Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13220

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13215 and BWI76_RS13220 are separated by 243 nucleotidesBWI76_RS13220 and BWI76_RS13225 are separated by 86 nucleotides BWI76_RS13215: BWI76_RS13215 - type I glyceraldehyde-3-phosphate dehydrogenase, at 2,758,236 to 2,759,234 _RS13215 BWI76_RS13220: BWI76_RS13220 - N-acetyltransferase, at 2,759,478 to 2,759,972 _RS13220 BWI76_RS13225: BWI76_RS13225 - hypothetical protein, at 2,760,059 to 2,760,985 _RS13225 Position (kb) 2759 2760Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2758.950 kb on - strand, within BWI76_RS13215at 2759.150 kb on + strandat 2759.150 kb on + strandat 2759.153 kb on + strandat 2759.158 kb on - strandat 2759.204 kb on + strandat 2759.454 kb on + strandat 2759.524 kb on - strandat 2759.681 kb on - strand, within BWI76_RS13220at 2759.835 kb on + strand, within BWI76_RS13220at 2760.021 kb on - strandat 2760.034 kb on - strandat 2760.103 kb on - strandat 2760.287 kb on + strand, within BWI76_RS13225at 2760.622 kb on + strand, within BWI76_RS13225at 2760.635 kb on - strand, within BWI76_RS13225at 2760.796 kb on + strand, within BWI76_RS13225at 2760.927 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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2,758,950 - BWI76_RS13215 0.71 -0.7
2,759,150 + -1.3
2,759,150 + +0.9
2,759,153 + +0.5
2,759,158 - -2.0
2,759,204 + +0.9
2,759,454 + +1.8
2,759,524 - +2.3
2,759,681 - BWI76_RS13220 0.41 -0.3
2,759,835 + BWI76_RS13220 0.72 -2.8
2,760,021 - -2.7
2,760,034 - -2.7
2,760,103 - -0.5
2,760,287 + BWI76_RS13225 0.25 +0.4
2,760,622 + BWI76_RS13225 0.61 -0.2
2,760,635 - BWI76_RS13225 0.62 +0.3
2,760,796 + BWI76_RS13225 0.80 -0.3
2,760,927 + +0.0

Or see this region's nucleotide sequence