Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13470

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13460 and BWI76_RS13465 are separated by 16 nucleotidesBWI76_RS13465 and BWI76_RS13470 are separated by 8 nucleotidesBWI76_RS13470 and BWI76_RS13475 overlap by 4 nucleotidesBWI76_RS13475 and BWI76_RS13480 are separated by 276 nucleotides BWI76_RS13460: BWI76_RS13460 - ABC transporter substrate-binding protein, at 2,807,184 to 2,808,782 _RS13460 BWI76_RS13465: BWI76_RS13465 - hypothetical protein, at 2,808,799 to 2,809,032 _RS13465 BWI76_RS13470: BWI76_RS13470 - transporter, at 2,809,041 to 2,809,490 _RS13470 BWI76_RS13475: BWI76_RS13475 - N-acetyltransferase, at 2,809,487 to 2,810,005 _RS13475 BWI76_RS13480: BWI76_RS13480 - XRE family transcriptional regulator, at 2,810,282 to 2,810,857 _RS13480 Position (kb) 2809 2810Strain fitness (log2 ratio) -1 0 1 2at 2808.065 kb on + strand, within BWI76_RS13460at 2808.324 kb on + strand, within BWI76_RS13460at 2808.676 kb on + strandat 2808.909 kb on - strand, within BWI76_RS13465at 2809.194 kb on + strand, within BWI76_RS13470at 2809.585 kb on + strandat 2809.723 kb on - strand, within BWI76_RS13475at 2810.105 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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2,808,065 + BWI76_RS13460 0.55 -0.3
2,808,324 + BWI76_RS13460 0.71 -0.6
2,808,676 + +0.8
2,808,909 - BWI76_RS13465 0.47 -1.0
2,809,194 + BWI76_RS13470 0.34 -1.1
2,809,585 + -0.3
2,809,723 - BWI76_RS13475 0.45 +2.7
2,810,105 + +0.2

Or see this region's nucleotide sequence