Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11240 and BWI76_RS11245 overlap by 4 nucleotides BWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotides BWI76_RS11250 and BWI76_RS11255 overlap by 17 nucleotides
BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253
_RS11240
BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035
_RS11245
BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889
_RS11250
BWI76_RS11255: BWI76_RS11255 - putrescine/spermidine ABC transporter ATP-binding protein, at 2,373,873 to 2,375,009
_RS11255
Position (kb)
2372
2373
2374 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2371.571 kb on + strand, within BWI76_RS11240 at 2371.715 kb on + strand, within BWI76_RS11240 at 2371.725 kb on + strand, within BWI76_RS11240 at 2371.762 kb on - strand, within BWI76_RS11240 at 2371.867 kb on - strand, within BWI76_RS11240 at 2372.461 kb on - strand, within BWI76_RS11245 at 2372.599 kb on - strand, within BWI76_RS11245 at 2372.922 kb on + strand, within BWI76_RS11245 at 2373.411 kb on + strand, within BWI76_RS11250 at 2373.732 kb on - strand, within BWI76_RS11250 at 2373.837 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,371,571 + BWI76_RS11240 0.35 +1.3 2,371,715 + BWI76_RS11240 0.49 -0.2 2,371,725 + BWI76_RS11240 0.49 -0.1 2,371,762 - BWI76_RS11240 0.53 -0.1 2,371,867 - BWI76_RS11240 0.63 -0.7 2,372,461 - BWI76_RS11245 0.27 -2.2 2,372,599 - BWI76_RS11245 0.44 +0.0 2,372,922 + BWI76_RS11245 0.85 -1.5 2,373,411 + BWI76_RS11250 0.44 +1.8 2,373,732 - BWI76_RS11250 0.82 +0.3 2,373,837 - +0.1
Or see this region's nucleotide sequence