Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13415

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13410 and BWI76_RS13415 are separated by 1 nucleotidesBWI76_RS13415 and BWI76_RS13420 overlap by 11 nucleotidesBWI76_RS13420 and BWI76_RS13425 are separated by 22 nucleotides BWI76_RS13410: BWI76_RS13410 - polyamine ABC transporter ATP-binding protein, at 2,797,268 to 2,798,278 _RS13410 BWI76_RS13415: BWI76_RS13415 - spermidine/putrescine ABC transporter permease, at 2,798,280 to 2,799,209 _RS13415 BWI76_RS13420: BWI76_RS13420 - ABC transporter permease, at 2,799,199 to 2,800,005 _RS13420 BWI76_RS13425: BWI76_RS13425 - 1-pyrroline dehydrogenase, at 2,800,028 to 2,801,455 _RS13425 Position (kb) 2798 2799 2800Strain fitness (log2 ratio) -3 -2 -1 0 1at 2797.769 kb on - strand, within BWI76_RS13410at 2797.780 kb on + strand, within BWI76_RS13410at 2797.839 kb on + strand, within BWI76_RS13410at 2798.327 kb on + strandat 2798.475 kb on - strand, within BWI76_RS13415at 2798.596 kb on + strand, within BWI76_RS13415at 2798.604 kb on - strand, within BWI76_RS13415at 2798.693 kb on - strand, within BWI76_RS13415at 2799.088 kb on + strand, within BWI76_RS13415at 2799.296 kb on - strand, within BWI76_RS13420at 2799.351 kb on + strand, within BWI76_RS13420at 2800.018 kb on + strandat 2800.059 kb on - strandat 2800.147 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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2,797,769 - BWI76_RS13410 0.50 +0.5
2,797,780 + BWI76_RS13410 0.51 -0.3
2,797,839 + BWI76_RS13410 0.56 +0.1
2,798,327 + +0.9
2,798,475 - BWI76_RS13415 0.21 -2.6
2,798,596 + BWI76_RS13415 0.34 +0.2
2,798,604 - BWI76_RS13415 0.35 -3.0
2,798,693 - BWI76_RS13415 0.44 +0.0
2,799,088 + BWI76_RS13415 0.87 -1.3
2,799,296 - BWI76_RS13420 0.12 -0.5
2,799,351 + BWI76_RS13420 0.19 +0.0
2,800,018 + +0.7
2,800,059 - -1.3
2,800,147 + -0.1

Or see this region's nucleotide sequence