Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12890 and BWI76_RS12895 are separated by 168 nucleotides BWI76_RS12895 and BWI76_RS12900 are separated by 194 nucleotides BWI76_RS12900 and BWI76_RS12905 are separated by 265 nucleotides
BWI76_RS12890: BWI76_RS12890 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 2,685,607 to 2,686,077
_RS12890
BWI76_RS12895: BWI76_RS12895 - hypothetical protein, at 2,686,246 to 2,686,543
_RS12895
BWI76_RS12900: BWI76_RS12900 - hypothetical protein, at 2,686,738 to 2,688,354
_RS12900
BWI76_RS12905: BWI76_RS12905 - LysR family transcriptional regulator, at 2,688,620 to 2,689,588
_RS12905
Position (kb)
2686
2687
2688
2689 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2685.903 kb on + strand, within BWI76_RS12890 at 2685.958 kb on - strand, within BWI76_RS12890 at 2686.253 kb on - strand at 2686.259 kb on - strand at 2686.259 kb on - strand at 2686.322 kb on - strand, within BWI76_RS12895 at 2686.322 kb on - strand, within BWI76_RS12895 at 2686.394 kb on + strand, within BWI76_RS12895 at 2686.432 kb on - strand, within BWI76_RS12895 at 2686.434 kb on - strand, within BWI76_RS12895 at 2686.469 kb on - strand, within BWI76_RS12895 at 2686.546 kb on - strand at 2686.620 kb on + strand at 2686.620 kb on + strand at 2686.620 kb on + strand at 2686.620 kb on + strand at 2686.628 kb on - strand at 2686.628 kb on - strand at 2686.727 kb on - strand at 2686.727 kb on - strand at 2686.733 kb on - strand at 2686.906 kb on - strand, within BWI76_RS12900 at 2686.932 kb on + strand, within BWI76_RS12900 at 2687.030 kb on - strand, within BWI76_RS12900 at 2687.030 kb on - strand, within BWI76_RS12900 at 2687.035 kb on - strand, within BWI76_RS12900 at 2687.061 kb on - strand, within BWI76_RS12900 at 2687.521 kb on - strand, within BWI76_RS12900 at 2687.942 kb on + strand, within BWI76_RS12900 at 2687.942 kb on + strand, within BWI76_RS12900 at 2688.189 kb on - strand, within BWI76_RS12900 at 2688.189 kb on - strand, within BWI76_RS12900 at 2688.202 kb on + strand at 2688.210 kb on - strand at 2688.256 kb on + strand at 2688.286 kb on + strand at 2688.286 kb on + strand at 2688.294 kb on - strand at 2688.300 kb on - strand at 2688.304 kb on + strand at 2688.306 kb on - strand at 2688.310 kb on - strand at 2688.310 kb on - strand at 2688.318 kb on + strand at 2688.321 kb on - strand at 2688.326 kb on - strand at 2688.326 kb on - strand at 2688.350 kb on + strand at 2688.350 kb on + strand at 2688.350 kb on + strand at 2688.358 kb on - strand at 2688.358 kb on - strand at 2688.358 kb on - strand at 2688.365 kb on + strand at 2688.375 kb on - strand at 2688.429 kb on + strand at 2688.436 kb on + strand at 2688.437 kb on - strand at 2688.444 kb on + strand at 2688.444 kb on - strand at 2688.444 kb on - strand at 2688.446 kb on - strand at 2688.452 kb on - strand at 2688.452 kb on - strand at 2688.452 kb on - strand at 2688.454 kb on + strand at 2688.567 kb on + strand at 2688.577 kb on + strand at 2688.580 kb on - strand at 2688.601 kb on + strand at 2688.646 kb on - strand at 2688.669 kb on - strand at 2688.690 kb on - strand at 2688.711 kb on - strand at 2688.711 kb on - strand at 2688.711 kb on - strand at 2688.713 kb on + strand at 2688.721 kb on - strand, within BWI76_RS12905 at 2688.721 kb on - strand, within BWI76_RS12905 at 2688.746 kb on - strand, within BWI76_RS12905 at 2688.770 kb on - strand, within BWI76_RS12905 at 2688.781 kb on + strand, within BWI76_RS12905 at 2688.804 kb on - strand, within BWI76_RS12905 at 2688.804 kb on - strand, within BWI76_RS12905 at 2688.804 kb on - strand, within BWI76_RS12905 at 2688.804 kb on - strand, within BWI76_RS12905 at 2688.809 kb on - strand, within BWI76_RS12905 at 2688.811 kb on + strand, within BWI76_RS12905 at 2688.838 kb on - strand, within BWI76_RS12905 at 2688.839 kb on - strand, within BWI76_RS12905 at 2688.855 kb on + strand, within BWI76_RS12905 at 2688.855 kb on + strand, within BWI76_RS12905 at 2688.860 kb on + strand, within BWI76_RS12905 at 2688.863 kb on - strand at 2688.863 kb on - strand, within BWI76_RS12905 at 2688.863 kb on - strand, within BWI76_RS12905 at 2688.863 kb on - strand, within BWI76_RS12905 at 2688.867 kb on - strand, within BWI76_RS12905 at 2688.868 kb on - strand, within BWI76_RS12905 at 2688.868 kb on - strand, within BWI76_RS12905 at 2688.868 kb on - strand, within BWI76_RS12905 at 2688.868 kb on - strand, within BWI76_RS12905 at 2688.903 kb on - strand, within BWI76_RS12905 at 2688.911 kb on - strand, within BWI76_RS12905 at 2688.981 kb on + strand, within BWI76_RS12905 at 2688.989 kb on - strand, within BWI76_RS12905 at 2688.989 kb on - strand, within BWI76_RS12905 at 2689.008 kb on - strand, within BWI76_RS12905 at 2689.014 kb on + strand, within BWI76_RS12905 at 2689.016 kb on + strand, within BWI76_RS12905 at 2689.024 kb on + strand, within BWI76_RS12905 at 2689.028 kb on - strand, within BWI76_RS12905 at 2689.031 kb on + strand, within BWI76_RS12905 at 2689.039 kb on + strand, within BWI76_RS12905 at 2689.039 kb on + strand, within BWI76_RS12905 at 2689.047 kb on - strand, within BWI76_RS12905 at 2689.047 kb on - strand, within BWI76_RS12905 at 2689.047 kb on - strand, within BWI76_RS12905 at 2689.047 kb on - strand, within BWI76_RS12905 at 2689.106 kb on - strand, within BWI76_RS12905 at 2689.106 kb on - strand, within BWI76_RS12905 at 2689.106 kb on - strand, within BWI76_RS12905 at 2689.108 kb on + strand, within BWI76_RS12905 at 2689.108 kb on + strand, within BWI76_RS12905 at 2689.119 kb on + strand, within BWI76_RS12905 at 2689.121 kb on + strand, within BWI76_RS12905 at 2689.126 kb on + strand, within BWI76_RS12905 at 2689.126 kb on + strand, within BWI76_RS12905 at 2689.127 kb on - strand, within BWI76_RS12905 at 2689.131 kb on + strand, within BWI76_RS12905 at 2689.207 kb on + strand, within BWI76_RS12905 at 2689.265 kb on + strand, within BWI76_RS12905
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,685,903 + BWI76_RS12890 0.63 +2.0 2,685,958 - BWI76_RS12890 0.75 +0.9 2,686,253 - -3.5 2,686,259 - -0.8 2,686,259 - +3.6 2,686,322 - BWI76_RS12895 0.26 +2.6 2,686,322 - BWI76_RS12895 0.26 +2.4 2,686,394 + BWI76_RS12895 0.50 -1.3 2,686,432 - BWI76_RS12895 0.62 -1.2 2,686,434 - BWI76_RS12895 0.63 +1.0 2,686,469 - BWI76_RS12895 0.75 -1.7 2,686,546 - +1.6 2,686,620 + +0.7 2,686,620 + -1.8 2,686,620 + -2.5 2,686,620 + +0.3 2,686,628 - -2.3 2,686,628 - +1.0 2,686,727 - -0.3 2,686,727 - +0.5 2,686,733 - -2.6 2,686,906 - BWI76_RS12900 0.10 -0.8 2,686,932 + BWI76_RS12900 0.12 -1.0 2,687,030 - BWI76_RS12900 0.18 +0.5 2,687,030 - BWI76_RS12900 0.18 -0.9 2,687,035 - BWI76_RS12900 0.18 -2.1 2,687,061 - BWI76_RS12900 0.20 +0.6 2,687,521 - BWI76_RS12900 0.48 -0.7 2,687,942 + BWI76_RS12900 0.74 -4.1 2,687,942 + BWI76_RS12900 0.74 +1.0 2,688,189 - BWI76_RS12900 0.90 -3.6 2,688,189 - BWI76_RS12900 0.90 -3.3 2,688,202 + -1.8 2,688,210 - -0.5 2,688,256 + -0.3 2,688,286 + +0.5 2,688,286 + -0.9 2,688,294 - -3.3 2,688,300 - -1.9 2,688,304 + +0.6 2,688,306 - -0.5 2,688,310 - -0.8 2,688,310 - +1.4 2,688,318 + -2.2 2,688,321 - +2.3 2,688,326 - +0.5 2,688,326 - +2.7 2,688,350 + +0.8 2,688,350 + +0.2 2,688,350 + +2.0 2,688,358 - -0.7 2,688,358 - +1.0 2,688,358 - -2.5 2,688,365 + -0.3 2,688,375 - -0.8 2,688,429 + +0.5 2,688,436 + +2.6 2,688,437 - -0.0 2,688,444 + -1.9 2,688,444 - -0.9 2,688,444 - +1.1 2,688,446 - +1.5 2,688,452 - +1.0 2,688,452 - -1.9 2,688,452 - +0.6 2,688,454 + +0.0 2,688,567 + -1.7 2,688,577 + -0.7 2,688,580 - +2.0 2,688,601 + -1.6 2,688,646 - -0.1 2,688,669 - +1.5 2,688,690 - +1.0 2,688,711 - +1.2 2,688,711 - -1.7 2,688,711 - -0.0 2,688,713 + +1.5 2,688,721 - BWI76_RS12905 0.10 +2.2 2,688,721 - BWI76_RS12905 0.10 +0.4 2,688,746 - BWI76_RS12905 0.13 +2.6 2,688,770 - BWI76_RS12905 0.15 +0.6 2,688,781 + BWI76_RS12905 0.17 +1.0 2,688,804 - BWI76_RS12905 0.19 -0.8 2,688,804 - BWI76_RS12905 0.19 -0.4 2,688,804 - BWI76_RS12905 0.19 +1.0 2,688,804 - BWI76_RS12905 0.19 +0.5 2,688,809 - BWI76_RS12905 0.20 -0.6 2,688,811 + BWI76_RS12905 0.20 +3.0 2,688,838 - BWI76_RS12905 0.22 -0.5 2,688,839 - BWI76_RS12905 0.23 -0.3 2,688,855 + BWI76_RS12905 0.24 +4.9 2,688,855 + BWI76_RS12905 0.24 -1.0 2,688,860 + BWI76_RS12905 0.25 +2.3 2,688,863 - +0.5 2,688,863 - BWI76_RS12905 0.25 -1.0 2,688,863 - BWI76_RS12905 0.25 +0.5 2,688,863 - BWI76_RS12905 0.25 +0.4 2,688,867 - BWI76_RS12905 0.25 +0.1 2,688,868 - BWI76_RS12905 0.26 -3.5 2,688,868 - BWI76_RS12905 0.26 -1.1 2,688,868 - BWI76_RS12905 0.26 +1.0 2,688,868 - BWI76_RS12905 0.26 -0.5 2,688,903 - BWI76_RS12905 0.29 -1.0 2,688,911 - BWI76_RS12905 0.30 -1.4 2,688,981 + BWI76_RS12905 0.37 +1.1 2,688,989 - BWI76_RS12905 0.38 -0.2 2,688,989 - BWI76_RS12905 0.38 +0.6 2,689,008 - BWI76_RS12905 0.40 -3.0 2,689,014 + BWI76_RS12905 0.41 +1.7 2,689,016 + BWI76_RS12905 0.41 +0.5 2,689,024 + BWI76_RS12905 0.42 -2.2 2,689,028 - BWI76_RS12905 0.42 -1.7 2,689,031 + BWI76_RS12905 0.42 +0.8 2,689,039 + BWI76_RS12905 0.43 +0.7 2,689,039 + BWI76_RS12905 0.43 -0.1 2,689,047 - BWI76_RS12905 0.44 +0.6 2,689,047 - BWI76_RS12905 0.44 +0.6 2,689,047 - BWI76_RS12905 0.44 +0.1 2,689,047 - BWI76_RS12905 0.44 -0.5 2,689,106 - BWI76_RS12905 0.50 -0.3 2,689,106 - BWI76_RS12905 0.50 -3.3 2,689,106 - BWI76_RS12905 0.50 +0.3 2,689,108 + BWI76_RS12905 0.50 -1.7 2,689,108 + BWI76_RS12905 0.50 -3.0 2,689,119 + BWI76_RS12905 0.51 -1.5 2,689,121 + BWI76_RS12905 0.52 -1.1 2,689,126 + BWI76_RS12905 0.52 +1.4 2,689,126 + BWI76_RS12905 0.52 +1.5 2,689,127 - BWI76_RS12905 0.52 -0.0 2,689,131 + BWI76_RS12905 0.53 -0.6 2,689,207 + BWI76_RS12905 0.61 -3.9 2,689,265 + BWI76_RS12905 0.67 -1.5
Or see this region's nucleotide sequence