kb on - strand at 2885.222 kb on + strand at 2885.222 kb on + strand at 2885.224 kb on + strand at 2885.230 kb on - strand at 2885.230 kb on - strand at 2885.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,882,654 + BWI76_RS13815 0.64 +0.3 2,883,045 - BWI76_RS13815 0.87 +0.0 2,883,045 - BWI76_RS13815 0.87 -2.9 2,883,080 + BWI76_RS13815 0.89 -0.9 2,883,088 - BWI76_RS13815 0.90 -1.2 2,883,250 + -0.9 2,883,405 + -1.1 2,883,405 + -0.2 2,883,413 - -3.2 2,883,413 - +0.9 2,883,413 - -2.2 2,883,502 + +0.5 2,883,513 - +1.0 2,883,521 - +1.4 2,883,533 + -0.6 2,883,533 + -4.1 2,883,533 + -0.9 2,883,533 + -2.4 2,883,533 + -3.9 2,883,533 + -0.2 2,883,533 + -0.9 2,883,533 + +2.8 2,883,534 + -1.5 2,883,534 + -0.5 2,883,534 + +1.2 2,883,541 - +1.0 2,883,542 - -2.4 2,883,548 + +0.1 2,883,556 - -2.4 2,883,556 - -3.0 2,883,561 - +1.1 2,883,566 + -0.9 2,883,566 + +1.5 2,883,569 - +1.5 2,883,571 + +1.7 2,883,571 + +1.6 2,883,574 - -1.0 2,883,574 - +0.3 2,883,574 - +0.1 2,883,574 - -0.8 2,883,574 - +0.8 2,883,574 - +0.7 2,883,574 - -0.0 2,883,574 - -2.0 2,883,579 - -1.0 2,883,579 - -0.6 2,883,579 - -2.6 2,883,579 - -0.4 2,883,584 - -1.2 2,883,651 + -0.2 2,883,652 - +1.8 2,883,652 - -2.4 2,883,652 - +3.1 2,883,652 - -2.4 2,883,676 - BWI76_RS13820 0.13 +2.7 2,883,676 - BWI76_RS13820 0.13 -0.0 2,883,676 - BWI76_RS13820 0.13 -0.6 2,883,676 - BWI76_RS13820 0.13 -1.6 2,884,102 - BWI76_RS13820 0.75 -1.9 2,884,352 - BWI76_RS13825 0.13 +0.4 2,884,985 + -0.4 2,885,034 + +0.3 2,885,034 + -0.6 2,885,034 + +0.2 2,885,068 + -2.2 2,885,070 + -0.3 2,885,070 + +0.9 2,885,070 + -1.7 2,885,070 + -3.5 2,885,070 + +1.4 2,885,070 + +2.1 2,885,070 + +0.1 2,885,070 + +0.7 2,885,070 + -2.1 2,885,070 + -1.0 2,885,070 + -1.0 2,885,070 + +0.9 2,885,070 + -0.4 2,885,070 + -0.8 2,885,070 + +1.9 2,885,070 + +0.8 2,885,070 + +0.4 2,885,070 + -0.6 2,885,070 + +0.8 2,885,070 + +0.5 2,885,070 + -2.6 2,885,070 + -0.2 2,885,070 + +0.2 2,885,070 + -1.0 2,885,070 + -0.2 2,885,070 + +0.0 2,885,070 + +1.4 2,885,070 + +1.7 2,885,070 + -0.0 2,885,070 + -0.6 2,885,070 + +0.6 2,885,070 + +1.0 2,885,070 + +2.5 2,885,070 + +0.7 2,885,070 + -0.4 2,885,078 - +1.5 2,885,078 - -1.7 2,885,078 - -2.3 2,885,078 - +4.0 2,885,142 - +1.0 2,885,155 - +0.5 2,885,176 - +0.5 2,885,222 + -0.1 2,885,222 + -2.0 2,885,224 + -1.0 2,885,230 - -1.9 2,885,230 - +1.3 2,885,230 - +2.0
Or see this region's nucleotide sequence