Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12110 and BWI76_RS12115 overlap by 4 nucleotides BWI76_RS12115 and BWI76_RS12120 overlap by 8 nucleotides BWI76_RS12120 and BWI76_RS12125 are separated by 0 nucleotides BWI76_RS12125 and BWI76_RS12130 overlap by 1 nucleotides BWI76_RS12130 and BWI76_RS12135 overlap by 4 nucleotides BWI76_RS12135 and BWI76_RS12140 are separated by 248 nucleotides BWI76_RS12140 and BWI76_RS12145 overlap by 1 nucleotides
BWI76_RS12110: BWI76_RS12110 - hypothetical protein, at 2,542,145 to 2,542,930
_RS12110
BWI76_RS12115: BWI76_RS12115 - hypothetical protein, at 2,542,927 to 2,543,130
_RS12115
BWI76_RS12120: BWI76_RS12120 - hypothetical protein, at 2,543,123 to 2,543,308
_RS12120
BWI76_RS12125: BWI76_RS12125 - hypothetical protein, at 2,543,309 to 2,543,521
_RS12125
BWI76_RS12130: BWI76_RS12130 - hypothetical protein, at 2,543,521 to 2,543,778
_RS12130
BWI76_RS12135: BWI76_RS12135 - C-5 cytosine-specific DNA methylase, at 2,543,775 to 2,545,757
_RS12135
BWI76_RS12140: BWI76_RS12140 - hypothetical protein, at 2,546,006 to 2,546,659
_RS12140
BWI76_RS12145: BWI76_RS12145 - hypothetical protein, at 2,546,659 to 2,547,765
_RS12145
Position (kb)
2543
2544
2545
2546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2542.866 kb on + strand at 2543.010 kb on - strand, within BWI76_RS12115 at 2543.010 kb on - strand, within BWI76_RS12115 at 2543.015 kb on - strand, within BWI76_RS12115 at 2543.015 kb on - strand, within BWI76_RS12115 at 2543.107 kb on - strand, within BWI76_RS12115 at 2543.108 kb on - strand, within BWI76_RS12115 at 2543.311 kb on + strand at 2543.311 kb on + strand at 2543.311 kb on + strand at 2543.456 kb on + strand, within BWI76_RS12125 at 2543.490 kb on - strand, within BWI76_RS12125 at 2543.913 kb on - strand at 2543.927 kb on + strand at 2544.089 kb on - strand, within BWI76_RS12135 at 2544.253 kb on + strand at 2544.529 kb on + strand, within BWI76_RS12135 at 2544.604 kb on + strand, within BWI76_RS12135 at 2544.614 kb on - strand, within BWI76_RS12135 at 2544.890 kb on - strand, within BWI76_RS12135 at 2544.890 kb on - strand, within BWI76_RS12135 at 2545.115 kb on + strand, within BWI76_RS12135 at 2545.115 kb on + strand, within BWI76_RS12135 at 2545.327 kb on + strand, within BWI76_RS12135 at 2545.454 kb on - strand, within BWI76_RS12135 at 2545.507 kb on - strand, within BWI76_RS12135 at 2545.579 kb on + strand at 2545.580 kb on + strand at 2545.580 kb on + strand at 2545.588 kb on - strand at 2545.590 kb on + strand at 2545.590 kb on + strand at 2545.598 kb on - strand at 2545.603 kb on - strand at 2545.603 kb on - strand at 2545.603 kb on - strand at 2545.625 kb on + strand at 2545.625 kb on + strand at 2545.635 kb on + strand at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.680 kb on - strand at 2545.681 kb on - strand at 2545.681 kb on - strand at 2545.704 kb on + strand at 2545.709 kb on + strand at 2545.709 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.717 kb on - strand at 2545.722 kb on - strand at 2545.728 kb on + strand at 2545.733 kb on + strand at 2545.738 kb on + strand at 2545.738 kb on + strand at 2545.741 kb on - strand at 2545.741 kb on - strand at 2545.746 kb on - strand at 2545.786 kb on + strand at 2545.811 kb on + strand at 2545.814 kb on - strand at 2545.814 kb on - strand at 2545.819 kb on - strand at 2545.824 kb on + strand at 2545.832 kb on - strand at 2545.840 kb on - strand at 2545.886 kb on + strand at 2545.886 kb on + strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.925 kb on - strand at 2545.930 kb on - strand at 2545.930 kb on - strand at 2545.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,542,866 + -2.7 2,543,010 - BWI76_RS12115 0.41 +0.2 2,543,010 - BWI76_RS12115 0.41 +1.3 2,543,015 - BWI76_RS12115 0.43 +1.6 2,543,015 - BWI76_RS12115 0.43 -0.7 2,543,107 - BWI76_RS12115 0.88 +0.3 2,543,108 - BWI76_RS12115 0.89 -0.0 2,543,311 + -1.2 2,543,311 + -0.4 2,543,311 + -0.8 2,543,456 + BWI76_RS12125 0.69 -1.4 2,543,490 - BWI76_RS12125 0.85 -1.2 2,543,913 - -0.0 2,543,927 + -0.1 2,544,089 - BWI76_RS12135 0.16 -0.6 2,544,253 + -0.0 2,544,529 + BWI76_RS12135 0.38 -3.7 2,544,604 + BWI76_RS12135 0.42 +1.1 2,544,614 - BWI76_RS12135 0.42 -1.0 2,544,890 - BWI76_RS12135 0.56 -0.2 2,544,890 - BWI76_RS12135 0.56 -1.4 2,545,115 + BWI76_RS12135 0.68 +0.6 2,545,115 + BWI76_RS12135 0.68 -2.1 2,545,327 + BWI76_RS12135 0.78 +0.9 2,545,454 - BWI76_RS12135 0.85 -0.4 2,545,507 - BWI76_RS12135 0.87 -1.4 2,545,579 + -2.7 2,545,580 + +0.4 2,545,580 + +0.2 2,545,588 - +1.3 2,545,590 + -1.4 2,545,590 + +1.2 2,545,598 - -3.2 2,545,603 - +1.0 2,545,603 - -0.1 2,545,603 - -0.2 2,545,625 + +0.8 2,545,625 + +0.2 2,545,635 + -0.0 2,545,672 + +0.7 2,545,672 + -0.5 2,545,672 + +1.0 2,545,672 + -3.6 2,545,680 - +1.3 2,545,681 - -1.5 2,545,681 - -0.5 2,545,704 + -0.5 2,545,709 + -0.5 2,545,709 + +1.8 2,545,714 + -0.7 2,545,714 + -1.9 2,545,714 + +0.2 2,545,714 + -0.3 2,545,714 + -0.7 2,545,717 - -0.0 2,545,722 - -1.1 2,545,728 + -0.4 2,545,733 + -3.3 2,545,738 + +0.3 2,545,738 + -0.7 2,545,741 - -1.3 2,545,741 - -1.0 2,545,746 - +1.3 2,545,786 + +0.8 2,545,811 + -1.2 2,545,814 - +1.0 2,545,814 - -1.0 2,545,819 - -0.7 2,545,824 + +1.7 2,545,832 - +2.1 2,545,840 - -0.1 2,545,886 + +0.4 2,545,886 + -1.4 2,545,894 - -0.4 2,545,894 - +0.0 2,545,894 - -0.4 2,545,894 - -1.9 2,545,894 - +2.2 2,545,894 - -0.6 2,545,894 - -0.5 2,545,925 - -0.8 2,545,930 - -2.8 2,545,930 - -1.0 2,545,930 - +0.0
Or see this region's nucleotide sequence