Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13185

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13180 and BWI76_RS13185 are separated by 19 nucleotidesBWI76_RS13185 and BWI76_RS13190 overlap by 1 nucleotidesBWI76_RS13190 and BWI76_RS13195 are separated by 177 nucleotides BWI76_RS13180: BWI76_RS13180 - multidrug transporter EmrA, at 2,750,382 to 2,751,467 _RS13180 BWI76_RS13185: BWI76_RS13185 - MFS transporter, at 2,751,487 to 2,753,139 _RS13185 BWI76_RS13190: BWI76_RS13190 - MarR family transcriptional regulator, at 2,753,139 to 2,753,570 _RS13190 BWI76_RS13195: BWI76_RS13195 - glutamate dehydrogenase, at 2,753,748 to 2,755,022 _RS13195 Position (kb) 2751 2752 2753 2754Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2750.911 kb on + strand, within BWI76_RS13180at 2750.945 kb on - strand, within BWI76_RS13180at 2751.173 kb on + strand, within BWI76_RS13180at 2751.181 kb on - strand, within BWI76_RS13180at 2751.449 kb on - strandat 2751.889 kb on - strand, within BWI76_RS13185at 2752.351 kb on - strand, within BWI76_RS13185at 2752.508 kb on - strand, within BWI76_RS13185at 2753.635 kb on - strandat 2753.659 kb on - strandat 2753.974 kb on + strand, within BWI76_RS13195

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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2,750,911 + BWI76_RS13180 0.49 +1.3
2,750,945 - BWI76_RS13180 0.52 -1.6
2,751,173 + BWI76_RS13180 0.73 -2.1
2,751,181 - BWI76_RS13180 0.74 -0.0
2,751,449 - -2.5
2,751,889 - BWI76_RS13185 0.24 +0.3
2,752,351 - BWI76_RS13185 0.52 -1.5
2,752,508 - BWI76_RS13185 0.62 -4.2
2,753,635 - +0.6
2,753,659 - +1.2
2,753,974 + BWI76_RS13195 0.18 +0.0

Or see this region's nucleotide sequence