Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12025 and BWI76_RS12030 overlap by 104 nucleotides BWI76_RS12030 and BWI76_RS12035 are separated by 9 nucleotides BWI76_RS12035 and BWI76_RS12040 are separated by 7 nucleotides BWI76_RS12040 and BWI76_RS12045 overlap by 4 nucleotides BWI76_RS12045 and BWI76_RS12050 overlap by 8 nucleotides
BWI76_RS12025: BWI76_RS12025 - integrase, at 2,529,969 to 2,531,198
_RS12025
BWI76_RS12030: BWI76_RS12030 - hypothetical protein, at 2,531,095 to 2,531,409
_RS12030
BWI76_RS12035: BWI76_RS12035 - hypothetical protein, at 2,531,419 to 2,531,658
_RS12035
BWI76_RS12040: BWI76_RS12040 - hypothetical protein, at 2,531,666 to 2,531,974
_RS12040
BWI76_RS12045: BWI76_RS12045 - hypothetical protein, at 2,531,971 to 2,532,690
_RS12045
BWI76_RS12050: BWI76_RS12050 - hypothetical protein, at 2,532,683 to 2,533,036
_RS12050
Position (kb)
2531
2532 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2530.675 kb on + strand, within BWI76_RS12025 at 2530.727 kb on + strand, within BWI76_RS12025 at 2530.735 kb on - strand, within BWI76_RS12025 at 2531.006 kb on - strand, within BWI76_RS12025 at 2531.085 kb on - strand at 2531.104 kb on + strand at 2531.143 kb on + strand at 2531.149 kb on + strand at 2531.151 kb on - strand at 2531.152 kb on - strand at 2531.226 kb on + strand, within BWI76_RS12030 at 2531.234 kb on - strand at 2531.313 kb on - strand, within BWI76_RS12030 at 2531.423 kb on - strand at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.708 kb on + strand, within BWI76_RS12040 at 2531.983 kb on + strand at 2532.114 kb on - strand, within BWI76_RS12045 at 2532.233 kb on + strand, within BWI76_RS12045 at 2532.627 kb on + strand at 2532.627 kb on + strand at 2532.642 kb on + strand at 2532.645 kb on - strand at 2532.869 kb on + strand, within BWI76_RS12050 at 2532.877 kb on - strand, within BWI76_RS12050 at 2532.937 kb on + strand, within BWI76_RS12050 at 2532.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,530,675 + BWI76_RS12025 0.57 +0.1 2,530,727 + BWI76_RS12025 0.62 -1.2 2,530,735 - BWI76_RS12025 0.62 +0.5 2,531,006 - BWI76_RS12025 0.84 +0.8 2,531,085 - +0.7 2,531,104 + +1.1 2,531,143 + +0.4 2,531,149 + -0.6 2,531,151 - -0.8 2,531,152 - +0.1 2,531,226 + BWI76_RS12030 0.42 +0.4 2,531,234 - +0.4 2,531,313 - BWI76_RS12030 0.69 -1.7 2,531,423 - +1.0 2,531,461 + BWI76_RS12035 0.17 +0.2 2,531,461 + BWI76_RS12035 0.17 -0.3 2,531,461 + BWI76_RS12035 0.17 +0.6 2,531,469 - BWI76_RS12035 0.21 -3.6 2,531,469 - BWI76_RS12035 0.21 +0.7 2,531,469 - BWI76_RS12035 0.21 -0.2 2,531,708 + BWI76_RS12040 0.14 -1.7 2,531,983 + -0.1 2,532,114 - BWI76_RS12045 0.20 +1.0 2,532,233 + BWI76_RS12045 0.36 +0.8 2,532,627 + -4.2 2,532,627 + -3.4 2,532,642 + +3.1 2,532,645 - +0.1 2,532,869 + BWI76_RS12050 0.53 -3.4 2,532,877 - BWI76_RS12050 0.55 +1.2 2,532,937 + BWI76_RS12050 0.72 -0.1 2,532,956 - +2.0
Or see this region's nucleotide sequence