Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12105 and BWI76_RS12110 overlap by 8 nucleotides BWI76_RS12110 and BWI76_RS12115 overlap by 4 nucleotides BWI76_RS12115 and BWI76_RS12120 overlap by 8 nucleotides BWI76_RS12120 and BWI76_RS12125 are separated by 0 nucleotides BWI76_RS12125 and BWI76_RS12130 overlap by 1 nucleotides BWI76_RS12130 and BWI76_RS12135 overlap by 4 nucleotides
BWI76_RS12105: BWI76_RS12105 - hypothetical protein, at 2,541,817 to 2,542,152
_RS12105
BWI76_RS12110: BWI76_RS12110 - hypothetical protein, at 2,542,145 to 2,542,930
_RS12110
BWI76_RS12115: BWI76_RS12115 - hypothetical protein, at 2,542,927 to 2,543,130
_RS12115
BWI76_RS12120: BWI76_RS12120 - hypothetical protein, at 2,543,123 to 2,543,308
_RS12120
BWI76_RS12125: BWI76_RS12125 - hypothetical protein, at 2,543,309 to 2,543,521
_RS12125
BWI76_RS12130: BWI76_RS12130 - hypothetical protein, at 2,543,521 to 2,543,778
_RS12130
BWI76_RS12135: BWI76_RS12135 - C-5 cytosine-specific DNA methylase, at 2,543,775 to 2,545,757
_RS12135
Position (kb)
2542
2543
2544 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2542.011 kb on - strand, within BWI76_RS12105 at 2542.050 kb on + strand, within BWI76_RS12105 at 2542.289 kb on + strand, within BWI76_RS12110 at 2542.297 kb on - strand, within BWI76_RS12110 at 2542.297 kb on - strand at 2542.297 kb on - strand, within BWI76_RS12110 at 2542.297 kb on - strand, within BWI76_RS12110 at 2542.309 kb on + strand, within BWI76_RS12110 at 2542.383 kb on + strand, within BWI76_RS12110 at 2542.613 kb on + strand, within BWI76_RS12110 at 2542.655 kb on - strand, within BWI76_RS12110 at 2542.697 kb on + strand, within BWI76_RS12110 at 2542.866 kb on + strand at 2543.010 kb on - strand, within BWI76_RS12115 at 2543.010 kb on - strand, within BWI76_RS12115 at 2543.015 kb on - strand, within BWI76_RS12115 at 2543.015 kb on - strand, within BWI76_RS12115 at 2543.107 kb on - strand, within BWI76_RS12115 at 2543.108 kb on - strand, within BWI76_RS12115 at 2543.311 kb on + strand at 2543.311 kb on + strand at 2543.311 kb on + strand at 2543.456 kb on + strand, within BWI76_RS12125 at 2543.490 kb on - strand, within BWI76_RS12125 at 2543.913 kb on - strand at 2543.927 kb on + strand at 2544.089 kb on - strand, within BWI76_RS12135
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,542,011 - BWI76_RS12105 0.58 -0.5 2,542,050 + BWI76_RS12105 0.69 -0.4 2,542,289 + BWI76_RS12110 0.18 -3.4 2,542,297 - BWI76_RS12110 0.19 -0.6 2,542,297 - -0.8 2,542,297 - BWI76_RS12110 0.19 -1.2 2,542,297 - BWI76_RS12110 0.19 -0.9 2,542,309 + BWI76_RS12110 0.21 -0.0 2,542,383 + BWI76_RS12110 0.30 -0.7 2,542,613 + BWI76_RS12110 0.60 -0.9 2,542,655 - BWI76_RS12110 0.65 -0.4 2,542,697 + BWI76_RS12110 0.70 +1.6 2,542,866 + -2.7 2,543,010 - BWI76_RS12115 0.41 +0.2 2,543,010 - BWI76_RS12115 0.41 +1.3 2,543,015 - BWI76_RS12115 0.43 +1.6 2,543,015 - BWI76_RS12115 0.43 -0.7 2,543,107 - BWI76_RS12115 0.88 +0.3 2,543,108 - BWI76_RS12115 0.89 -0.0 2,543,311 + -1.2 2,543,311 + -0.4 2,543,311 + -0.8 2,543,456 + BWI76_RS12125 0.69 -1.4 2,543,490 - BWI76_RS12125 0.85 -1.2 2,543,913 - -0.0 2,543,927 + -0.1 2,544,089 - BWI76_RS12135 0.16 -0.6
Or see this region's nucleotide sequence