Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13460 and BWI76_RS13465 are separated by 16 nucleotides BWI76_RS13465 and BWI76_RS13470 are separated by 8 nucleotides BWI76_RS13470 and BWI76_RS13475 overlap by 4 nucleotides BWI76_RS13475 and BWI76_RS13480 are separated by 276 nucleotides
BWI76_RS13460: BWI76_RS13460 - ABC transporter substrate-binding protein, at 2,807,184 to 2,808,782
_RS13460
BWI76_RS13465: BWI76_RS13465 - hypothetical protein, at 2,808,799 to 2,809,032
_RS13465
BWI76_RS13470: BWI76_RS13470 - transporter, at 2,809,041 to 2,809,490
_RS13470
BWI76_RS13475: BWI76_RS13475 - N-acetyltransferase, at 2,809,487 to 2,810,005
_RS13475
BWI76_RS13480: BWI76_RS13480 - XRE family transcriptional regulator, at 2,810,282 to 2,810,857
_RS13480
Position (kb) 2809
2810 Strain fitness (log2 ratio) -1
0
1
2 at 2808.065 kb on + strand, within BWI76_RS13460 at 2808.324 kb on + strand, within BWI76_RS13460 at 2808.676 kb on + strand at 2808.909 kb on - strand, within BWI76_RS13465 at 2809.194 kb on + strand, within BWI76_RS13470 at 2809.585 kb on + strand at 2809.723 kb on - strand, within BWI76_RS13475 at 2810.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,808,065 + BWI76_RS13460 0.55 -0.3 2,808,324 + BWI76_RS13460 0.71 -0.6 2,808,676 + +0.8 2,808,909 - BWI76_RS13465 0.47 -1.0 2,809,194 + BWI76_RS13470 0.34 -1.1 2,809,585 + -0.3 2,809,723 - BWI76_RS13475 0.45 +2.7 2,810,105 + +0.2
Or see this region's nucleotide sequence